Highly selective and sensitive detection of Staphylococcus aureus with gold nanoparticle-based core-shell nano biosensor

Shahbazi, Reza; Salouti, Mojtaba; Amini, Bahram; Jalilvand, Ahmad; Naderlou, Ebrahim; Amini, Ali; Shams, Arash

doi:10.1016/j.mcp.2018.07.004

Cited by 46 publications

(25 citation statements)

References 25 publications

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“…It is used for environmental monitoring, detection of pathogenic bacteria and food safety [11,12]. Enzymes and gold nanoparticles are mostly used in colorimetric technique but the critical operating situation, high price and short lifetime prevent them from being used as a routine test in laboratories [13,14]. Paramagnetic nanoparticles and Silica nanoparticles with fast kinetics in solution, quick and easy separation by the magnet, excellent compatibility and ease of synthesis, low cost of production and the presence of a large hydrophilic surface, have more applicability [15].…”

Section: Introductionmentioning

confidence: 99%

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Shams

Zarif

2019

Artificial Cells, Nanomedicine, and Biotechnology

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Brucellosis has always been a threat to the health and economics of societies. We report a new colorimetric immunoassay based on colored silica nanoparticles for detection of Brucella abortus. An immunosensor was designed based on blue-SiNPs and paramagnetic nanoparticles (PMNPs). The synthesized immunosensor was conjugated with a polyclonal antibody against B. abortus, which was activated by 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC) and N-hydroxysuccinimide (NHS) to form detection and capture probes, respectively. After adding the conjugates to the bacterial suspension, sandwich structure of PMNPs B. abortus-blue-SiNPs was formed and then separated by a magnet. The blue dye was released from the silica structure and its absorbance was measured at 670 nm with a spectrophotometer. Under optimal conditions, results showed a wide dynamic range from 1.5 Â 10 3 to 1.5 Â 10 8 cfu mL À1 with a detection limit of 450 cfu mL À1. The specificity of the sensor was confirmed in comparison with 5 other bacteria. Also, during the 120-days period, the complex was stable. The results suggested that it can be used in real samples (R 2 ¼ .9865). This designed colorimetric immunoassay strategy can be used as an alternative, user-friendly and on-site tool for the rapid detection of Brucella spp. compared to other common methods with high sensitivity and specificity in a short time.

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Section: Introductionmentioning

confidence: 99%

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Shams

Zarif

2019

Artificial Cells, Nanomedicine, and Biotechnology

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“…For complete of discussion, Table 2 25‐206 summarized all of the discussed articles on the detection of bacterial. It is important to point out that, there is other reports in this research area …”

Section: Biosensing Based On Type Of Bacteriamentioning

confidence: 99%

Recent progress and challenges on the bioassay of pathogenic bacteria

2020

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The present review (containing 242 references) illustrates the importance and application of optical and electrochemical methods as well as their performance improvement using various methods for the detection of pathogenic bacteria. The application of advanced nanomaterials including hyper branched nanopolymers, carbon‐based materials and silver, gold and so on. nanoparticles for biosensing of pathogenic bacteria was also investigated. In addition, a summary of the applications of nanoparticle‐based electrochemical biosensors for the identification of pathogenic bacteria has been provided and their advantages, detriments and future development capabilities was argued. Therefore, the main focus in the present review is to investigate the role of nanomaterials in the development of biosensors for the detection of pathogenic bacteria. In addition, type of nanoparticles, analytes, methods of detection and injection, sensitivity, matrix and method of tagging are also argued in detail. As a result, we have collected electrochemical and optical biosensors designed to detect pathogenic bacteria, and argued outstanding features, research opportunities, potential and prospects for their development, according to recently published research articles.

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“…Rapid and low-cost detection technique for diagnosis of pathogen S. aureus in different environments is critical for infectious diseases control [80][81][82] Using SpA-targeting aptamers conjugated to a porous silicon (PSi) nanostructure to specifically quantify SpA in a range of 2-50 µM in less than 2 H [80] Using carboxyl graphene as the carrier of IgG to develop electrochemiluminescent (ECL) biosensor to detect S. aureus because of specific reaction between SpA and IgG molecules [83] been made to improve the alkaline stability of the IgG-binding domains of the SpA using genetic engineering methods. The C domain has been shown to be fairly resistant to alkaline conditions compared with the other domains, probably due to the presence of Thr-23 instead of the Asn-23 in other domains [78].…”

Section: Application Of Spa In Diagnostic Biosensorsmentioning

confidence: 99%

“…A color change can easily be detected by just a portable UV-visible spectrophotometer and is very useful for rapid detection of pathogens in the environment [82]. A color change can easily be detected by just a portable UV-visible spectrophotometer and is very useful for rapid detection of pathogens in the environment [82].…”

Section: Novel Application Of Spa In Diagnostic Biosensorsmentioning

confidence: 99%

A comprehensive review on staphylococcal protein A (SpA): Its production and applications

Rigi

Ghaedmohammadi

Ahmadian

2019

Biotech and App Biochem

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The Staphylococcus aureus protein A (SpA) can be obtained through the culture of wild-type S. aureus and also as a recombinant protein in safe bacterial hosts. Several methods have been used to purify SpA among which ion-exchange chromatography, affinity chromatography, gel filtration, and per aqueous liquid chromatography (PALC) are common. SpA has a wide range of biochemical, biotechnological, and medical applications and is most commonly used in test methods such as immunoprecipitation, enzyme-linked immunosorbent assay, and Western blotting. SpA has also been widely utilized in pharmaceutical applications to bind to immune complexes and serum immunoglobulins. SpA also directly binds to the B-cells preventing initiation of infectious diseases as well as having a role in the development of various autoimmune diseases. This review considers different applications of SpA in biotechnology and its novel clinical application for effective treatment of autoimmune diseases. It also discusses various strategies for expression and purification of the SpA including types of column chromatography that are commonly used in protein purification and developing SpA surface display technologies. Finally, this review highlights the potential and novel applications of SpA immobilization, SpA typing, protein engineering for further development of immunological and biochemical research, and also application of SpA as a diagnostic biosensor.

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Highly selective and sensitive detection of Staphylococcus aureus with gold nanoparticle-based core-shell nano biosensor

Cited by 46 publications

References 25 publications

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Designing an immunosensor for detection of Brucella abortus based on coloured silica nanoparticles

Recent progress and challenges on the bioassay of pathogenic bacteria

A comprehensive review on staphylococcal protein A (SpA): Its production and applications

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