Highly efficient production of chitooligosaccharides by enzymes mined directly from the marine metagenome

Guan, Feifei; Han, Yanshuo; Yan, Kai; Zhang, Yan; Zhang, Zhifang; Wu, Ningfeng; Tian, Jian

doi:10.1016/j.carbpol.2020.115909

Cited by 24 publications

(15 citation statements)

References 44 publications

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“…QD1 from the coast of Qingdao, China, displayed a broad pH stability of 5.0-10.0 [12]. An endo-type chitosanase CHIS5 mined from the metagenome of marine microorganisms has been used to degrade acetylated chitosan for efficient production of COS associating with the chitin deacetylase CDA20 [13].…”

Section: Introductionmentioning

confidence: 99%

Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine Renibacterium sp. Suitable for Chitooligosaccharides Preparation

et al. 2021

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(1) Background: Chitooligosaccharides (COS) have numerous applications due to their excellent properties. Chitosan hydrolysis using chitosanases has been proposed as an advisable method for COS preparation. Although many chitosanases from various sources have been identified, the cold-adapted ones with high stability are still rather rare but required. (2) Methods: A novel chitosanase named CsnY from marine bacterium Renibacterium sp. Y82 was expressed in Escherichia coli, following sequence analysis. Then, the characterizations of recombinant CsnY purified through Ni–NTA affinity chromatography were conducted, including effects of pH and temperature, effects of metal ions and chemicals, and final product analysis. (3) Results: The GH46 family chitosanase CsnY possessed promising thermostability at broad temperature range (0–50 °C), and with optimal activity at 40 °C and pH 6.0, especially showing relatively high activity (over 80% of its maximum activity) at low temperatures (20–30 °C), which demonstrated the cold-adapted property. Common metal ions or chemicals had no obvious effect on CsnY except Mn2+ and Co2+. Finally, CsnY was determined to be an endo-type chitosanase generating chitodisaccharides and -trisaccharides as main products, whose total concentration reached 56.74 mM within 2 h against 2% (w/v) initial chitosan substrate. (4) Conclusions: The results suggest the cold-adapted CsnY with favorable stability has desirable potential for the industrial production of COS.

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Section: Introductionmentioning

confidence: 99%

Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine Renibacterium sp. Suitable for Chitooligosaccharides Preparation

et al. 2021

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“…In particular, the chitosanase OUC‐CsnPT degraded chitosan into monomers and dimers, and when combined with rare deacetylase that concurrently removed the acetyl groups from GlcNAc and chitobiose (Guan et al, 2020), it was possible to hydrolyze part of acetyl chitosan to COSs. The mixed state of COSs and GlcN was caused by the existence of acetyl groups in chitosan.…”

Section: Discussionmentioning

confidence: 99%

“…Chitosanases in the GH46 family, which have excellent catalytic potential, have been studied extensively among these families (Heggset et al, 2010). Over the past decades, a large number of chitosanases have been identified, cloned, characterized, and attempted to improve the hydrolysis efficiency of chitosan (Han et al, 2020; H. Sun et al, 2018; Viens et al, 2015). For example, a GH46 chitosanase (BaCsn46B) from Bacillus amyloliquefaciens ECU08 with extremely excellent enzymatic activity (2380.5 U/mg) was identified and biochemically characterized (Luo et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Heterogenously‐expressed chitosanase combining a green ball milling method for enzymatic degradation

Sun

Guo

et al. 2022

Food Bioengineering

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Chitooligosaccharides (COSs) and D-glucosamine (GlcN), the most valuable biomolecules due to their various physiological functions, can be available inexpensively from chitosan by bio-enzymatic degradation, in which chitosanases play a key role. On the other hand, pretreatment of chitosan is the necessary procedure in the bioproduction by enzymatic hydrolysis. Therefore, the green and efficient pretreatment strategy as well as superior enzyme activity are the most critical aspects in scaled up production. This study investigated environmentally friendly and efficient enzymatic hydrolysis of powdery chitosan (PC) pretreated by ball-milling. First, heterologous expression yielded OUC-CsnPT, a glycoside hydrolase family 46 chitosanase with a predominant specific activity of 5346.56 U/mg at pH 6.0°C and 45°C. The endo-type chitosanase OUC-CsnPT hydrolyzed chitosan to produce GlcN and chitobiose [(GlcN) 2 ]. Then, ball-milling was employed to pretreat PC to promote chitosanase OUC-CsnPT hydrolysis, and the effectiveness was characterized by scanning electron microscope, X-ray diffraction, and FTIR-attenuated total reflection. Through the development of the novel route, the yield of COSs and GlcN for ball-milling powdery chitosan increased by 10.8-folds with a concentration of 14.5 μmol/ml. Hence, this technique presents a promising strategy suited to be a straightforward and environmentally friendly option for oligosaccharides production, broadening the theoretical basis for PC biodegradation.

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“…The latter includes heating, sonication, grinding and derivatization (glycolated, reprecipitated and depolymerized) of chitin [100]. Various chitin deacetylases are being investigated from fungi, bacteria, insects, plants and metagenome for use in chitosan production on a commercial scale [101][102][103][104][105][106][107].…”

Section: Biological Methods Of Chitosan Preparationmentioning

confidence: 99%

A review on valorization of chitinous waste

Sharma

Kaur²,

Kaur

et al. 2021

J Polym Res

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The accumulation of waste from sea food industries poses hazards to aquatic life and environment. The shells of crustaceans, which constitute a major chunk of the sea food waste, are the source of chitin, a valuable polysaccharide. The physiochemical properties of chitin make it suitable for several applications, viz. in medical science, waste water treatment, food preservation etc. To explore its potential in uncharted domains, it is desired that this polymer is available in abundance. Chitin can be extracted from the marine sea food waste by chemical or biological methods. Owing to the higher efficiency and short processing time of the chemical method in the recovery of chitin from the marine biomass waste, this method has been adopted in industries in spite of its adverse impact on the environment. On the contrary, the eco-friendly biological method is still restricted to the research laboratories. In view of this, we aim to provide an overview on the sources and physicochemical properties of chitin/chitosan and discuss the methods of their extraction and applications. As the impetus to green technologies in chitin extraction would be beneficial to the environment, this review highlights the advantages of biological methods of chitin extraction over the chemical methods. Though the biological method of chitin extraction offers a wider choice of enzymes or microorganisms in the fermentation processes, it is not without challenges. There is a need to increase the efficiency of the biological process of chitin extraction to make it a key process to harvest the high value biopolymer from marine waste without any damage to the environment and to explore its utility for the welfare of mankind.

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Highly efficient production of chitooligosaccharides by enzymes mined directly from the marine metagenome

Cited by 24 publications

References 44 publications

Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine Renibacterium sp. Suitable for Chitooligosaccharides Preparation

Expression and Characterization of a Novel Cold-Adapted Chitosanase from Marine Renibacterium sp. Suitable for Chitooligosaccharides Preparation

Heterogenously‐expressed chitosanase combining a green ball milling method for enzymatic degradation

A review on valorization of chitinous waste

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