Highly sensitive amperometric measurement of alkaline phosphatase activity with glucose oxidase amplification

Ciana, Leopoldo Della; Bernacca, Giuliana; Bordin, F.; Fenu, S.; Garetto, F.

doi:10.1016/0022-0728(94)03694-x

Cited by 35 publications

(12 citation statements)

References 11 publications

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“…[2] Many of the devices reported rely on the use of carbon materials such as glassy carbon, [3] and carbon pastes. [4] Screen printing of the carbon ink for the fabrication of electrodes has realized commercial success in the glucose sensing field. [5] Developed for the printing industry, this thick-film technology has been adapted for the electronics industries and biosensor research.…”

Section: Introductionmentioning

confidence: 99%

Electrochemical Characterization of Commercial and Home-Made Screen-Printed Carbon Electrodes

2003

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Screen-printing technology is widely used for the mass-production of disposable electrochemical sensors. The practical utility of carbon screen-printed electrodes has been exploited, despite the fact that little is known about the nature of the electrode reactions. (Wang, J.; Pedrero, M.; Sakslumd, H.; Hammerich, O.; Pingarron, J. Electrochemical activation of screenprinted carbon strips. The Analyst 1996, 121 (3), [345][346][347][348][349][350]. Given the complexity of carbon electrodes in general, and differences in the composition of commercial carbon inks, the question arises as to how such differences and complexity affect their electrochemical reactivity. The aim of this

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Section: Introductionmentioning

confidence: 99%

Electrochemical Characterization of Commercial and Home-Made Screen-Printed Carbon Electrodes

2003

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“…It is probable to further improve the sensitivity of the DNA sensor through a bienzyme substrate-recycling. The highly sensitive amperometric measurements of AP have been reported including conjugating with glucose oxidase [35], horseradish peroxidase [36] or tyrosinase and quinoprotein glucose dehydrogenase [32]. In addition, a photolithographic gold ®lm electrode is suitable for standardization and miniaturization of preparing the DNA sensors.…”

Section: Discussionmentioning

confidence: 99%

Microfabricated Disposable DNA Sensors Based on Enzymatic Amplification Electrochemical Detection

Huang

Liu

et al. 2001

Electroanalysis

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A new electrochemical biosensing method was developed based on the immobilization of DNA probes on photolithographic gold film electrodes by self‐assembled monolayer technique. The complementary sequence was hybridized to the modified electrode and the hybridization reaction was carried out at 37 °C for 1 h. Avidin‐akaline phosphatase was coupled to the hybrid through avidin‐biotin complex and hydrolyzed to produce an electroactive α‐naphthol, which could be detected by different pulse voltammetry (DPV). Numerous factors including the concentration of the substrate and incubation time are optimized to maximize the sensitivity and speed in the assay time. The sensors were employed to detect PCR products relative to the hepatitis B plasmid. The method has a linear calibration range of 1.5 nmol/L–50 nmol/L and the detection limit is 30 fmol.

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“…The other methods for detection of aPase use fairly alkaline pHs, too, as can be seen from pH 9.6 for the chemiluminescent FIA method, pH 9.5 for the amperometric method, and pH 9.0 for the cyclic voltammetric method. 10,12,13 Only a few methods, including the one presented here, work at a moderate pH of 8. 5 The Tb 3+ −L 1 assay requires an incubation time of 20 min.…”

Section: Procedures For Determination Of Apasementioning

confidence: 99%

“…A one‐step method was described for the fluorometric determination of the activity of the enzyme catalase, based on the finding that H 2 O 2 bound to the europium (III)–tetracycline complex is consumed by catalase 7 . Several other methods for determination of aPase have been described, including spectrophotometry, 8 fluorometry, 9 flow injection analysis with chemiluminescence detection, 10 amperometry, 11,12 and cyclic voltammetry 13 . To our knowledge, terbium complexes have not thus far been described for the determination of aPase.…”

Section: Introductionmentioning

confidence: 99%

Sensitive Terbium Probes for Luminescent Determination of both Alkaline Phosphatase and Codeine Phosphate

Duerkop

Aleksandrova

Scripinets

et al. 2008

Annals of the New York Academy of Sciences

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New terbium complexes of 2-oxo-4-hydroxy-quinoline-3-carboxylic acid derivatives are reported for determination of alkaline phosphatase (aPase) and codeine phosphate (CP). Complexation with terbium occurs by way of the oxygen atoms of the 4-hydroxyl group and the carbonyl group of the 3-carboxy function, respectively. The complexes are highly luminescent and do not require luminescence enhancers. The excitation maxima of the terbium chelates are at 320 nm. The assay is based on the quenching of the hypersensitive 545-nm luminescence of the terbium complexes with the ligands 4-hydroxy-1-methyl-2-oxo-1,2-dihydro-quinoline-3-carboxylic acid (5-ethyl-[1,3,4]thiadiazol-2-yl)-amide (L(1)) and 1-ethyl-4-hydroxy-2-oxo-1,2-dihydro-quinoline-3-carboxylic acid (4-trifluoromethyl-phenyl)-amide (L(2)) by phosphate ions. The assays require equimolar (1.0 microM) concentrations of Tb(3+) and of the ligand at pH 8.0 (Tris-HCl buffer). The luminescence quenching is proportional to the concentration of aPase and CP within the range of 0.1-70 mU mL(-1) and 0.3-20 microg mL(-1), respectively. The detection limits were 0.05 mU mL(-1)=40.0 pmol L(-1) of aPase and 0.120 microg mL(-1) of CP.

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Highly sensitive amperometric measurement of alkaline phosphatase activity with glucose oxidase amplification

Cited by 35 publications

References 11 publications

Electrochemical Characterization of Commercial and Home-Made Screen-Printed Carbon Electrodes

Electrochemical Characterization of Commercial and Home-Made Screen-Printed Carbon Electrodes

Microfabricated Disposable DNA Sensors Based on Enzymatic Amplification Electrochemical Detection

Sensitive Terbium Probes for Luminescent Determination of both Alkaline Phosphatase and Codeine Phosphate

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