Highly sensitive aptamer based on electrochemiluminescence biosensor for label-free detection of bisphenol A

Abstract

Bisphenol A (BPA), a typical endocrine disruptor, is widely used as a key monomer in the packaging industry. Residual monomer can transfer from the package material to the food and thereby pose a risk to the health of the consumer, so determination of BPA migration is highly important for food safety control. In this study, a simple but sensitive electrochemiluminescence (ECL) biosensor, which combines the characteristics of high selectivity of an aptamer and high sensitivity of ECL, has been developed to detect BPA from package materials. The aptamer was immobilized on a gold electrode surface through Au-S interaction. The aptamer was then hybridized with complementary DNA (CDNA) to form double-stranded DNA (dsDNA). Ru(phen) can intercalate into the grooves of dsDNA and acts as an ECL indicator; high ECL intensity can therefore be detected from the electrode surface. In the presence of BPA, which can competitively bind with the aptamer owing to their high affinity, Ru(phen) is released from the electrode surface and the ECL of the system is decreased. The decreasing ECL signal has a linear relationship with BPA in the range of 0.1-100 pM with a detection limit of 0.076 pM. The developed biosensor has been applied to detect migration of BPA from different categories of canned drink with satisfactory results.

Electrochemiluminescent functional nucleic acids‐based sensors for food analysis

“…as the ECL probe to construct an ECL aptasensor for BPA. [100] As a result, it showed a LOD of 0.076 × 10 −12 mol/L for BPA. Meanwhile, using this sensor, the migration of BPA in various drinks transferred from the can was investigated.…”

“…Therefore, the determination of BPA is highly important for food safety. Our group used Ru(phen) 3 2+ as the ECL probe to construct an ECL aptasensor for BPA . As a result, it showed a LOD of 0.076 × 10 −12 mol/L for BPA.…”

A paper-analytical device for detecting bisphenol-A in foods

“…At present, a number of assays for BPA detection have been reported, such as gas chromatography, 5 high-performance liquid chromatography, 6 liquid chromatography-mass spectrometry, 7 gas chromatography-mass spectrometry, 8 electrochemical analysis 9,10 and surface-enhanced Raman spectroscopy. 11 Although these methods provide excellent performance in terms of selectivity and sensitivity, they also require expensive and advanced equipment, complex sample preparation and highly trained personnel, all of which are not suitable for real-time and rapid analysis.…”

High-Performance Voltammetric Aptasensing Platform for Ultrasensitive Detection of Bisphenol A as an Environmental Pollutant

“…Aptamers are single-stranded DNAs or RNAs selected by SELEX technology (Systematic Evolution of Ligands by Exponential Enrichment) and can be assembled straightforwardly and are easily accessible (Röthlisberger and Hollenstein, 2018;Villalonga et al, 2020). Additionally, their high specificity and vigorous affinity toward corresponding targets, aptamers can be effortlessly modified through the incorporation of a wide range of functional groups, making them perfectly suitable for a variety of applications (Chen and Yang, 2015;Derikvandi et al, 2016;Ye et al, 2017). Generally, biosensing detection platforms with their substantial specificity and cost-effectiveness offer reliable substitutes to the conventional analytical assays.…”