Highly Sensitive Cobalt Porphyrin Biomimetic Catalyzed Flow Injection Chemiluminescence Determination of Diethylstilbestrol in Pharmaceuticals

Abstract

The great inhibition of diethylstilbestrol to cobalt deuteroporphyrin catalyzed luminol-H2O2 chemiluminescence reaction, has been exploited to develop a novel and sensitive flow-injection chemiluminescence detection method for the determination of diethylstilbestrol. The optimum experimental conditions and the possible mechanism have been investigated. Under the optimized parameters, the relative chemiluminescence intensity is linearly related to the concentration of diethylstilbestrol in the range of 6 × 10 -10 -1.0 × 10 -8 g mL -1 and 1 × 10 -8 -1 × 10 -7 g mL -1 with a detection limit of 3.83 × 10 -10 g mL -1 . The proposed method could be successfully applied to the determination of diethylstilbestrol in pharmaceuticals.