Highly Sensitive and Selective Sensing Probe for Determination of Anti-Covid-19 Remdesvir: Application to Pharmaceutical Dosage Form and Biological Fluids

Abstract

We report the first investigational electrochemical study for Remdesvir (REM), a promising antiviral agent used recently for the treatment of COVID-19. An anionic surfactant, silica nanoparticles, and multiwall carbon nanotubes carbon paste (SDS/SiO2/MWCNT/CPE) sensor was designed to introduce our approach. The results revealed irreversible diffusion oxidative reaction of REM with two well-defined peaks (E1/V=1.19, E2/V=1.35) in 0.1 M phosphate buffer of pH 6 using differential pulse (DP) voltammetry. A linear relationship between the peak current and the drug concentration was established over the concentration range of 1.66 x 10-7 - 3.52 x 10-6 M (100-200 ng/mL) with a limit of detection (LOD) of 4.80 x 10-8 M and limit of quantitation (LOQ) of 8.0 x 10-8 M and mean % recovery ± % RSD of 99.05 ± 1.94. The proposed method succeeded in determination of the drug in its pharmaceutical dosage form, in human plasma, and in human urine samples. Finally, the method was validated according to ICH guidelines and FDA guidance for the determination of the drug in biological fluids. The developed data was found to be in good agreement with a validated reported method.

Solid-state ion-selective electrodes for the first potentiometric determination of the anti-COVID 19 drug Remdesivir in human plasma; A comparative study

“…Several chromatographic [3] , [4] , [5] , [6] , spectrophotometric [4] , [7] and voltammetric methods [8] have been reported for (RDS) analysis. However, to the best of our knowledge, no potentiometric methods have been developed yet for (RDS).…”