Highly parallel integrated microfluidic biochannel arrays

Liu, R.H.; Chen, H.; Luehrsen, Kenneth R.; Ganser, Dale; Weston, D.; Blackwell, J.; Grodzinski, Piotr

doi:10.1109/memsys.2001.906572

Cited by 1 publication

(1 citation statement)

References 5 publications

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“…Other than spotting, the probe creation has also been achieved using a microfluidic method. For instance, Liu et al designed a glass chip containing four line arrays of oligonuclecotide probes, and parallel hybridizations of human genomic DNA targets were completed by using a second chip consisting of four sample microfluidic channels . Furthermore, Lee et al created a 3 × 3 array on a gold substrate and conducted microfluidic hybridizations to detect RNA fragments derived from a transgenic plant, Arabidopsis thaliana .…”

Section: Introductionmentioning

confidence: 99%

Flexible Microarray Construction and Fast DNA Hybridization Conducted on a Microfluidic Chip for Greenhouse Plant Fungal Pathogen Detection

Wang

2007

J. Agric. Food Chem.

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This study employed a microfluidic method in which probe creation does not require pin-spotting and fast hybridization is conducted on the same microarray chip for the detection of three greenhouse pathogens ( Botrytis cinerea, Didymella bryoniae, and Botrytis squamosa). In this method, 16 oligonucleotide probe line arrays were created on a glass substrate by a microfluidic printing method. Then, low amounts of the DNA samples (1 fmol of oligonucelotides or 1.4 ng of PCR products) were introduced into the microchannels that were orthogonal to these probe lines. The hybridizations of 16 samples (21-mer complementary oligonuleotides and approximately 260 bp PCR products) were fulfilled at the channel-probe line intersections and in a short time (minutes). The optimization of probe immobilization and sample hybridization are described in detail. The method successfully detected and discriminated between two 260 bp PCR products with a one-base-pair difference from closely related greenhouse plant fungal pathogens (B. cinerea and B. squamosa).

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Section: Introductionmentioning

confidence: 99%

Flexible Microarray Construction and Fast DNA Hybridization Conducted on a Microfluidic Chip for Greenhouse Plant Fungal Pathogen Detection

Wang

2007

J. Agric. Food Chem.

View full text Add to dashboard Cite

show abstract

Highly parallel integrated microfluidic biochannel arrays

Cited by 1 publication

References 5 publications

Flexible Microarray Construction and Fast DNA Hybridization Conducted on a Microfluidic Chip for Greenhouse Plant Fungal Pathogen Detection

Flexible Microarray Construction and Fast DNA Hybridization Conducted on a Microfluidic Chip for Greenhouse Plant Fungal Pathogen Detection

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