Highly porous polycaprolactone scaffolds doped with calcium silicate and dicalcium phosphate dihydrate designed for bone regeneration

Gandolfi, Maria Giovanna; Zamparini, Fausto; Esposti, Mauro Degli; Chiellini, Emo; Fava, Fabio; Fabbri, Paola; Taddei, Paola; Prati, Carlo

doi:10.1016/j.msec.2019.04.040

Cited by 50 publications

(51 citation statements)

References 89 publications

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“…Mineral doped scaffolds, composed of a biodegradable synthetic polymer and bioactive mineral fillers showed attractive properties for their use in bone tissue engineering [13,30]. Biodegradable synthetic polymers such as those based on poly-α-hydroxyl acids (as PLA and PCL) are currently used for scaffold design, as they are biocompatible, biodegradable, with an appropriate shelf-life, may be produced with a low cost process and may be adapted to the surgical site [13,15].…”

Section: Discussionmentioning

confidence: 99%

“…The scaffolds were prepared through TIPS according to a previous protocol [28][29][30]. PLA or PCL solutions in 1,4-dioxane were obtained, reaching a 3.5% wt/vol concentration.…”

Section: Scaffolds Preparationmentioning

confidence: 99%

“…CaSi and DCPD mineral powders were added in 10% by weight with respect to PCL or PLA. The solutions were sonicated for 3 h using a ultrasonic processor UP50H (Hielsher, Teltow, Germany; operative parameters 50 W, 30 kHz) with a 2 mm sonotrode MSD titanium tip, then put inside 60 mm plates and cooled at −18 • C for 18 h. Finally, the frozen samples were extracted and immersed in an ethanol bath (Sigma-Aldrich, Milan, Italy) precooled at −18 • C for 18 h, with a solvent refresh every 3 h [29,30].…”

Section: Scaffolds Preparationmentioning

confidence: 99%

“…have been designed for angiogenic and osteogenic purposes containing bioactive calcium silicates [31]. Recently, highly porous PLA and PCL scaffolds doped with bioactive calcium silicates and calcium phosphates have been designed [28][29][30]. Mineral-doped PLA scaffolds demonstrated the ability to be colonized by oral derived mesenchymal stem cells and to stimulate their shift through osteogenic lineage [28].…”

mentioning

confidence: 99%

“…Mineral-doped PLA scaffolds demonstrated the ability to be colonized by oral derived mesenchymal stem cells and to stimulate their shift through osteogenic lineage [28]. Highly porous mineral-doped PCL scaffolds showed interesting biointeractive properties and the ability to nucleate apatite, enhancing the biological properties of pure PCL [30].…”

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confidence: 99%

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Vascular Wall–Mesenchymal Stem Cells Differentiation on 3D Biodegradable Highly Porous CaSi-DCPD Doped Poly (α-hydroxy) Acids Scaffolds for Bone Regeneration

et al. 2020

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Vascularization is a crucial factor when approaching any engineered tissue. Vascular wall-mesenchymal stem cells are an excellent in vitro model to study vascular remodeling due to their strong angiogenic attitude. This study aimed to demonstrate the angiogenic potential of experimental highly porous scaffolds based on polylactic acid (PLA) or poly-e-caprolactone (PCL) doped with calcium silicates (CaSi) and dicalcium phosphate dihydrate (DCPD), namely PLA-10CaSi-10DCPD and PCL-10CaSi-10DCPD, designed for the regeneration of bone defects. Vascular wall-mesenchymal stem cells (VW-MSCs) derived from pig thoracic aorta were seeded on the scaffolds and the expression of angiogenic markers, i.e. CD90 (mesenchymal stem/stromal cell surface marker), pericyte genes α-SMA (alpha smooth muscle actin), PDGFR-β (platelet-derived growth factor receptor-β), and NG2 (neuron-glial antigen 2) was evaluated. Pure PLA and pure PCL scaffolds and cell culture plastic were used as controls (3D in vitro model vs. 2D in vitro model). The results clearly demonstrated that the vascular wall mesenchymal cells colonized the scaffolds and were metabolically active. Cells, grown in these 3D systems, showed the typical gene expression profile they have in control 2D culture, although with some main quantitative differences. DNA staining and immunofluorescence assay for alpha-tubulin confirmed a cellular presence on both scaffolds. However, VW-MSCs cultured on PLA-10CaSi-10DCPD showed an individual cells growth, whilst on PCL-10CaSi-10DCPD scaffolds VW-MSCs grew in spherical clusters. In conclusion, vascular wall mesenchymal stem cells demonstrated the ability to colonize PLA and PCL scaffolds doped with CaSi-DCPD for new vessels formation and a potential for tissue regeneration.

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Section: Discussionmentioning

confidence: 99%

“…The scaffolds were prepared through TIPS according to a previous protocol [28][29][30]. PLA or PCL solutions in 1,4-dioxane were obtained, reaching a 3.5% wt/vol concentration.…”

Section: Scaffolds Preparationmentioning

confidence: 99%

Section: Scaffolds Preparationmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Vascular Wall–Mesenchymal Stem Cells Differentiation on 3D Biodegradable Highly Porous CaSi-DCPD Doped Poly (α-hydroxy) Acids Scaffolds for Bone Regeneration

et al. 2020

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Research Progress of Shape Memory Polymer and 4D Printing in Biomedical Application

Zhao

Yue

Liu

et al. 2022

Adv Healthcare Materials

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As a kind of smart material, shape memory polymer (SMP) shows great application potential in the biomedical field. Compared with traditional metal-based medical devices, SMP-based devices have the following characteristics: 1) The adaptive ability allows the biomedical device to better match the surrounding tissue after being implanted into the body by minimally invasive implantation; 2) it has better biocompatibility and adjustable biodegradability; 3) mechanical properties can be regulated in a large range to better match with the surrounding tissue. 4D printing technology is a comprehensive technology based on smart materials and 3D printing, which has great application value in the biomedical field. 4D printing technology breaks through the technical bottleneck of personalized customization and provides a new opportunity for the further development of the biomedical field. This paper summarizes the application of SMP and 4D printing technology in the field of bone tissue scaffolds, tracheal scaffolds, and drug release, etc. Moreover, this paper analyzes the existing problems and prospects, hoping to provide a preliminary discussion and useful reference for the application of SMP in biomedical engineering.

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Engineering of Bioresorbable Polymers for Tissue Engineering and Drug Delivery Applications

Dobrzyńska‐Mizera,

Dodda,

Liu

et al. 2024

Adv Healthcare Materials

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Herein, the recent advances in the development of resorbable polymeric‐based biomaterials, their geometrical forms, resorption mechanisms, and their capabilities in various biomedical applications are critically reviewed. A comprehensive discussion of the engineering approaches for the fabrication of polymeric resorbable scaffolds for tissue engineering, drug delivery, surgical, cardiological, aesthetical, dental and cardiovascular applications, are also explained. Furthermore, to understand the internal structures of resorbable scaffolds, representative studies of their evaluation by medical imaging techniques, e.g., cardiac computer tomography, are succinctly highlighted. This approach provides crucial clinical insights which help to improve the materials’ suitable and viable characteristics for them to meet the highly restrictive medical requirements. Finally, the aspects of the legal regulations and the associated challenges in translating research into desirable clinical and marketable materials of polymeric‐based formulations, are presented.

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Highly porous polycaprolactone scaffolds doped with calcium silicate and dicalcium phosphate dihydrate designed for bone regeneration

Cited by 50 publications

References 89 publications

Vascular Wall–Mesenchymal Stem Cells Differentiation on 3D Biodegradable Highly Porous CaSi-DCPD Doped Poly (α-hydroxy) Acids Scaffolds for Bone Regeneration

Vascular Wall–Mesenchymal Stem Cells Differentiation on 3D Biodegradable Highly Porous CaSi-DCPD Doped Poly (α-hydroxy) Acids Scaffolds for Bone Regeneration

Research Progress of Shape Memory Polymer and 4D Printing in Biomedical Application

Engineering of Bioresorbable Polymers for Tissue Engineering and Drug Delivery Applications

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