Highly Efficient Synthesis of Type B Gelatin and Low Molecular Weight Chitosan Nanoparticles: Potential Applications as Bioactive Molecule Carriers and Cell-Penetrating Agents

Gonzalez-Melo, Cristina; Garcia-Brand, Andres J.; Quezada, Valentina; Reyes, Luis H.; Muñoz-Camargo, Carolina; Cruz, Juan C.

doi:10.3390/polym13234078

Cited by 10 publications

(8 citation statements)

References 76 publications

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“…Due to the insolubilisation of chitosan, precipitation occurs. To harden the nanoparticles with an average range of 373 ± 71 nm, glutaraldehyde is applied last [ 52 , 93 , 94 ]. This technique has several advantages over other methods, the most notable of which is its ability to create nanoparticles in a single step, in addition to its low costs, low use of electricity, and frequency [ 95 , 96 ].…”

Section: Production Of Chitosan Nanoparticlesmentioning

confidence: 99%

Chitosan Nanoparticles: A Versatile Platform for Biomedical Applications

et al. 2022

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Chitosan is a biodegradable and biocompatible natural polymer that has been extensively explored in recent decades. The Food and Drug Administration has approved chitosan for wound treatment and nutritional use. Furthermore, chitosan has paved the way for advancements in different biomedical applications including as a nanocarrier and tissue-engineering scaffold. Its antibacterial, antioxidant, and haemostatic properties make it an excellent option for wound dressings. Because of its hydrophilic nature, chitosan is an ideal starting material for biocompatible and biodegradable hydrogels. To suit specific application demands, chitosan can be combined with fillers, such as hydroxyapatite, to modify the mechanical characteristics of pH-sensitive hydrogels. Furthermore, the cationic characteristics of chitosan have made it a popular choice for gene delivery and cancer therapy. Thus, the use of chitosan nanoparticles in developing novel drug delivery systems has received special attention. This review aims to provide an overview of chitosan-based nanoparticles, focusing on their versatile properties and different applications in biomedical sciences and engineering.

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Section: Production Of Chitosan Nanoparticlesmentioning

confidence: 99%

Chitosan Nanoparticles: A Versatile Platform for Biomedical Applications

et al. 2022

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“…One of the most employed natural polymers for therapeutic hydrogels is chitosan, a biomaterial that can be obtained from the partial deacetylation of naturally abundant chitin [ 34 , 35 , 36 , 37 ]. Along with a strong inherent adhesiveness and biocompatibility, chitosan exhibits desirable hemostatic, antimicrobial, and analgesic properties, making it an attractive material for numerous applications in nanomedicine [ 38 , 39 ] and regenerative medicine [ 40 ]. Moreover, chitosan can be further engineered for specific functionalities by conjugating functional molecules to the free acetamide and hydroxyl groups linked to the glucopyranose rings in their structure, which are susceptible to nucleophilic attacks [ 37 ].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Characterization of an Injectable and Photo-Responsive Chitosan Methacrylate/Graphene Oxide Hydrogel: Potential Applications in Bone Tissue Adhesion and Repair

et al. 2021

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As life expectancy continues to increase, the inevitable weakening and rupture of bone tissue have grown as concerns in the medical community, thus leading to the need for adhesive materials suitable for bone repair applications. However, current commercially available adhesives face certain drawbacks that prevent proper tissue repair, such as low biocompatibility, poor adhesion to wet surfaces, and the need for high polymerization temperatures. This work aims to develop an injectable and photo-responsive chitosan methacrylate/graphene oxide (ChiMA/GO) adhesive nanocomposite hydrogel of high biocompatibility that is easy to apply by simple extrusion and that offers the possibility for in situ polymer and physiological temperatures. The nanocomposite was thoroughly characterized spectroscopically, microscopically, rheologically, thermally, and through mechanical, textural, and biological assays to fully evaluate its correct synthesis and functionalization and its performance under physiological conditions that mimic those observed in vivo. In addition, a finite element analysis (FEA) simulation was used to evaluate its performance in femur fractures. Results suggest the material’s potential as a bioadhesive, as it can polymerize at room temperature, shows superior stability in physiological media, and is capable of withstanding loads from body weight and movement. Moreover, the material showed remarkable biocompatibility as evidenced by low hemolytic and intermediate platelet aggregation tendencies, and high cytocompatibility when in contact with osteoblasts. The comprehensive studies presented here strongly suggest that the developed hydrogels are promising alternatives to conventional bone adhesives that might be further tested in vivo in the near future.

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“…CNPs were synthesized following the ionic gelation method [ 63 ]. Briefly, 2.4 mg/mL of LMW Chitosan (50–190 kDa, deacetylation degree of 75–85%, CAS 9012-76-4) was dissolved in acetic acid 2% v / v under magnetic stirring for 3 h. This procedure protonates the amine groups of monomers and therefore increases its solubility.…”

Section: Materials and Methodsmentioning

confidence: 99%

Rational Discovery of Antimicrobial Peptides by Means of Artificial Intelligence

et al. 2022

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Antibiotic resistance is a worldwide public health problem due to the costs and mortality rates it generates. However, the large pharmaceutical industries have stopped searching for new antibiotics because of their low profitability, given the rapid replacement rates imposed by the increasingly observed resistance acquired by microorganisms. Alternatively, antimicrobial peptides (AMPs) have emerged as potent molecules with a much lower rate of resistance generation. The discovery of these peptides is carried out through extensive in vitro screenings of either rational or non-rational libraries. These processes are tedious and expensive and generate only a few AMP candidates, most of which fail to show the required activity and physicochemical properties for practical applications. This work proposes implementing an artificial intelligence algorithm to reduce the required experimentation and increase the efficiency of high-activity AMP discovery. Our deep learning (DL) model, called AMPs-Net, outperforms the state-of-the-art method by 8.8% in average precision. Furthermore, it is highly accurate to predict the antibacterial and antiviral capacity of a large number of AMPs. Our search led to identifying two unreported antimicrobial motifs and two novel antimicrobial peptides related to them. Moreover, by coupling DL with molecular dynamics (MD) simulations, we were able to find a multifunctional peptide with promising therapeutic effects. Our work validates our previously proposed pipeline for a more efficient rational discovery of novel AMPs.

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Highly Efficient Synthesis of Type B Gelatin and Low Molecular Weight Chitosan Nanoparticles: Potential Applications as Bioactive Molecule Carriers and Cell-Penetrating Agents

Cited by 10 publications

References 76 publications

Chitosan Nanoparticles: A Versatile Platform for Biomedical Applications

Chitosan Nanoparticles: A Versatile Platform for Biomedical Applications

Preparation and Characterization of an Injectable and Photo-Responsive Chitosan Methacrylate/Graphene Oxide Hydrogel: Potential Applications in Bone Tissue Adhesion and Repair

Rational Discovery of Antimicrobial Peptides by Means of Artificial Intelligence

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