Highly sensitive capacitive biosensor for detecting white spot syndrome virus in shrimp pond water

Samanman, Saluma; Kanatharana, Proespichaya; Chotigeat, Wilaiwan; Deachamag, Panchalika; Thavarungkul, Panote

doi:10.1016/j.jviromet.2011.01.010

Cited by 33 publications

(12 citation statements)

References 40 publications

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“…Most of the methods for detecting WSSV target vp19 [35], vp28 [22], [26], [28], wsv360 [30], ORF191 [32], [33] and ORF 36 [36]. Among these genes, the vp28 is mostly widely used.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal Recombinase Polymerase Amplification Assay

Xia

Weidmann

et al. 2014

PLoS ONE

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White spot syndrome virus (WSSV) causes large economic losses to the shrimp aquaculture industry, and thus far there are no efficient therapeutic treatments available against this lethal virus. In this study, we present the development of a novel real time isothermal recombinase polymerase amplification (RPA) assay for WSSV detection on a small ESEQuant Tube Scanner device. The RPA sensitivity, specificity and rapidity were evaluated by using a plasmid standard as well as viral and shrimp genomic DNAs. Compared with qPCR, the RPA assay revealed more satisfactory performance. It reached a detection limit up to 10 molecules in 95% of cases as determined by probit analysis of 8 independent experiments within 6.41±0.17 min at 39°C. Consequently, this rapid RPA method has great application potential for field use or point of care diagnostics.

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“…Most of the methods for detecting WSSV target vp19 [35], vp28 [22], [26], [28], wsv360 [30], ORF191 [32], [33] and ORF 36 [36]. Among these genes, the vp28 is mostly widely used.…”

Section: Discussionmentioning

confidence: 99%

Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal Recombinase Polymerase Amplification Assay

Xia

Weidmann

et al. 2014

PLoS ONE

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“…Lane M, low molecular weight standard marker; 1, lysate of bacteria containing pCANTAB-WBP plasmid before induction with 1 mM IPTG; 2, lysate of bacteria containing pCANTAB-WBP plasmid after induction with 1 mM IPTG; and 3, WBP purified protein (3 kDa) concentration that have been demonstrated for the WSSV amount in the range of 1.6 × 10 1 -1.6 × 10 6 copies/μl, with an r value of 0.997, and the detection limit was 1.6 × 10 1 copies/μl. In terms of sensitivity, this immunosensor has less sensitivity compared with a previous work [14]. However, it is good enough since the amount of WSSV in water that is infective and lethal in shrimp was more than 1.6 × 10 4 copies/μl [13].…”

Section: Linear Dynamic Range and Detection Limitmentioning

confidence: 99%

“…The WBP and the anti-GST-VP26 were further developed into an immune-based test assay for detecting the VP26 protein on the envelope of WSSV in shrimp pond water [12,13]. Furthermore, a capacitive immunosensor has been developed for the quantitative detection of WSSV in shrimp pond water during shrimp cultivation [14]. In this study, the polyclonal antibody VP26 mixed WBP was immobilized on the immunosensor surface which both can bind specifically to the VP26 protein of WSSV to increase the sensitivity.…”

Section: Introductionmentioning

confidence: 99%

Application of a Label-Free Immunosensor for White Spot Syndrome Virus (WSSV) in Shrimp Cultivation Water

Waiyapoka

Deachamag

Chotigeat

et al. 2015

Appl Biochem Biotechnol

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White spot syndrome virus (WSSV) is a major pathogen affecting the shrimp industry worldwide. In a preliminary study, WSSV binding protein (WBP) was specifically bound to the VP26 protein of WSSV. Therefore, we have developed the label-free affinity immunosensor using the WBP together with anti-GST-VP26 for quantitative detection of WSSV in shrimp pond water. When the biological molecules were immobilized on a gold electrode to form a self-assembled monolayer, it was then used to detect WSSV using a flow injection system with optimized conditions. Binding between the different copies of WSSV and the immobilized biological molecules was detected by an impedance change (ΔZ″) in real time. The sensitivity of the developed immunosensor was in the linear range of 1.6 × 10(1)-1.6 × 10(6) copies/μl. The system was highly sensitive for the analysis of WSSV as shown by the lack of impedance change when using yellow head virus (YHV). The developed immunosensor could be reused up to 37 times (relative standard deviation (RSD), 3.24 %) with a good reproducibility of residual activity (80-110 %). The immunosensor was simple to operate, reliable, reproducible, and could be applied for the detection and quantification of WSSV in water during shrimp cultivation.

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“…However, they reported that they could not consistently detect WSSV in all fractions/replicates. Recently, Samanman et al (2011) described the use of capacitive biosensor for quantitative detection of WSSV in water. However, this tool is not feasible in most laboratories.…”

Section: Introductionmentioning

confidence: 99%

Tangential flow ultrafiltration for detection of white spot syndrome virus (WSSV) in shrimp pond water

Alavandi

Bharathi

Kumar

et al. 2015

Journal of Virological Methods

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Highly sensitive capacitive biosensor for detecting white spot syndrome virus in shrimp pond water

Cited by 33 publications

References 40 publications

Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal Recombinase Polymerase Amplification Assay

Rapid Detection of Shrimp White Spot Syndrome Virus by Real Time, Isothermal Recombinase Polymerase Amplification Assay

Application of a Label-Free Immunosensor for White Spot Syndrome Virus (WSSV) in Shrimp Cultivation Water

Tangential flow ultrafiltration for detection of white spot syndrome virus (WSSV) in shrimp pond water

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