Highly informative nature of inter simple sequence repeat (ISSR) sequences amplified using tri- and tetra-nucleotide primers from DNA of cauliflower (Brassicaoleraceavar.botrytisL.)

Bornet, B.; Muller, Claire; Paulus, François; Branchard, M.

doi:10.1139/g02-061

Cited by 67 publications

(40 citation statements)

References 32 publications

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“…We successfully adapted the ISSR method for analyzing genetic material of S. purpurea by optimizing the annealing temperature for each primer. Our results support the notion that microsatellite regions are subject to evolutionary change, leading to higher polymorphism (Bornet et al, 2002;Hu et al, 2011).…”

Section: Discussionsupporting

confidence: 89%

“…The main advantages of ISSR include its relative simplicity and high reproducibility of results (Bornet and Branchard, 2001;Ning et al, 2007). Another important factor is the presumption that microsatellite markers can be linked to coding regions (Zietkiewicz et al, 1994;Bornet et al, 2002). ISSR markers are highly polymorphic, which makes them a useful tool in studies of genetic diversity.…”

Section: Introductionmentioning

confidence: 99%

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Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Sulima¹,

Przyborowski²

2013

Acta Biologica Cracoviensia Series Botanica

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This study used ISSR markers to assess the genetic diversity of a collection of 15 genotypes of Salix purpurea and 6 interspecific hybrids, employing 40 of 60 tested ISSR primers generating polymorphic amplification products. The PCR-ISSR method was adapted for S. purpurea by optimizing the annealing temperature for each primer. The polymorphism index of ISSR amplification products was 91.8% for all studied genotypes and 70.4% for S. purpurea genotypes. Nei's genetic identity statistics ranged from 0.538 to 0.958. Nei's genetic distance values were used to build a dendrogram (UPGMA) for the investigated genotypes. The dendrogram shows five clusters, and principal coordinate analysis yielded nearly the same genetic relationships among the studied genotypes. The results confirm the usefulness of ISSR markers for determining genetic diversity in S. purpurea.

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Section: Discussionsupporting

confidence: 89%

Section: Introductionmentioning

confidence: 99%

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Sulima¹,

Przyborowski²

2013

Acta Biologica Cracoviensia Series Botanica

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“…It involves amplification of the DNA segment present at an amplifiable distance between 2 identical microsatellite repeat regions oriented in opposite directions (Zietkiewicz et al, 1994). Therefore, ISSR has been widely used for varietal fingerprinting or genetic diversity analysis (Bornet et al, 2002). The dominant marker RAPD and the co-dominant marker ISSR sample multiple loci from across different genomes and help to resolve phylogenetic relationships.…”

Section: Introductionmentioning

confidence: 99%

Molecular variability of plantain ecotypes from the genus Musa (Musaceae)

Choudhary¹,

Keshavachandran²,

Menon³

et al. 2014

Turk J Bot

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IntroductionBanana (Musa L. species (Musaceae)) is considered as an important food-fruit crop and is cultivated commercially in more than 120 countries. India is a major banana producing nation with an area of 0.79 million hectares and productivity of 28.4 metric tonnes per hectare (NHB, 2011). A wide range of diploids, triploids (2n = 3x = 33), and tetraploid cultivars/landraces of banana are known that have evolved from interspecific hybrids of the 2 wild diploid species, Musa acuminate Colla (contributing ' A' genome) and Musa balbisiana Colla (contributing 'B' genome). Despite botanical homogeneity, plantains manifest wide and unique phenotypic variability regarding plant size, bunch type, bunch and fruit orientation, fruit apex shape, pseudostem, and fruit color. Nendran (Musa AAB group) is one of the leading banana cultivars belonging to the plantain subgroup and is one of the most prized plantain varieties of India. However, biodiversity in the Nendran cultivar is complex and is represented by clones distinguishable by variation in plant stature, bunch, fruit morphology, and degree of development of the male phase (Menon et al., 2002). Therefore, the present study was carried out with the objective to characterize variability in plantain ecotypes of banana using molecular markers. The development and application of molecular markers provide powerful tools to reveal polymorphism, are robust to detect genetic variability (Simmons et al., 2007), and are not influenced by environment or developmental stages of the plant, thus making them an ideal tool for genetic relationship studies. However, the potential usefulness of Abstract: Twelve plantain ecotypes were characterized for molecular variability using random amplified polymorphic DNA (RAPD) and inter simple sequence repeat (ISSR) markers. Out of the 60 RAPD and 40 ISSR primers tested, 16 RAPD and 14 ISSR primers were found polymorphic, and were used for molecular profiling of the plantain ecotypes. The average number of bands per primer, average number of polymorphic bands per primer, mean polymorphic information content (PIC) value, resolving power (Rp), and marker indices (MI) for RAPD primers were 8.63, 3.25, 0.87, 15.49, and 2.86, whereas the respective values for the ISSR assay were 7.93, 4.14, 0.85, 12.49, and 3.55. The genetic similarity coefficient was calculated using the Jaccard coefficient. The unweighted pair group method with arithmetic averages (UPGMA)-based clustering pattern remained more or less similar for RAPD, ISSR, and combined RAPD and ISSR data. Clustering was strongly supported by high bootstrap values. The genotypes Njockkon and Changalikodan (similarity coefficient > 0.94) and Manjeri Nendran (a) and Manjeri Nendran (b) (similarity coefficient > 0.89) were more closely related than the other ecotypes. The principal component analysis (PCA) showed results similar to those of the dendrogram. The results revealed that ISSR would be a better tool than RAPD for evaluation of genetic diversity in plantain ecotypes. Further, huge variab...

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“…They are also highly polymorphic and their use is cost effective, requiring no prior information of the sequence (Bornet et al 2002). In cereals, ISSR markers have been used to study genetic diversity and phylogenetic relationships (Kantety et al 1995;Nagaoka and Ogihara 1997;Pejic et al 1998;Blair et al 1999;Joshi et al 2000;Qian et al 2001;Matos et al 2001;Fernández et al 2002), for gene mapping (Kojima et al 1998), for gene tagging in molecular assisted selection (Akagi et al 1996;Kaushik et al 2003), and for DNA fingerprinting (Carvalho et al 2005).…”

Section: Introductionmentioning

confidence: 99%

Morphological, yield, cytological and molecular characterization of a bread wheat X tritordeum F1 hybrid

Lima-Brito

Carvalho

Martín

et al. 2006

J Genet

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The morphological, yield, cytological and molecular characteristics of bread wheat × tritordeum F 1 hybrids (2n = 6x = 42; AABBDH ch ) and their parents were analysed. Morphologically, these hybrids resembled the wheat parent. They were slightly bigger than both parents, had more spikelets per spike, and tillered more profusely. The hybrids are self-fertile but a reduction of average values of yield parameters was observed. For the cytological approach we used a double-target fluorescence in situ hybridization performed with total genomic DNA from Hordeum chilense L. and the ribosomal sequence pTa71. This technique allowed us to confirm the hybrid nature and to analyse chromosome pairing in this material. Our results showed that the expected complete homologous pairing (14 bivalents plus 14 univalents) was only observed in 9.59% of the pollen mother cells (PMCs) analysed. Some PMCs presented autosyndetic pairing of H ch and A, B or D chromosomes. The average number of univalents was higher in the wheat genome (6.8) than in the H ch genome (5.4). The maximum number of univalents per PMC was 20. We only observed wheat multivalents (one per PMC) but the frequency of trivalents (0.08) was higher than that of quadrivalents (0.058). We amplified 50 RAPD bands polymorphic between the F 1 hybrid and one of its parents, and 31 ISSR polymorphic bands. Both sets of markers proved to be reliable for DNA fingerprinting. The complementary use of morphological and yield analysis, molecular cytogenetic techniques and molecular markers allowed a more accurate evaluation and characterization of the hybrids analysed here.

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Highly informative nature of inter simple sequence repeat (ISSR) sequences amplified using tri- and tetra-nucleotide primers from DNA of cauliflower (Brassicaoleraceavar.botrytisL.)

Cited by 67 publications

References 32 publications

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Genetic Diversity of Salix Purpurea L. Genotypes and Interspecific Hybrids

Molecular variability of plantain ecotypes from the genus Musa (Musaceae)

Morphological, yield, cytological and molecular characterization of a bread wheat X tritordeum F1 hybrid

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