Highly PEGylated silica nanoparticles: “ready to use” stealth functional nanocarriers

Rio-Echevarria, Iria M.; Secci, Francesco; Segat, Daniela; Guarino, Gaetano; Tavano, Regina; Causin, Valerio; Reddi, Elena; Papini, Emanuele; Mancin, Fabrizio

doi:10.1039/b921735e

Cited by 56 publications

(47 citation statements)

References 52 publications

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“…administration. In the lung, the nanocapsules remained primarily in the lung lining fluid avoiding phagocytosis by alveolar macrophages, a behaviour which was attributed to the pegylated ('stealth') surface chemistry and the small particle size, which reduce macrophage detection and phagocytosis [36][37][38], whilst promoting translocation across the air-blood barrier [29,30,39]. The increase in signal in the liver at t=24 h post-administration (absent in the 111 In-DTPA groups) suggests that at least a fraction of the lung dose was able to cross the air-blood barrier and enter into the systemic circulation as intact nanoparticles, accumulating subsequently in the liver [29,30].…”

Section: Discussionmentioning

confidence: 99%

Lung inflammation does not affect the clearance kinetics of lipid nanocapsules following pulmonary administration

Patel

Woods

Riffo‐Vasquez

et al. 2016

Journal of Controlled Release

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Lipid nanocapsules (LNCs) are semi-rigid spherical capsules with a triglyceride core that present a promising formulation option for the pulmonary delivery of drugs with poor aqueous solubility. Whilst the biodistribution of LNCs of different size has been studied following intravenous administration, the fate of LNCs following pulmonary delivery has not been reported. We investigated quantitatively whether lung inflammation affects the clearance of 50 nm lipid nanocapsules, or is exacerbated by their pulmonary administration. Studies were conducted in mice with lipopolysaccharide-induced lung inflammation compared to healthy controls. Particle deposition and nanocapsule clearance kinetics were measured by single photon emission computed tomography/computed tomography (SPECT/CT) imaging over 48 h. A significantly lower lung dose of 111 In-LNC50 was achieved in the lipopolysaccharide (LPS)-treated animals compared with healthy controls (p < 0.001). When normalised to the delivered lung dose, the clearance kinetics of 111 In-LNC50 from the lungs fit a first order model with an elimination half-life of 10.5 ± 0.9 h (R 2 = 0.995) and 10.6 ± 0.3 h (R 2 = 1.000) for healthy and inflamed lungs respectively (n = 3). In contrast, 111 In-diethylene triamine pentaacetic acid (DTPA), a small hydrophilic molecule, was cleared rapidly from the lungs with the majority of the dose absorbed within 20 min of administration. Biodistribution to lungs, stomach-intestine, liver, trachea-throat and blood at the end of the imaging period was unaltered by lung inflammation. This study demonstrated that lung clearance and whole body distribution of lipid nanocapsules were unaffected by the presence of acute lung inflammation.

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Section: Discussionmentioning

confidence: 99%

Lung inflammation does not affect the clearance kinetics of lipid nanocapsules following pulmonary administration

Patel

Woods

Riffo‐Vasquez

et al. 2016

Journal of Controlled Release

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“…[12] Unter Verwendung einer ähnlichen Strategie entwickelten Mancin und Mitarbeiter kürzlich unterschiedlich PEGylierte DDSNs (d = 20, 50, 100 nm), die ein Alkoxysilanderivat des Cyaninfarbstoffs IR775 enthielten. [33] Das gemeinsame Merkmal all dieser Syntheseverfahren ist die Notwendigkeit, eine bestimmte Menge (üblicherweise 1 Mol-% bezogen auf TEOS) des für den Erhalt eines fluoreszierenden Materials erforderlichen Fluorophors zuzugeben. Die Wahl des Verfahrens hängt demzufolge im Wesentlichen von der Löslichkeit des Fluorophors selbst ab; zum Beispiel ist die Mikroemulsion bevorzugt, wenn die emittierende Spezies sehr gut wasserlöslich ist.…”

Section: Synthese Von Funktionalisierten Siliciumdioxidnanopartikelnunclassified

Glänzende Aussichten für lumineszierende Siliciumdioxidnanopartikel

et al. 2011

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Siliciumdioxidnanopartikel verfügen über besondere Eigenschaften, z. B. eine geringe Toxizität, und stellen eine vielseitige Plattform für die Nanotechnologie dar. Ein geeignetes Design oder eine geeignete Derivatisierung führen zu selbst unter physiologischen Bedingungen stabilen Kolloiden und ermöglichen verschiedenste Funktionen. Insbesondere bei der Wahl passender Farbstoffe und Synthesestrategien können sehr hell leuchtende Nanosysteme resultieren. So bieten Siliciumdioxidnanopartikel ein einzigartiges Potenzial, und weitere Verbesserungen könnten neue Anwendungen in Bereichen wie medizinischer Diagnose und Therapie, Umwelt‐ und Lebensmittelanalytik sowie Sicherheit bringen. Hier stellen wir Mehrkomponenten‐Nanosysteme auf Siliciumdioxidbasis vor, die zu gerichtetem Energie‐ und Elektronentransfer fähig sind und sich für Funktionen wie Lichtsammeln und Signalverstärkung eignen.

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“…SiO 2 –NPs nanoparticles are also widely studied, as they can be employed as innovative therapeutic agents because of their versatility and low‐cost synthesis. However, because SiO 2 –NPs are not biocompatible, they must be functionalized to confer them stealth properties, in order to avoid clearance …”

Section: Introductionmentioning

confidence: 99%

XRF mapping and TEM analysis of coated and uncoated silica nanoparticles in A549 cells and human monocytes

et al. 2019

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The evaluation of nanomaterials intracellular distribution still remains a challenge in nanomedicine applications and toxicological studies. Synchrotron radiation X‐ray microscopy combined with X‐ray fluorescence (XRF) microspectroscopy provides unique information that has pushed the frontiers of biological research, particularly when investigating intracellular mechanisms. In this work, the presence of silica nanoparticles in in vitro cultured human lung epithelial cell line and freshly extract human monocytes has been investigated. For the uptake and intracellular distribution of NPs, cells were cultured on polymeric substrates (Mylar). The SiO2–NPs have been synthesized at JRC and characterized by dynamic light scattering, centrifugal liquid sedimentation, and transmission electron microscopy (TEM), whereas their interaction with cells was investigated with TEM and XRF. For the latter, we used TwinMic in scanning transmission mode coupled with low‐energy XRF spectroscopy, paying particular attention to the distribution of different elements, namely, Fe, O, C, Si, and Mg. Si XRF signals recorded on cells exposed to uncoated silica and epoxy‐coated silica nanoparticles are comparable, indicating low difference in cellular uptake and suggesting a similar interaction between nanoparticles and cells. However, the TEM analysis indicates a better affinity of the coated nanoparticles for the cell membrane. Moreover, the TEM analysis shows also the presence of nanoparticles in endosomes.

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Highly PEGylated silica nanoparticles: “ready to use” stealth functional nanocarriers

Cited by 56 publications

References 52 publications

Lung inflammation does not affect the clearance kinetics of lipid nanocapsules following pulmonary administration

Lung inflammation does not affect the clearance kinetics of lipid nanocapsules following pulmonary administration

Glänzende Aussichten für lumineszierende Siliciumdioxidnanopartikel

XRF mapping and TEM analysis of coated and uncoated silica nanoparticles in A549 cells and human monocytes

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