Highly sensitive aflatoxin B1 sensor based on DNA-guided assembly of fluorescent probe and TdT-assisted DNA polymerization

Wang, Bin; Zheng, Jiushang; Ding, Ailing; Xu, Liqun; Chen, Jiuncun; Li, Chang Ming

doi:10.1016/j.foodchem.2019.05.025

Cited by 25 publications

(22 citation statements)

References 24 publications

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“…The effectiveness of aptasensors using fluorescent-labeled probes has been confirmed in many experimental studies. Sharma constructed an aptaswitch sensor complex using a combination of fluorophore and quenching labeled oligonucleotides and an aptamer that recognizes chloramphenicol [92,93]. Aptamer fluorescence detection uses the specific recognition of an aptamer and target antibiotics to regulate the energy transfer efficiency between a fluorescent donor and a recipient, to achieve quantitative detection of target antibiotics through changes in the fluorescence intensity or polarization [94,95].…”

Section: Fluorescent Aptamer Sensorsmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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Antibiotic abuse is becoming increasingly serious and the potential for harm to human health and the environment has aroused widespread social concern. Aminoglycoside antibiotics (AGs) are broad-spectrum antibiotics that have been widely used in clinical and animal medicine. Consequently, their residues are commonly found in animal-derived food items and the environment. A simple, rapid, and sensitive detection method for on-site screening and detection of AGs is urgently required. In recent years, with the development of molecular detection technology, nucleic acid aptamers have been successfully used as recognition molecules for the identification and detection of AGs in food and the environment. These aptamers have high affinities, selectivities, and specificities, are inexpensive, and can be produced with small batch-to-batch differences. This paper reviews the applications of aptamers for AG detection in colorimetric, fluorescent, chemiluminescent, surface plasmon resonance, and electrochemical sensors for the analysis in food and environmental samples. This study provides useful references for future research.

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Section: Fluorescent Aptamer Sensorsmentioning

confidence: 99%

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Luan

Wang

et al. 2020

Antibiotics

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“…(a) Synthesis route for B-1 and its condensation reactions with different non-boron motifs; (b) recognition patterns of the saturated vapors of the tested VSOMs at 293 K obtained by collecting fluorescence intensity variations of the sensors (S1 to S8). Note: the Arabic numerals in the figure stand for different chemicals: specifically, methane (1), ethane(2), propane (3), n-butane (4), n-pentane (5), n-hexane (6), n-heptane(7), n-octane(8), nnonane (9), n-decane(10), methanol (11), toluene(12), diethyl ether (13), benzene(14), acetone(15), ethanol(16), THF(17), DCM(18), TCM(19) and water(20). (c) Home-made sensing platform developed for VSOMs vapor detection[63] (color online).…”

mentioning

confidence: 99%

Recent advances in film-based fluorescence sensing

Liu¹,

Li²,

Liu³

et al. 2019

Sci. Sin.-Chim

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“…This result indicated that the dual-signal change of the ratio sensor can effectively improve the sensitivity of the uorescence aptasensor, and the homogeneous detection method improved the stability of the sensor. ELISA 0.24-2.21 0.13 [20] Liquid Chromatography 0.05-2 0.108 [21] Electrochemistry 0.05-20 0.016 [22] Fluorescence 0.1-0.8 0.07 [23] Fluorescence 0.05-100 0.05 [24] Fluorescence 0.015-15 0.003 [25] Fluorescence 0.05-100 0.0126 This work…”

Section: Detection Of Afb1mentioning

confidence: 94%

A Ratiometric Fluorescent Aptamer Homogeneous Biosensor Based on Hairpin Structure Aptamer for AFB1 Detection

Feng

Zhao

Wei

et al. 2022

Preprint

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On the basis of aptamer (Apt) with hairpin structure and fluorescence resonance energy transfer (FRET), a ratio fluorescent aptamer homogeneous sensor was prepared for the determination of Aflatoxin B1 (AFB1). Initially, the Apt labeled simultaneously with Cy5, BHQ2, and cDNA labeled with Cy3 were formed a double-stranded DNA through complementary base pairing. The fluorescent aptamer sensor demonstrates a weak fluorescence emission of Cy3 and a high fluorescence emission of Cy5 due to the quenching effect of BHQ2. The double-stranded DNA structure will be disintegrated in the presence of AFB1, resulting the removal of Cy3 and the close of Cy5 with BHQ2. The fluorescence signal of Cy3 and Cy5 were restored and quenched respectively. Thus, the ratio change of FCy3 to FCy5 was used to realized the detection of AFB1 with wider detection range and lower limit of detection (LOD). The response of the optimized protocol for AFB1 detection was wider linear range from 0.05 ng/mL to 100 ng/mL and the LOD was 12.6 pg/mL. The sensor designed in this strategy has the advantages of simple preparation and fast signal response. It has been used for the detection of AFB1 in labeled corn and wine, indicating it had good application potential in practical samples.

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Highly sensitive aflatoxin B1 sensor based on DNA-guided assembly of fluorescent probe and TdT-assisted DNA polymerization

Cited by 25 publications

References 24 publications

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Recent advances in film-based fluorescence sensing

A Ratiometric Fluorescent Aptamer Homogeneous Biosensor Based on Hairpin Structure Aptamer for AFB1 Detection

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