Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach

Saleem, Saima; Sajid, Muhammad; Hussain, Dilshad; Fatima, Batool; Jabeen, Fahmida; Najam‐ul‐Haq, Muhammad; Saeed, Adeela

doi:10.1007/s00604-020-04532-z

Cited by 20 publications

(12 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, this derivatization route has no stringent pH requirements, and the use of less organic solvent appears to represent a greener strategy. When compared with existing boronic acid approaches, 30,32,33,37,38 UiO‐66@PEI@Au@B(OH) 2 still shows a range of good properties, indicating its great potential for application in glycoproteomics. The details can be found in Table 1.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Construction of boric acid‐functionalized metal–organic frameworks for glycopeptide recognition in the serum of cervical cancer patients

Rao¹,

Wang

Zhong³

et al. 2022

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

Rationale Cervical cancer is one of the most common malignant tumors in women, and it is essential to explore potential biomarkers such as glycopeptides closely related to cancer in physiological samples of cervical cancer patients. Sample pretreatment is required before direct detection using mass spectrometry because there are certain limitations. Meanwhile, it is still highly desired to promote the functionalization and application of metal–organic framework (MOF)‐derived materials. Methods Using a post‐synthesis modification method, a novel type of boric acid‐functionalized MOF probe (designated as UiO‐66@PEI@Au@B(OH)2) is prepared for recognition of glycopeptides. The results are obtained using matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometry and nano‐liquid chromarography–tandem mass spectrometry. Results The UiO‐66@PEI@Au@B(OH)2 probe exhibits a low detection limit (0.6 fmol μL−1), an excellent recovery rate, comparatively good reusability and selectivity (HRP digests:BSA digests = 1:500). When UiO‐66@PEI@Au@B(OH)2 is used to selectively capture glycopeptides from the serum of a healthy person and a cervical cancer patient, 101 glycopeptides corresponding to 54 glycoproteins and 108 glycopeptides corresponding to 57 glycoproteins are detected, respectively. Conclusions The successful preparation of UiO‐66@PEI@Au@B(OH)2 provides a path for the investigation of the functionalization of MOF‐derived materials. The excellent performance of UiO‐66@PEI@Au@B(OH)2 not only demonstrates the huge potential of functionalized MOFs in the glycoproteome, but also opens up new phases of the application of MOF‐based materials.

show abstract

Section: Resultsmentioning

confidence: 99%

“…When compared with existing boronic acid approaches, 30,32,33,37,38 UiO-66@PEI@Au@B(OH) 2 still shows a range of good properties, indicating its great potential for application in glycoproteomics. The details can be found in Table 1.…”

Section: Capture Of Glycopeptides From Serum Of Healthy Person and Ce...mentioning

confidence: 99%

Construction of boric acid‐functionalized metal–organic frameworks for glycopeptide recognition in the serum of cervical cancer patients

Rao¹,

Wang

Zhong³

et al. 2022

Rapid Comm Mass Spectrometry

View full text Add to dashboard Cite

show abstract

“…After exposing enriched and PNGase-treated deglycosylated peptides to LC-MS, 318 N-linked glycopeptides were discovered from 1 L human serum digest. As a result, terpolymer@ZIF-8@BA has the capacity to produce mono-glycosylated and multi-glycosylated peptides from complicated biological samples [ 129 ].…”

Section: Applications Of Zn-based Mofs For Gases Adsorption Imaging and Sensorsmentioning

confidence: 99%

Zinc-Based Metal-Organic Frameworks in Drug Delivery, Cell Imaging, and Sensing

Ali

Meng

2021

Molecules

View full text Add to dashboard Cite

The design and structural frameworks for targeted drug delivery of medicinal compounds and improved cell imaging have been developed with several advantages. However, metal-organic frameworks (MOFs) are supplemented tremendously for medical uses with efficient efficacy. These MOFs are considered as an absolutely new class of porous materials, extensively used in drug delivery systems, cell imaging, and detecting the analytes, especially for cancer biomarkers, due to their excellent biocompatibility, easy functionalization, high storage capacity, and excellent biodegradability. While Zn-metal centers in MOFs have been found by enhanced efficient detection and improved drug delivery, these Zn-based MOFs have appeared to be safe as elucidated by different cytotoxicity assays for targeted drug delivery. On the other hand, the MOF-based heterogeneous catalyst is durable and can regenerate multiple times without losing activity. Therefore, as functional carriers for drug delivery, cell imaging, and chemosensory, MOFs’ chemical composition and flexible porous structure allowed engineering to improve their medical formulation and functionality. This review summarizes the methodology for fabricating ultrasensitive and selective Zn-MOF-based sensors, as well as their application in early cancer diagnosis and therapy. This review also offers a systematic approach to understanding the development of MOFs as efficient drug carriers and provides new insights on their applications and limitations in utility with possible solutions.

show abstract

“…So far, boric acid chemistry [16], hydrazine chemistry, lectin affinity method [17], and hydrophilic interaction chromatography (HILIC) [18,19] have become the main enrichment methods for glycopeptides. Among them, HILIC has been extensively applied in the separation and enrichment of glycopeptides [20,21], and many HILICbased materials, including metal organic frameworks (MOFs) [22] and graphene oxide [23], have been explored to capture glycopeptides [24,25]. Although a certain degree of performance has been achieved, HILIC-based nanomaterials also have the limitation of insufficient hydrophilicity.…”

Section: Introductionmentioning

confidence: 99%

Bi‐amino acid functionalized biomimetic honeycomb chitosan membrane as a multifunctional hydrophilic probe for specific capture of N‐linked glycopeptides in nasopharyngeal carcinoma's disease patient's serum

Wang

et al. 2022

J of Separation Science

View full text Add to dashboard Cite

In this work, a novel porous bifunctionalized composite material was synthesized via a simple method. Gold nanoparticles are uniformly dispersed on the surface of the biomimetic honeycomb chitosan membrane through the interaction between amino and Au, and then cysteine and glutathione are successfully grafted onto the surface of the Au by the Au‐S bond. The modification of cysteine and glutathione makes this bifunctionalized composite material have significant advantages of superhydrophilicity and small steric hindrance simultaneously. This material manifests excellent property in glycopeptides enrichment, with high selectivity (1:5000), low detection limit (0.1 fmol·μL–1), high recovery rate (99.4 ± 0.5%), and good repeatability. In addition, with the help of nano‐flow liquid chromatography tandem mass spectrometry, this composite achieved excellent performance in efficiently enriching glycopeptides in the serum of healthy people and nasopharyngeal carcinoma's disease patient. More excitingly, further gene ontology analysis of molecular function and biological process indicated that 41 original glycoproteins of the identified glycopeptides from serum of nasopharyngeal carcinoma's disease patient significantly partake in numerous cancer‐associated events, including protease binding, calcium ion binding, enzyme binding, extracellular matrix organization, cellular response to tumor necrosis factor, and inflammatory response.

show abstract

Highly porous terpolymer-ZIF8@BA MOF composite for identification of mono- and multi-glycosylated peptides/proteins using MS-based bottom-up approach

Cited by 20 publications

References 32 publications

Construction of boric acid‐functionalized metal–organic frameworks for glycopeptide recognition in the serum of cervical cancer patients

Construction of boric acid‐functionalized metal–organic frameworks for glycopeptide recognition in the serum of cervical cancer patients

Zinc-Based Metal-Organic Frameworks in Drug Delivery, Cell Imaging, and Sensing

Bi‐amino acid functionalized biomimetic honeycomb chitosan membrane as a multifunctional hydrophilic probe for specific capture of N‐linked glycopeptides in nasopharyngeal carcinoma's disease patient's serum

Contact Info

Product

Resources

About