Highly efficient system for plant regeneration from leaf and stem explants in Dahlia

Abstract

Protocol of plant regeneration from leaf and stem explants was established in dahlia (Dahlia×pinnata) 'Yamatohime' in in vitro culture. Nodular calli were successfully induced from leaf and stem explants on 30 g l −1 sucrosecontaining MS medium supplemented singly with one of the cytokinins, 6-benzyladenine (BA), zeatin or thidiazuron (TDZ) and the highest frequency of nodular callus induction was obtained on medium containing 10 mg l −1 TDZ. The nodular callus induced on this medium subsequently produced a tissue with numerous shoot primordial structures called 'mass of shoot primordia' (MSP), which was maintained at least for more than 2 years on the same medium. Shoot induction occurred after transfer the MSP onto hormone-free MS medium, in which replacement of sucrose by maltose was necessary. The shoots excised from the MSP were rooted on hormone-free MS medium and successfully grown under greenhouse conditions. The regenerated plants showed the same morphological characteristics to those of the control plants and flowered normally.

The effect of spraying with amino acids and iron on some characteristics of vegetative and flowering growth of petunia

“…In addition, iron has a key role in the formation of chlorophyll through the participation of iron in the condensation of succinic acid and glycine to form Y-aminolevulinic acid, which in turn condenses to form pyrrole groups, which also condense to form protoporphyrin. This substance is for the formation of chlorophyll, stimulated by iron, 40,41 and this may be due to the effect of iron in increasing vegetative growth and increasing the number of leaves, and the importance of iron in the vital processes within the plant that accelerate the transformation of some vegetative buds to flower 42,43 and then increase Flower buds and then to an increase in the flowers number 9,44 .…”