Highly sensitive and selective colorimetric determination ofStaphylococcus aureus viachicken anti-protein A IgY antibody

Abstract

Utilization of chicken anti-protein A IgY as an antibody pair for sensitive and selective detection of S. aureus.

Advances in Chicken IgY-Based Immunoassays for the Detection of Chemical and Biological Hazards in Food Samples

“…Several papers have reported the detection of S. aureus by producing IgY against SPA. As shown in Figure A, Zhang et al developed a colorimetric immunoassay for detecting S. aureus based on the specific recognition of SPA and IgY . Two IgY antibodies that act as capture and detection antibodies were conjugated with magnetic particles and horseradish peroxidase (HRP), respectively, to perform a sandwich immunoassay.…”

“…(B) IgY was used as the capture antibody, while the Fc region of IgG was used as the detection antibody. Part A is reprinted with permission from Zhang et al (ref ). Part B is reprinted with permission from Zhang et al (ref ).…”

A novel colorimetric sensing platform for the detection ofS. aureuswith high sensitivity and specificity

“…38 Recently, several methods relying on the recognition of SPA have been put forwarded for the detection of S. aureus. 17,32,39,40 For example, a chemiluminescent method for S. aureus detection was established by utilizing SPA-modied magnetic beads as competitive bacterial analogues and HRP labeled IgG as tracer, which could be completed in 50 min with a low detection limit of 6 CFU mL À1 , 17 while the presence of protein G producing Streptococcus would also result in positive detection signals. Dual-recognition strategies for S. aureus detection were based on vancomycin-affinity, utilizing IgG as the second recognition agent to bind with SPA.…”

“…Chicken anti-protein A IgY was previously utilized as an antibody pair for the quantitative detection of S. aureus with high sensitivity and selectivity. 40 Despite its desired selectivity, the consumption of chicken anti-protein A IgY that served as capture antibody was relatively high, resulting in higher cost of the test. To lower the test cost, dog IgG was introduced as a capture agent to detect S. aureus in the present study.…”