Highly efficient microfluidic device for cell trapping and pairing towards cell-cell communication analysis

Zhu, Jinchi; Wang, Yachao; Peng, Chen; Su, Huiying; Du, Wei; Liu, Bi‐Feng

doi:10.1016/j.snb.2018.12.078

Cited by 23 publications

(20 citation statements)

References 41 publications

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“…Vertical and horizontal traps are two straightforward microstructures for single cell trapping and downstream analysis. Both of them can be fabricated as a high-throughput array for multiple cell isolation when each cell is separated and limited in its own space for further optical detection and analysis (Zhang et al 2014 ; Zhu et al 2019 ; Zhou et al 2020 ), as shown in Fig. 1 B-1.…”

Section: Single-cell Nucleic Acid Analysis Based On Microfluidicsmentioning

confidence: 99%

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Liu

Dong

et al. 2021

Microfluid Nanofluid

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Single-cell nucleic acid analysis aims at discovering the genetic differences between individual cells which is well known as the cellular heterogeneity. This technology facilitates cancer diagnosis, stem cell research, immune system analysis, and other life science applications. The conventional platforms for single-cell nucleic acid analysis more rely on manual operation or bulky devices. Recently, the emerging microfluidic technology has provided a perfect platform for single-cell nucleic acid analysis with the characteristic of accurate and automatic single-cell manipulation. In this review, we briefly summarized the procedure of single-cell nucleic acid analysis including single-cell isolation, single-cell lysis, nucleic acid amplification, and genetic analysis. And then, three representative microfluidic platforms for single-cell nucleic acid analysis are concluded as valve-, microwell-, and droplet-based platforms. Furthermore, we described the state-of-the-art integrated single-cell nucleic acid analysis systems based on the three platforms. Finally, the future development and challenges of microfluidics-based single-cell nucleic acid analysis are discussed as well.

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Section: Single-cell Nucleic Acid Analysis Based On Microfluidicsmentioning

confidence: 99%

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Liu

Dong

et al. 2021

Microfluid Nanofluid

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“…Subsequently, residual cells were washed away, and a different cell suspension was added sequentially in order to pair the predetermined cells. While the chance of cross-contamination was limited, the device offered a slightly low throughput [45]. Abe et al demonstrated a cell pairing system suitable for mounting and positioning and manipulating cells by mechanically sliding a parylene rail films (PRF).…”

Section: Multilayer Soft Lithography Using Pdmsmentioning

confidence: 99%

Latest Updates on the Advancement of Polymer-Based Biomicroelectromechanical Systems for Animal Cell Studies

Garcia-Ramirez

Cerda-Kipper

Alvarez

et al. 2021

Advances in Polymer Technology

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Biological sciences have reached the fundamental unit of life: the cell. Ever-growing field of Biological Microelectromechanical Systems (BioMEMSs) is providing new frontiers in both fundamental cell research and various practical applications in cell-related studies. Among various functions of BioMEMS devices, some of the most fundamental processes that can be carried out in such platforms include cell sorting, cell separation, cell isolation or trapping, cell pairing, cell-cell communication, cell differentiation, cell identification, and cell culture. In this article, we review each mentioned application in great details highlighting the latest advancements in fabrication strategy, mechanism of operation, and application of these tools. Moreover, the review article covers the shortcomings of each specific application which can open windows of opportunity for improvement of these devices.

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“…The trapping efficiency was up to 70%. Zhu et al [55] also developed a microfluidic device for cell trapping based on hydrodynamic method. For the first step, they tested the capabilities of cell trapping in three types of microstructures, as shown in Figure 5a.…”

Section: Hydrodynamic Trappingmentioning

confidence: 99%

“…It was found that the microstructure of type C was the most efficient in cell trapping, as shown in Figure 5a(ii). The blue bar and red bar represent the trapping efficiency Zhu et al [55] also developed a microfluidic device for cell trapping based on hydrodynamic method. For the first step, they tested the capabilities of cell trapping in three types of microstructures, as shown in Figure 5a.…”

Section: Hydrodynamic Trappingmentioning

confidence: 99%

Recent Development of Microfluidic Technology for Cell Trapping in Single Cell Analysis: A Review

Deng

Guo

2020

Processes

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Microfluidic technology has emerged from the MEMS (Micro-Electro-Mechanical System)-technology as an important research field. During the last decade, various microfluidic technologies have been developed to open up a new era for biological studies. To understand the function of single cells, it is very important to monitor the dynamic behavior of a single cell in a living environment. Cell trapping in single cell analysis is urgently demanded There have been some review papers focusing on drug screen and cell analysis. However, cell trapping in single cell analysis has rarely been covered in the previous reviews. The present paper focuses on recent developments of cell trapping and highlights the mechanisms, governing equations and key parameters affecting the cell trapping efficiency by contact-based and contactless approach. The applications of the cell trapping method are discussed according to their basic research areas, such as biology and tissue engineering. Finally, the paper highlights the most promising cell trapping method for this research area.

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Highly efficient microfluidic device for cell trapping and pairing towards cell-cell communication analysis

Cited by 23 publications

References 41 publications

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

Latest Updates on the Advancement of Polymer-Based Biomicroelectromechanical Systems for Animal Cell Studies

Recent Development of Microfluidic Technology for Cell Trapping in Single Cell Analysis: A Review

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