Highly Sensitive and High-Throughput Analysis of Plant Hormones Using MS-Probe Modification and Liquid Chromatography–Tandem Mass Spectrometry: An Application for Hormone Profiling in Oryza sativa

Kojima, Mikiko; Kamada-Nobusada, Tomoe; Komatsu, Hirokazu; Takei, Kentaro; Kuroha, Takeshi; Mizutani, Masaharu; Ashikari, Motoyuki; Ueguchi-Tanaka, Miyako; Matsuoka, Makoto; Suzuki, Kôji; Sakakibara, Hitoshi

doi:10.1093/pcp/pcp057

Cited by 429 publications

(381 citation statements)

References 51 publications

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“…Extraction and determination of GA of S. moellendorffii microsporangia and young stems were performed using a liquid chromatography-tandem mass chromatography system (AQUITY UPLC System/Quattro Premier XE, Waters) as described 41 . Data were processed by MassLynx software with QuanLynx (version 4.0, Waters).…”

Section: Methodsmentioning

confidence: 99%

The Gibberellin perception system evolved to regulate a pre-existing GAMYB-mediated system during land plant evolution

et al. 2011

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Gibberellin (GA) controls pollen development in flowering plants via the GAMYB transcription factor. Here we show that GAMYB is conserved in Selaginella moellendorffii (lycophyte) and Physcomitrella patens (moss), although the former contains the GA signalling pathway, the latter does not. In the lycophyte, GA treatment promotes the outer wall development on microspores, whereas treatment with GA biosynthesis inhibitors disturbs its development. Contrary, in the moss, GAMYB homologue knockouts also produce abnormal spores that resemble Selaginella microspores treated with GA biosynthesis inhibitors and pollen grains of rice gamyb mutant. Moreover, the knockouts fail to develop male organs, instead ectopically forming female organs. Thus, before the establishment of the GA signalling pathway, basal land plants, including mosses, contained a GAMYB-based system for spore and sexual organ development. Subsequently, during the evolution from mosses to basal vascular plants including lycophytes, GA signalling might have merged to regulate this pre-existing GAMYB-based system.Peer reviewe

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Section: Methodsmentioning

confidence: 99%

The Gibberellin perception system evolved to regulate a pre-existing GAMYB-mediated system during land plant evolution

et al. 2011

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“…Extraction and determination of ABA were performed as described previously (Kojima et al, 2009). ABA was measured by ultra-performance liquid chromatography coupled with a tandem quadrupole mass spectrometer equipped with an electrospray interface (Aquity UPLCTM System/Quattro Premier XE; Waters) with an ODS column (Aquity UPLC BEH C 18 [1.7 mm, 2.1 3 100 mm]; Waters).…”

Section: Quantitative Analysis Of Abamentioning

confidence: 99%

Enhanced Stomatal Conductance by a Spontaneous Arabidopsis Tetraploid, Me-0, Results from Increased Stomatal Size and Greater Stomatal Aperture

et al. 2016

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The rate of gas exchange in plants is regulated mainly by stomatal size and density. Generally, higher densities of smaller stomata are advantageous for gas exchange; however, it is unclear what the effect of an extraordinary change in stomatal size might have on a plant's gas-exchange capacity. We investigated the stomatal responses to CO 2 concentration changes among 374 Arabidopsis (Arabidopsis thaliana) ecotypes and discovered that Mechtshausen (Me-0), a natural tetraploid ecotype, has significantly larger stomata and can achieve a high stomatal conductance. We surmised that the cause of the increased stomatal conductance is tetraploidization; however, the stomatal conductance of another tetraploid accession, tetraploid Columbia (Col), was not as high as that in Me-0. One difference between these two accessions was the size of their stomatal apertures. Analyses of abscisic acid sensitivity, ion balance, and gene expression profiles suggested that physiological or genetic factors restrict the stomatal opening in tetraploid Col but not in Me-0. Our results show that Me-0 overcomes the handicap of stomatal opening that is typical for tetraploids and achieves higher stomatal conductance compared with the closely related tetraploid Col on account of larger stomatal apertures. This study provides evidence for whether larger stomatal size in tetraploids of higher plants can improve stomatal conductance.

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“…pollen, and the endogenous GA levels were quantified with ultra-performance liquid chromatography (UPLC)-tandem mass spectrometry (Kojima et al, 2009). The results indicated that the dominant bioactive GAs in pollen were GA 4 and GA 7 and that the levels significantly increased during pollen maturation under normal conditions (Fig.…”

Section: Lt Reduces the Endogenous Levels Of Bioactive Gas In Developmentioning

confidence: 99%

“…GAs were extracted from 100 mg of cv Sasanishiki anthers dissected at meiotic, uninucleate, binucleate, and trinucleate pollen stages with or without LT treatment, and their identities were determined using UPLC-tandem mass spectrometry (AQUITY UPLC System/XEVO-TQS; Waters) with an octadecylsilyl column (AQUITY UPLC BEH C18, 1.7 mm, 2.1 3 100 mm; Waters) as described previously (Kojima et al, 2009). Each series of experiments was performed in biological triplicate.…”

Section: Quantification Of Gasmentioning

confidence: 99%

Reduction of Gibberellin by Low Temperature Disrupts Pollen Development in Rice

et al. 2014

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Microsporogenesis in rice (Oryza sativa) plants is susceptible to moderate low temperature (LT; approximately 19°C) that disrupts pollen development and causes severe reductions in grain yields. Although considerable research has been invested in the study of cool-temperature injury, a full understanding of the molecular mechanism has not been achieved. Here, we show that endogenous levels of the bioactive gibberellins (GAs) GA 4 and GA 7 , and expression levels of the GA biosynthesis genes GA20ox3 and GA3ox1, decrease in the developing anthers by exposure to LT. By contrast, the levels of precursor GA 12 were higher in response to LT. In addition, the expression of the dehydration-responsive element-binding protein DREB2B and SLENDER RICE1 (SLR1)/DELLA was up-regulated in response to LT. Mutants involved in GA biosynthetic and response pathways were hypersensitive to LT stress, including the semidwarf mutants sd1 and d35, the gain-of-function mutant slr1-d, and gibberellin insensitive dwarf1. The reduction in the number of sporogenous cells and the abnormal enlargement of tapetal cells occurred most severely in the GA-insensitive mutant. Application of exogenous GA significantly reversed the male sterility caused by LT, and simultaneous application of exogenous GA with sucrose substantially improved the extent of normal pollen development. Modern rice varieties carrying the sd1 mutation are widely cultivated, and the sd1 mutation is considered one of the greatest achievements of the Green Revolution. The protective strategy achieved by our work may help sustain steady yields of rice under global climate change.

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Highly Sensitive and High-Throughput Analysis of Plant Hormones Using MS-Probe Modification and Liquid Chromatography–Tandem Mass Spectrometry: An Application for Hormone Profiling in Oryza sativa

Cited by 429 publications

References 51 publications

The Gibberellin perception system evolved to regulate a pre-existing GAMYB-mediated system during land plant evolution

The Gibberellin perception system evolved to regulate a pre-existing GAMYB-mediated system during land plant evolution

Enhanced Stomatal Conductance by a Spontaneous Arabidopsis Tetraploid, Me-0, Results from Increased Stomatal Size and Greater Stomatal Aperture

Reduction of Gibberellin by Low Temperature Disrupts Pollen Development in Rice

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