Highly selective and sensitive SPR immunosensor for detection of methamphetamine

Sakai, Go; Nakata, Seiji; Uda, Takaaki; Miura, Norio; Yamazoe, Noboru

doi:10.1016/s0013-4686(99)00092-4

Cited by 81 publications

(39 citation statements)

References 18 publications

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“…13,16,17 BSA has been widely used for nonspecific binding reduction in biological detection. 21,41,42 In this study, the BSA surface tailored SPIONs was shown to exhibit superior antifouling on reduction of nonspecific cellular binding based on the cell assay results ( Figure 6). BSA has been found in this study to serve two major functionalities, namely the ligand exchange for water solubilization of SPIONs and antifouling for reduction of nonspecific cellular binding.…”

Section: In Vitro Laser Scanning Confocal and Mr Imagingmentioning

confidence: 74%

Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells

Wang¹,

Xing²,

Zhang³

et al. 2014

IJN

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Targeted imaging contrast agents for early pancreatic ductal adenocarcinoma diagnosis was developed using superparamagnetic iron oxide nanoparticles (SPIONs). For phase transfer of SPIONs, the hydrophobic SPIONs are first treated with tetrafluoroborate and then capped by bovine serum albumin (BSA) via ligand exchange. It was experimentally found that nitrosyl tetrafluoroborate pretreatment and proper structures of molecules are essential to the effective surface functionalization of SPIONs. Nonspecific binding was found to be significantly reduced by BSA surface functionalized hydrophobic SPIONs (BSA·SPIONs). The BSA·SPIONs were monodispersed with an average size of approximately 18.0 nm and stable in a wide pH range and various ionic strengths even after 7 days of storage. The longitudinal and transverse proton relaxation rate (r 1 , r 2 ) values of the BSA·SPIONs were determined to be 11.6 and 154.2 s −1 per mM of Fe 3+ respectively. The r 2 /r 1 ratio of 13.3 ensured its application as the T 2 -weighted magnetic resonance imaging contrast agents. When conjugated with near-infrared fluorescent dye and monoclonal antibody, the dye BSA·SPION-monoclonal antibody bioconjugates showed excellent targeting capability with minimal nonspecific binding in the bimodal imaging of pancreatic cancer cells. The experimental approach is facile, environmentally benign, and straightforward, which presents great promise in early cancer diagnosis.

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Section: In Vitro Laser Scanning Confocal and Mr Imagingmentioning

confidence: 74%

Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells

Wang¹,

Xing²,

Zhang³

et al. 2014

IJN

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“…Addition to enhance the absorption of biomelecule or enzyme, the SPR sensing surface will be modified by thin polymer layer [14,15]. Moreover, the covalent binding between antigen and antibody has also a powerful method for biosensors such as detection of metamphetamine, detection of low molecular weight domoic acid, medical diagnostics of human hepatitis B virus (hHBV), assay of the different poliovirus types [16][17][18][19][20]. Another important field is study an immobilization of tTGas relating to the Ca 2+ concentration, which is so important in human health [21].…”

Section: Surface Plasmon Resonance Sensors For Disease Diagnosis 2/3mentioning

confidence: 99%

Surface Plasmon Resonance Sensors for Disease Diagnosis

Nguyen¹

2017

IJBSBE

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“…Each immunoreaction-regeneration cycle took only 1000 s. were determined using a Langmuir-type adsorption mode [42][43][44][45][46]. K 2 , which is defined as the secondary affinity constant K 1 of Ab to the free clenbuterol in the solution, was also determined.…”

Section: Detection Of Clenbuterolmentioning

confidence: 99%

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

Suherman

Morita

Kawaguchi

2015

Sensors and Actuators B: Chemical

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This work aims to find the key factor determining the detection limit for an indirect competitive inhibition immunoassay using surface plasmon resonance (SPR) sensing. In our previous work, the thiol solution concentration used in a self-assembly process highly affected the alkanethiol monolayer structure on the sensor surface [Suherman et al., 2014]. It was noticed that the monolayer structure determined the immunoassay sensitivity due to the orientation and the surface concentration of antigen in domain structure of monolayer. To study the effect of orientation of antigen, we examined here three types of alkanethiol compounds: dithiobis(succinimidyl undecanoate) (DSU; straight-chain alkanethiol), carboxy-EG 6 -undecanethiol (CEG 6 ; flexible-chain alkanethiol), and(C 2 -NTA; three-branched alkanethiol). By electrochemical reductive desorption, the surface concentration of DSU, CEG 6 , and C 2 -NTA were estimated to be 6.6 × 10 -10 , 8.5 × 10 -10 , 4.6 × 10 -10 mol cm -2 , respectively. Subsequently, SPR suggested that the ratios of immobilized antigen (clenbuterol) per thiol were 0.13, 0.16, and 0.15 for DSU, CEG 6 , and C 2 -NTA, respectively. This suggests that the amount of immobilized clenbuterol was defined by the molecular size of clenbuterol. Sensor surface structures on a molecular scale were evaluated using high-resolution scanning tunneling microscopy (STM), which showed tilted clenbuterol with θ DSU > θ CEG6 > θ C2-NTA . For SPR sensing of clenbuterol, C 2 -NTA showed the highest sensitivity (LOD = 10 ppt) among the examined alkanethiols, because clenbuterol formed a laid-on structure. Based on kinetics, the key factor for sensing performance is discussed.2

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Highly selective and sensitive SPR immunosensor for detection of methamphetamine

Cited by 81 publications

References 18 publications

Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells

Surface engineered antifouling optomagnetic SPIONs for bimodal targeted imaging of pancreatic cancer cells

Surface Plasmon Resonance Sensors for Disease Diagnosis

Effect of alkanethiol molecular structure on sensitivity of surface plasmon resonance sensor

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