2021
DOI: 10.1021/acs.bioconjchem.1c00309
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Highly Photoluminescent Nitrogen- and Zinc-Doped Carbon Dots for Efficient Delivery of CRISPR/Cas9 and mRNA

Abstract: Safe and efficient delivery of CRISPR/Cas9 systems is still a challenge. Here we report the development of fluorescent nitrogen- and zinc-doped carbon dots (N–Zn-doped CDs) using one-step microwave-aided pyrolysis based on citric acid, branched PEI25k, and different zinc salts. These versatile nanovectors with a quantum yield of around 60% could not only transfect large CRISPR plasmids (∼9 kb) with higher efficiency (80%) compared to PEI25k and lipofectamine 2000 (Lipo 2K), but they also delivered mRNA into HE… Show more

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Cited by 21 publications
(22 citation statements)
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“…In these examples, the co-assembly allowed to tune the CDs emissive properties through interaction of negatively charged nanoparticles with cationic supramolecular polymers [80] and endowed positively charged CDs with circularly polarized luminescence by bonding with anionic chiral peptide hydrogelators. [81] Some CDs can self-assemble through electrostatic interactions, with the resulting so-called "supra-CDs" featuring absorption into the visible to near-infrared (NIR) range, which [44] b) in photo(bio) redox catalysis, [45] c) for interaction with covalent and supramolecular polymers, [46] d) in self-aggregation for photothermal therapy, [47,48] e) for gene delivery, [49][50][51] and f) for interfacing with proteins and cells. [52,53] can be exploited for photothermal therapy (Figure 3d).…”
Section: Electrostatic Interactionsmentioning
confidence: 99%
“…In these examples, the co-assembly allowed to tune the CDs emissive properties through interaction of negatively charged nanoparticles with cationic supramolecular polymers [80] and endowed positively charged CDs with circularly polarized luminescence by bonding with anionic chiral peptide hydrogelators. [81] Some CDs can self-assemble through electrostatic interactions, with the resulting so-called "supra-CDs" featuring absorption into the visible to near-infrared (NIR) range, which [44] b) in photo(bio) redox catalysis, [45] c) for interaction with covalent and supramolecular polymers, [46] d) in self-aggregation for photothermal therapy, [47,48] e) for gene delivery, [49][50][51] and f) for interfacing with proteins and cells. [52,53] can be exploited for photothermal therapy (Figure 3d).…”
Section: Electrostatic Interactionsmentioning
confidence: 99%
“…Cas9/sgRNA plasmid CDK11 gene MCF-7 cells NHEJ [167] Protamine/gold nanoclusters Cas9/sgRNA plasmid E7 U2OS cells NHEJ [168] Carbon dots Cas9/sgRNA plasmid GFP HEK 293T cells NHEJ [169] Semiconducting polymer/PEI/PEG/alkyl side chains/Dexamethasone hybrid NPs Cas9/sgRNA plasmid GFP HCCT 116 cells-subcutaneous tumor NHEJ [124] pCas9-loaded CLANs was similar to Lipofectamine 2000 and induced 46.8% indel of BCR-ABL gene in K562 cells after three days. However, the cell toxicity caused by this CLAN is much lower than that of Lipofectamine 2000.…”
Section: Genome Editing Referencementioning
confidence: 99%
“…Lanthanide-doped hollow NPs (LDHNs), as gene delivery vehicles, protected the pDNA (containing enhanced GFP) and increased the transfection efficiency [ 191 ]. Fluorescent nitrogen- and zinc-doped CDs (N–Zn-doped CDs) showed excellent transfection efficiency and safe delivery of clustered regularly interspaced short palindromic repeats/Cas9 (CRISPR/Cas9) complexes and mRNA (for degradation of GFP) in HEK 293 T-GFP cells (cells from a kidney human embryo) [ 192 ].…”
Section: Application Of Various Mnps For Nucleic Acid Deliverymentioning
confidence: 99%