Highly sensitive and label-free electrochemical detection of C-reactive protein on a peptide receptor−gold nanoparticle−black phosphorous nanocomposite modified electrode

Yang, Hyo Jeong; Kim, Min Woo; Raju, Chikkili Venkateswara; Cho, Chae Hwan; Park, Tae Jung; Park, Jong Pil

doi:10.1016/j.bios.2023.115382

Cited by 24 publications

(12 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This binding affinity allows the surface to effectively adsorb these molecules, providing good performance and sensitivity for the sensor. 94 A recent example is the construction by Ding et al of a ratiometric antifouling sensor based on a Y-type peptide and loaded Au@ZIF-67 with MXene of methylene blue. AuNPs can be used as binding sites for thiol-modified biomolecules, which successfully resulted in a novel nanocomposite with high productivity, large specific surface area, and abundant biomolecule binding sites (Fig.…”

Section: Nanomaterials In Biosensing Technologiesmentioning

confidence: 99%

“…7c). 94 Electrochemical biosensors constructed based on CNTs are the most widely used type, with advantages in size and sensitivity as well as response. CNTs have been widely used to facilitate electron transfer and construct enzyme-based electrochemical biosensors due to their small size, electrochemical properties, and high specific surface area.…”

Section: Nanomaterials In Biosensing Technologiesmentioning

confidence: 99%

See 1 more Smart Citation

A review of nanomaterials for biosensing applications

Li,

Wang,

Zhong

et al. 2024

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

Section: Nanomaterials In Biosensing Technologiesmentioning

confidence: 99%

Section: Nanomaterials In Biosensing Technologiesmentioning

confidence: 99%

A review of nanomaterials for biosensing applications

Li,

Wang,

Zhong

et al. 2024

J. Mater. Chem. B

View full text Add to dashboard Cite

show abstract

“…Antibodies are currently the most frequently used CRP receptors. 24,29,30 In the literature, there are also examples of CRP determination using bioreceptors such as aptamers, 31−35 bacteriophages, 36 affimers, 37 peptides, 38,39 or nanobodies. 40 CRP assays are often performed using biological methods such as ELISA, 41 test-based lateral flow immunoassays, and other immunoassays.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Antibodies are currently the most frequently used CRP receptors. ,, In the literature, there are also examples of CRP determination using bioreceptors such as aptamers, – bacteriophages, affimers, peptides, , or nanobodies …”

Section: Introductionmentioning

confidence: 99%

“…Only a couple of such peptides have been identified previously, one longer peptide (15 mer) and one very short peptide (3–5 mer) showing a very limited operation range . The 12 mer peptides binding to CRP were identified using the phage display . However, these peptides were combined with the two extra gold binding sequences (28 mer), so the final size of the peptide recognition element was 40 mer.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Investigation of Peptides for Molecular Recognition of C-Reactive Protein–Theoretical and Experimental Studies

Szot-Karpińska,

Kudła,

Orzeł

et al. 2023

Anal. Chem.

View full text Add to dashboard Cite

We investigate the interactions between C-reactive protein (CRP) and new CRP-binding peptide materials using experimental (biological and physicochemical) methods with the support of theoretical simulations (computational modeling analysis). Three specific CRP-binding peptides (P2, P3, and P9) derived from an M13 bacteriophage have been identified using phage-display technology. The binding efficiency of the peptides exposed on phages toward the CRP protein was demonstrated via biological methods. Fibers of the selected phages/peptides interact differently due to different compositions of amino acid sequences on the exposed peptides, which was confirmed by transmission electron microscopy. Numerical and experimental studies consistently showed that the P3 peptide is the best CRP binder. A combination of theoretical and experimental methods demonstrates that identifying the best binder can be performed simply, cheaply, and fast. Such an approach has not been reported previously for peptide screening and demonstrates a new trend in science where calculations can replace or support laborious experimental techniques. Finally, the best CRP binderthe P3 peptidewas used for CRP recognition on silicate-modified indium tin oxide-coated glass electrodes. The obtained electrodes exhibit a wide range of operation (1.0–100 μg mL–1) with a detection limit (LOD = 3σ/S) of 0.34 μg mL–1. Moreover, the dissociation constant K d of 4.2 ± 0.144 μg mL–1 (35 ± 1.2 nM) was evaluated from the change in the current. The selectivity of the obtained electrode was demonstrated in the presence of three interfering proteins. These results prove that the presented P3 peptide is a potential candidate as a receptor for CRP, which can replace specific antibodies.

show abstract