Highly sensitive and selective cholesterol biosensor based on direct electron transfer of hemoglobin

Cheng, Zhiqing; Wan, Li; Jiang, Li; Wang, Qin; Jiao, Kui

doi:10.1016/j.ab.2008.08.022

Cited by 61 publications

(30 citation statements)

References 49 publications

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“…All other chemicals were of analytical grade or higher. A stock solution (5.00 × 10 À3 mol/l) of cholesterol was prepared, as described previously (0.0484 g cholesterol in 25 ml water with 1 ml isopropanol and 0.5 ml Triton X-100) (8). Working solutions of cholesterol were freshly prepared by diluting the stock solution with PBS (pH 7.0) containing 1% (v/v) Triton X-100.…”

Section: Experimental Materials and Reagentsmentioning

confidence: 99%

“…Various methods have been reported for the determination of cholesterol, including spectrophotometry (3), fluorescent spectrometry (4,5), chemiluminescence (6), high-performance liquid chromatography (7) and electrochemical methods (8)(9)(10)(11). Some of these have certain disadvantages, such as scarce reagents (3,5), expensive equipment (7), poor selectivity or sensitivity (8,9) or excessive operation times (10,11).…”

Section: Introductionmentioning

confidence: 99%

“…Some of these have certain disadvantages, such as scarce reagents (3,5), expensive equipment (7), poor selectivity or sensitivity (8,9) or excessive operation times (10,11). A few of the analytical methods reported in recent years have led to advancements in the determination of cholesterol in specific samples, however, special pretreatment or techniques are usually needed (12).…”

Section: Introductionmentioning

confidence: 99%

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Highly sensitive and selective electrochemiluminescence determination of cholesterol utilizing a functional electrode with a core–shell nanostructure

Cheng

2015

Luminescence

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A highly sensitive and selective method for the determination of cholesterol is required to evaluate trace amounts of cholesterol in test samples. In this work, selected gold nanoparticles (AuNPs) and 5-amino-2-mercapto-1,3,4-thiadiazole (AMT) were used and a thin film of three-dimensional gold-AMT core-shell nanoparticles (p-AMT-AuNPs) was prepared using an electrochemical method. Cholesterol oxidase was then bonded to the film surface to give a functional electrode. Based on catalysis by the electrode functionalized for cholesterol and a luminol-H2 O2 electrochemiluminescence (ECL) system, a highly sensitive and selective ECL method was developed for the determination of cholesterol. Under optimized conditions, ECL intensity showed a good linear relationship with cholesterol over the concentration range 0.05-11.0 µg/ml, with a correlation coefficient of 0.999 and a limit of detection of 0.02 µg/ml. The proposed method was used to determine cholesterol in dairy products with a relative standard deviation of < 1.8% and recovery rates of 98.1-104%.

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Section: Experimental Materials and Reagentsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Highly sensitive and selective electrochemiluminescence determination of cholesterol utilizing a functional electrode with a core–shell nanostructure

Cheng

2015

Luminescence

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“…In addition the immobilization of enzymes allows a low consumption of enzyme. The main immobilizing approaches adopted for cholesterol biosensor include electropolymerization entrapment technique [3,6] self assembled monolayers [7,8] lipid bilayer memberane [9] anodic porous alumina [10] graphiteTeflon composite [11] Prussian blue/poly pyrrole composite 12] direct adsorption [13] chitosan film [14] and sol-gel composite film [15]. A short life time, the waste of expensive biocatalyst (ChOx), leaching of enzyme from electrode surfaces, low catalytic activity, low reproducibility of the biosensor fabrication method and needing electron transfer mediator are main disadvantages of the cholesterol biosensors.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of a Sensitive Cholesterol Biosensor Based on Cobalt‐oxide Nanostructures Electrodeposited onto Glassy Carbon Electrode

2009

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Electrodeposited cobalt oxide (CoOx) nanomaterials are not only used for immobilization of cholesterol oxidase (ChOx) but also as electron transfer mediator for oxidation of H 2 O 2 generated in the enzymatic reaction. Voltammetry and flow injection analysis (FIA) were used for determination of cholesterol. FIA determination of cholesterol with biosensors yielded a calibration curve with the following characteristics: linear range up to 50 mM, sensitivity of 43.5 nA mM À1 cm À2 and detection limit of 4.2 mM. The apparent Michaelis-Menten constant and the response time of the biosensor are 0.49 mM and 15 s, respectively. This biosensor also exhibits good stability, reproducibility and long life time.

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“…[25][26][27] Recently, the direct electrochemistry of Hb has been described in some papers and used to develop H2O2 biosensors. [28][29][30][31][32][33][34][35][36][37][38][39] In addition, a bienzyme channeling glucose sensor based on the co-entrapment of glucose oxidase (GOD) and horseradish peroxidase (HRP) in the mesopores of SBA-15 has been developed; 40 the detection of glucose has been accomplished via an electrocatalytic reaction with the HRP to the reduction of H2O2 produced from the enzymatic reaction of a GOD-glucose-dissolved oxygen system. Herein, the direct electron transfer (DET) between Hb and electrode resembles a redox mediator.…”

Section: Introductionmentioning

confidence: 99%

Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

et al. 2009

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A uric acid biosensor based on the direct electron transfer of a hemoglobin-encapsulated chitosan-modified glassy carbon electrode was developed for a highly sensitive and selective analysis in urine samples. The modified electrode was prepared by the encapsulation of hemoglobin and uricase in a chitosan matrix. The hydrogen peroxide produced from the catalytic oxidation of uric acid by uricase was reduced electrocatalytically by immobilized hemoglobin and used to obtain a sensitive amperometric response to uric acid. The linear response of the uric acid concentrations ranged from 2.00 to 30.0 µM with a correlation of 0.9982, the detection limit of uric acid was estimated to be 0.85 µM at a signal/noise ratio of 3. The uric acid biosensor can efficiently exclude the interference of commonly coexisted ascorbic acid, dopamine, epinephrine, etc. The relative standard deviation was under 2.56% (n = 5) for the determination of real samples. This biosensor is satisfactory for the determination of human urine samples compared with the HPLC-UV method.

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Highly sensitive and selective cholesterol biosensor based on direct electron transfer of hemoglobin

Cited by 61 publications

References 49 publications

Highly sensitive and selective electrochemiluminescence determination of cholesterol utilizing a functional electrode with a core–shell nanostructure

Highly sensitive and selective electrochemiluminescence determination of cholesterol utilizing a functional electrode with a core–shell nanostructure

Fabrication of a Sensitive Cholesterol Biosensor Based on Cobalt‐oxide Nanostructures Electrodeposited onto Glassy Carbon Electrode

Highly Sensitive and Selective Uric Acid Biosensor Based on Direct Electron Transfer of Hemoglobin-encapsulated Chitosan-modified Glassy Carbon Electrode

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