Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors

Misawa, Nobuo; Mitsuno, Hidefumi; Kanzaki, Ryohei; Takeuchi, Shoji

doi:10.1073/pnas.1004334107

Cited by 113 publications

(64 citation statements)

References 33 publications

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“…Comprehensive reviews exist for bone, breast, cardiac, corneal, liver and tumor tissue models [51-53] as well as neural networks [54,55]; we expand on this literature by focusing more on liver and tumor tissue models. Other on-chip models that could be used for future studies on drug discovery and toxicity include those for sensory organs such as the nose [41,56,57] and biological processes such as sperm motility [42,43,58] and wound healing [59,60]. …”

Section: Tissue Models On a Chipmentioning

confidence: 99%

Biomimetic tissues on a chip for drug discovery

Ghaemmaghami

Hancock

Harrington

et al. 2012

Drug Discovery Today

333

247

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Teaser Recent advances in tissue engineering have enabled the development of microscale biomimetic ‘organ on a chip’ tissue models which have the potential to make an important impact on the various stages of drug discovery and toxicity testing. Developing biologically relevant models of human tissues and organs is an important enabling step for disease modeling and drug discovery. Recent advances in tissue engineering, biomaterials and microfluidics have led to the development of microscale functional units of such models also referred to as ‘organs on a chip’. In this review, we provide an overview of key enabling technologies and highlight the wealth of recent work regarding on-chip tissue models. In addition, we discuss the current challenges and future directions of organ-on-chip development.

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Section: Tissue Models On a Chipmentioning

confidence: 99%

Biomimetic tissues on a chip for drug discovery

Ghaemmaghami

Hancock

Harrington

et al. 2012

Drug Discovery Today

333

247

View full text Add to dashboard Cite

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“…With continued advances in analytical chemistry techniques (Misiwa et al, 2010), more challenges can be expected in the area of discovering and evaluating the safety of very low levels of contaminants in food. Because the TTC approach offers the potential to greatly simplify the assessment and prioritization of chemical risks, it is important that the rigor, and thus the legitimacy, of the approach be maintained.…”

Section: Discussionmentioning

confidence: 99%

Determining the Applicability of Threshold of Toxicological Concern Approaches to Substances Found in Foods

Canady

Lane

Paoli³

et al. 2013

Critical Reviews in Food Science and Nutrition

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Threshold of Toxicological Concern (TTC) decision-support methods present a pragmatic approach to using data from well-characterized chemicals and protective estimates of exposure in a stepwise fashion to inform decisions regarding low-level exposures to chemicals for which few data exist. It is based on structural and functional categorizations of chemicals derived from decades of animal testing with a wide variety of chemicals. Expertise is required to use the TTC methods, and there are situations in which its use is clearly inappropriate or not currently supported. To facilitate proper use of the TTC, this paper describes issues to be considered by risk managers when faced with the situation of an unexpected substance in food. Case studies are provided to illustrate the implementation of these considerations, demonstrating the steps taken in deciding whether it would be appropriate to apply the TTC approach in each case. By appropriately applying the methods, employing the appropriate scientific expertise, and combining use with the conservative assumptions embedded within the derivation of the thresholds, the TTC can realize its potential to protect public health and to contribute to efficient use of resources in food safety risk management.

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“…This epithelial pattern likely results from both the intrinsic OSN sensitivity and the sorptive patterns imposed by the interaction of nasal aerodynamics with physiochemical properties of odorants, although the precise contributions of each are ill-defined. Misawa et al (91) reported a highly sensitive and selective chemical sensor using living cells (Xenopus laevis oocytes) within a portable fluidic device. This odorant sensor's sensitivity was a few parts per billion in solution and the sensor could simultaneously distinguish different types of chemicals that have only slight differences in double-bond isomerism or functional groups such as -OH, -CHO, and -C(=O)-.…”

Section: Quantitative Problems Of Testing Olfactory Samplesmentioning

confidence: 99%

Recent Advances in Olfactory Receptor (OR) Biosensors and Cell Signaling Cascade Amplification Systems

2018

Sensors and Materials

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There are a variety of methods to detect odorant molecules, including gas chromatography (GC) and its related coupled techniques, electronic noses, and olfactory receptor (OR)-based biosensors. Nevertheless, studies in which cell and cell signaling amplifier systems were directly utilizied to detect odors are very few. In this review, we aim to provide some references and suggestions for researchers in related fields by summarizing OR sensors and olfactory cell signaling cascade amplifier systems. In this paper, we summarize the detection methods for odorants, introduce the research and progress on OR sensors, and present a proposal of utilizing cell or tissue signaling cascade amplifier systems to amplify electrochemical signals and the use of the G protein signaling cascade amplifier system to prepare electrochemical biosensors. In recent years, the detection technology of electrochemical sensors has matured. Meanwhile, olfactory sensation within organisms basically transfer nerve signals or metabolic endocrine signals in the form of electrochemical signals. Therefore, ORs combined with cell signaling cascade amplifier systems, as well as electrochemical sensors of signal cascade amplifier systems, have incredible prospects for development and application. The proposal of OR electrochemical biosensors and their cell signaling cascade amplifier systems, provides a new idea and a quantitative method for the detection and assessment of odorants and their physiologic processes in the nervous system.

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Highly sensitive and selective odorant sensor using living cells expressing insect olfactory receptors

Cited by 113 publications

References 33 publications

Biomimetic tissues on a chip for drug discovery

Biomimetic tissues on a chip for drug discovery

Determining the Applicability of Threshold of Toxicological Concern Approaches to Substances Found in Foods

Recent Advances in Olfactory Receptor (OR) Biosensors and Cell Signaling Cascade Amplification Systems

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