Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Lv, Yanbing; Wu, Ruili; Feng, Kunrui; Li, Jinjie; Mao, Qing; Yuan, Hang; Shen, Huaibin; Chai, Xiangdong; Li, Lin Song

doi:10.1186/s12951-017-0267-4

Cited by 74 publications

(32 citation statements)

References 40 publications

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“…Consequently, the thick shell InP/GaP/ZnS core/shell QDs with high PL QY and high stability are identified as the optimized fluorescent label material in our study. In addition, the analytical performance of this method has been compared with other previously reported immunoanalysis studies for CRP detection . From Table 1 , compared with the Cd‐free QD system immunoassay, the thick shell InP/GaP/ZnS QD‐FLISA shows high sensitivity and broad detection range, which can be comparable to the Cd‐based QD immunoassay.…”

Section: Resultsmentioning

confidence: 97%

Preparation of Highly Stable and Photoluminescent Cadmium‐Free InP/GaP/ZnS Core/Shell Quantum Dots and Application to Quantitative Immunoassay

et al. 2020

Part & Part Syst Charact

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Indium phosphide (InP) quantum dots (QDs) are ideal substitutes for widely used cadmium‐based QDs and have great application prospects in biological fields due to their environmentally benign properties and human safety. However, the synthesis of InP core/shell QDs with biocompatibility, high quantum yield (QY), uniform particle size, and high stability is still a challenging subject. Herein, high quality (QY up to 72%) thick shell InP/GaP/ZnS core/shell QDs (12.8 ± 1.4 nm) are synthesized using multiple injections of shell precursor and extension of shell growth time, with GaP serving as the intermediate layer and 1‐octanethiol acting as the new S source. The thick shell InP/GaP/ZnS core/shell QDs still keep high QY and photostability after transfer into water. InP/GaP/ZnS core/shell QDs as fluorescence labels to establish QD‐based fluorescence‐linked immunosorbent assay (QD‐FLISA) for quantitative detection of C‐reactive protein (CRP), and a calibration curve is established between fluorescence intensity and CRP concentrations (range: 1–800 ng mL−1, correlation coefficient: R2 = 0.9992). The limit of detection is 2.9 ng mL−1, which increases twofold compared to previously reported cadmium‐free QD‐based immunoassays. Thus, InP/GaP/ZnS core/shell QDs as a great promise fluorescence labeling material, provide a new route for cadmium‐free sensitive and specific immunoassays in biomedical fields.

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Section: Resultsmentioning

confidence: 97%

Preparation of Highly Stable and Photoluminescent Cadmium‐Free InP/GaP/ZnS Core/Shell Quantum Dots and Application to Quantitative Immunoassay

et al. 2020

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“…Enhanced immunoturbidimetry assay was utilized as a reference method because it was widely adopted as a gold standard test for in vitro diagnostic (14). However, the semi -quantitative slide method is rapid and inexpensive method to measure serum CRP concentration.…”

Section: Discussionmentioning

confidence: 99%

“Comparison of a Rapid Semi-Quantitative Latex Agglutination Slide Method Against Quantitative Particle Enhanced Turbidimetric Immunoassay for Measurement of C- Reactive Protein”

Trivedi

Amer

Patel

et al. 2019

IJMBS

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Serum C -reactive protein (CRP) is an acute phase marker in humans that is useful for the diagnosis and monitoring of inflammatory disease. CRP measurement also helps in differential diagnosis, in the management of neonatal septicaemia and meningitis where standard microbiological investigations are very much timeconsuming. Reliable method (Automated particle enhanced turbidimetric immunoassay) are preferable for routine evaluation of human serum CRP levels. Using such expensive methods in health centres in rural areas is not possible. The aim of this to evaluate whether human CRP levels could be measured by rapid method (Latex agglutination slide method) and compared with reliable method (particle enhanced turbidimetric immunoassay).The study included 400 participants, who attended Shree Krishna Hospital, Karamsad, whose CRP levels were done by particle enhanced turbidimetric immunoassay method in Siemens Dimension Clinical Chemistry Analyzer, RxL and Xpand and compared by rapid method (Latex Agglutination slide method), that will be useful in health centres in rural areas where expensive instruments are not available. We used SPSS for data analysis and Endnote X7 for references management. In present study, out of 400 (217 male and 183 female) participants, we found 337 participants had positive CRP levels (≥0.3 mg/dl) and 63 participants had negative CRP levels (<0.3 mg/dl) by particle enhanced turbidimetric immunoassay method, whereas 303 had positive CRP levels (≥0.6mg/dl) and 97 had negative CRP levels (<0.6mg/dl) by Latex Agglutination slide method. The correlation was positive between two methods with (r = 0.764, P -value <0.0001). The sensitivity, specificity, positive predictive value (PPV) and negative predictive value (NPV) were 89.9%, 100%, 100%, and 64.94%, respectively, for the Latex Agglutination slide method, and 100%, 64.9%, 89.91% and 100%, respectively, for the particle enhanced turbidimetric immunoassay method. Serum C-reactive protein (CRP) levels can be reliably measured using the particle enhanced turbidimetric immunoassay method, but it is expensive, so we can use Latex agglutination slide method in rural areas for primary diagnosis only.

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“…Immunoturbidimetry is fast and sensitive, but requires expensive instrumentation; ELISAs are economical by comparison, but often take longer than an hour to develop [54]. The total time required for the FLISAs was 50 mins, shorter than a typical ELISA assay, showing potential for development into a rapid and costeffective diagnostic tool [4].…”

Section: Quantum Dot Fundamentalsmentioning

confidence: 99%

Sensing with photoluminescent semiconductor quantum dots

Chern

Kays

Bhuckory

et al. 2019

Methods Appl. Fluoresc.

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Fluorescent sensors benefit from high signal-to-noise and multiple measurement modalities, allowing for a multitude of applications and flexibility of design. Semiconductor nanocrystal quantum dots (QDs) are excellent fluorophores for sensors because of their extraordinary optical properties. They have high thermal and photochemical stability compared to organic dyes or fluorescent proteins and are extremely bright due to their large molar cross-sections. In contrast to organic dyes, QD emission profiles are symmetric, with relatively low full width half maxima. In addition, the size tunability of their emission color, which is a result of quantum confinement, make QDs exceptional emitters with high color purity from the ultraviolet to near infrared wavelength range. The role of QDs in sensors ranges from simple fluorescent tags, as used in immunoassays, to intrinsic sensors that utilize the inherent photophysical response of QDs to fluctuations in temperature, electric field or ion concentration. In more complex configurations, QDs and biomolecular recognition moieties like antibodies are combined with a third component to transduce the optical signal via energy transfer. QDs can act as donors, acceptors, or both in energy transfer-based sensors using Förster resonance energy transfer (FRET), nanometal surface energy transfer (NSET), or charge or electron transfer. The changes in both spectral response and photoluminescent lifetimes have been successfully harnessed to produce more sensitive sensors and multiplexed devices. While technical challenges related to biofunctionalization and the high cost of laboratory-grade fluorimeters have thus far prevented broad implementation of QD-based sensing in clinical or commercial settings, improvements in bioconjugation methods and detection schemes, including using simple consumer devices like cell phone cameras, are lowering the barrier to broad use of more sensitive QD-based devices.

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Highly sensitive and accurate detection of C-reactive protein by CdSe/ZnS quantum dot-based fluorescence-linked immunosorbent assay

Cited by 74 publications

References 40 publications

Preparation of Highly Stable and Photoluminescent Cadmium‐Free InP/GaP/ZnS Core/Shell Quantum Dots and Application to Quantitative Immunoassay

Preparation of Highly Stable and Photoluminescent Cadmium‐Free InP/GaP/ZnS Core/Shell Quantum Dots and Application to Quantitative Immunoassay

“Comparison of a Rapid Semi-Quantitative Latex Agglutination Slide Method Against Quantitative Particle Enhanced Turbidimetric Immunoassay for Measurement of C- Reactive Protein”

Sensing with photoluminescent semiconductor quantum dots

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