Highly efficlent plant regeneration from mesophyll protoplasts of Indian field cultivars of tomato (Lycopersicon esculentum)

Patil, R. S.; Davey, M. R.; Power, J. B.

doi:10.1007/bf00037728

Cited by 5 publications

(3 citation statements)

References 11 publications

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“…However, the callus tissue was not organogenic. This confirmed the existing differences in the morphogenetic potential observed among genotypes, as previously discussed (Tan et al, 1987;Patil et al, 1994).…”

supporting

confidence: 90%

Professor E. Pojnar’s pioneer studies on isolated protoplasts, their continuation and development. In memory of Professor Edward Pojnar (1919-2011)

Pindel¹,

Wiszniewska²,

Piwowarczyk³

2012

bta

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This paper is dedicated to the memory of Professor Edward Pojnar (1919-2011), Head of the Department of Botany (1974-1990) at the University of Agriculture in Krakow, who initiated research on isolated plant protoplasts in Poland. Studying in Professor E.C. Cocking's Plant Physiology Laboratory at the University of Nottingham, Professor Pojnar became acquainted with the enzymatic method procedure for protoplast isolation. His postdoctoral thesis was published in 1969 as the first paper on protoplast isolation and culture procedures in Poland. The present paper is a survey of Professor Pojnar's research, as well as further studies inspired by his pioneering achievements. Recent research into protoplast technology, focused on the structural changes involved in protoplast regeneration, is also discussed.

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supporting

confidence: 90%

Professor E. Pojnar’s pioneer studies on isolated protoplasts, their continuation and development. In memory of Professor Edward Pojnar (1919-2011)

Pindel¹,

Wiszniewska²,

Piwowarczyk³

2012

bta

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“…Callus was established from explants of young leaves of LeGUS20 on Murashige and Skoog medium (Murashige and Skoog, 1962), containing 0.8% agar, 2,4-dichlorophenoxyacetic acid (2 mg mL 21 ), 6-benzylaminopurine (0.5 mg mL 21 ), and kanamycin (80 mg mL 21 ) at 26°C under low light (150 mmol m 22 s 21 ) for 16 h light and an 8-h dark photoperiod, and subcultured at intervals of 2 to 3 weeks. Tomato cell suspension cultures were initiated from callus, as described by Patil et al (1994), in the same medium (as callus) but lacking agar and kanamycin. It was maintained by dilution (1:5) every 2 weeks and incubated at 25°C and with shaking at 120 rpm in the dark.…”

Section: Plasmid Constructionmentioning

confidence: 99%

The alc-GR System. A Modified alc Gene Switch Designed for Use in Plant Tissue Culture

et al. 2005

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The ALCR/alcA (alc) two-component, ethanol-inducible gene expression system provides stringent control of transgene expression in genetically modified plants. ALCR is an ethanol-activated transcription factor that can drive expression from the ALCR-responsive promoter (alcA). However, the alc system has been shown to have constitutive expression when used in plant callus or cell suspension cultures, possibly resulting from endogenous inducer produced in response to lowered oxygen availability. To widen the use of the alc system in plant cell culture conditions, the receptor domain of the rat glucocorticoid receptor (GR) was translationally fused to the C terminus of ALCR to produce ALCR-GR, which forms the basis of a glucocorticoid-inducible system (alc-GR). The alc-GR switch system was tested in tobacco (Nicotiana tabacum) Bright Yellow-2 suspension cells using a constitutively expressed ALCR-GR with four alternative alcA promoter-driven reporter genes: β-glucuronidase, endoplasmic reticulum-targeted green fluorescent protein, haemagglutinin, and green fluorescent protein-tagged Arabidopsis (Arabidopsis thaliana) Arath;CDKA;1 cyclin-dependent kinase. Gene expression was shown to be stringently dependent on the synthetic glucocorticoid dexamethasone and, in cell suspensions, no longer required ethanol for induction. Thus, the alc-GR system allows tight control of alcA-driven genes in cell culture and complements the conventional ethanol switch used in whole plants.

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“…Regeneration of putative shoot buds from other transformation treatments was 19.92%-31.5% (data not shown). Patil et al (1994) found plant age and explant pre-conditioning period to be significant factors that influenced their tomato transformation experiments. The enhanced transformation rate because of preconditioning of explants was attributable to exposure of additional surface area as a result of plant cell division for integration of T-DNA from Agrobacterium into plant genome and accumulation of phenolic substances that activate virulence genes in A. tumefaciens (Lipp Joao and Brown 1993).…”

Section: Transformationmentioning

confidence: 96%

Transgenic Chili PossessingBaculovirus Chitinase GeneExhibitsin vitroFungal Inhibition

Mythili

Rashmi

Suneetha

et al. 2015

Journal of Crop Improvement

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Cultivation of chili ( Capsicum annuum L.) is limited by several fungal diseases, the serious among them being anthracnose.In the absence of a source of resistance for anthracnose in C. annuum background, the transgenic approach, i.e., introduction of a gene encoding pathogen cell wall-degrading enzyme, viz., chitinase, into the host plant against the test fungus Alternaria alternata as well as anthracnose ( Colletotrichum capsici) was explored. Chili being recalcitrant to in vitro regeneration, the regeneration protocol was optimized in cotyledonary explants using different cytokinins, viz., 6-benzyl amino purine (BAP), thidiazuron (TDZ), or zeatin, in combination with gibberellic acid (GA 3 ) and/or auxins, viz., indole-3-acetic acid (IAA) or phenyl acetic acid (PAA). Agrobacterium-mediated transformation of cotyledonary explants with Autographa californica baculovirus chitinase gene under the control of 35S promoter resulted in seven putative transformants under kanamycin selection. The Color versions of one or more of the figures in the article can be found online at www. tandfonline.com/wcim. 159 160 J. B. Mythili et al.presence of transgene was confirmed in two of the seven primary transformants. One of the transformants exhibited enhanced inhibition of the fungus Alternaria alternata in in vitro bio-assays at T0 (primary transformant) stage. The two transgenic plants were then advanced to T1 generation (progenies of T0) and their fruits were examined for resistance to anthracnose. A few lines among the progenies of each of the primary transformants exhibited higher tolerance to anthracnose than the rest of the lines and untransformed control. Higher endochitinase activity in one of the primary transformants was correlated to better expression of anthracnose tolerance in its progenies, suggesting the possibility of chitinase gene being used for developing transgenic lines tolerant to anthracnose.

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Highly efficlent plant regeneration from mesophyll protoplasts of Indian field cultivars of tomato (Lycopersicon esculentum)

Cited by 5 publications

References 11 publications

Professor E. Pojnar’s pioneer studies on isolated protoplasts, their continuation and development. In memory of Professor Edward Pojnar (1919-2011)

Professor E. Pojnar’s pioneer studies on isolated protoplasts, their continuation and development. In memory of Professor Edward Pojnar (1919-2011)

The alc-GR System. A Modified alc Gene Switch Designed for Use in Plant Tissue Culture

Transgenic Chili PossessingBaculovirus Chitinase GeneExhibitsin vitroFungal Inhibition

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