Highly Sensitive Determination of Antibiotic Residues in Aquatic Products by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Ye, Hongli; Li, Siman; Xi, Yinfeng; Shi, Yao; Shang, Xiao-Rui; Huang, Dongmei

doi:10.3390/antibiotics11101427

Cited by 14 publications

(8 citation statements)

References 45 publications

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“…Rapid test kits can be extremely helpful in facilitating the rapid and precise identification of antibiotic residues in certain food products, supporting the government's and relevant stakeholders' efforts to ensure food safety [45,46]. Therefore, it is necessary to develop rapid testing kits for the detection of antibiotic residues in foodstuffs originating from animals and to use them to address this threat to public health [35,[47][48][49][50]. In conclusion, one of the most important aspects of food safety and public health is to keep an eye on antibiotic residues in animal feed.…”

Section: Discussionmentioning

confidence: 99%

Rapid testing of antibiotic residues to increase food safety awareness of animal origin

Widiasih,

Pratama,

Drastini

et al. 2023

Vet World

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Background and Aim: Antibiotics are used to improve growth, reduce disease, and decrease mortality in animals grown for food. The government regulates and prohibits the use of antibiotics, in particular, the use of antibiotic growth promoter (AGP) in livestock; however, it is not yet known whether the use of antibiotics is in accordance with regulations so that there are no antibiotic residues in food of animal origin. To ensure food safety of animal origin and to raise awareness of food safety, it is necessary to detect antibiotic residues in fish, eggs, and chicken meat from Yogyakarta Special Province through monitoring and monitoring. To ensure food safety and regulatory compliance in food samples, antibiotic residue screening techniques are essential. A number of methods, such as time-consuming and costly chromatographic and spectroscopic methods, have been developed for the detection of antibiotic residues in food samples; however, not all laboratories have these facilities. Therefore, a rapid diagnosis of food of animal origin is required. The purpose of this study was to rapidly test antibiotic residues by using Premi®test kits (R-Biopharm AG, Germany) to increase awareness of food safety of animal origin. Materials and Methods: We tested 345 animal-based food samples from traditional markets, supermarkets, and central markets in five districts of Yogyakarta Special Province for antibiotic residues using rapid test kits and observation questionnaires to identify risk factors. Results: The presence of antibiotic residues in food-animal origin samples from the Yogyakarta region had an antibiotic residue level of 9.28% (32/345), consisting of fish samples 11.3% (18/97), eggs 15.65% (1/114), and chicken meat samples 0.87% (13/102). The highest percentage of samples positive for residual antibiotics was 21.9% (7/32) from supermarket meat samples. The highest amounts of antibiotic residues were found in fish samples collected from Sleman Regency, up to 25% (8/32), whereas in supermarket fish samples, there were as high as 18.8% (6/32). Conclusion: Antibiotic residues in animal-based food can be attributed to various factors, including product source, transportation conditions, and environmental conditions. The widespread distribution of antibiotic residues in fish comes from environmental conditions during maintenance, distribution, and retailing. Monitoring antibiotic residue prevalence in food-animal origins, particularly chicken meat, eggs, and fish, is crucial for improving animal food quality and safety. Keywords: antibiotic residual level, antimicrobial resistance, food animal origin, food safety, rapid screening test.

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Section: Discussionmentioning

confidence: 99%

Rapid testing of antibiotic residues to increase food safety awareness of animal origin

Widiasih,

Pratama,

Drastini

et al. 2023

Vet World

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“…LC-MS/MS is the most widely used method for detecting drug residues in food. HPLC-MS/MS technology has gained significant interest due to its quick response time, excellent sensitivity, high accuracy, good separation capability, positive/negative ion mode compatibility and broad applicability in analyzing complex samples like swine, bovine, poultry eggs and chicken muscle, aquatic products, meat and milk [ 14 , 83 , 87 , 96 , 96 ]. The high sensitivity and specificity of MS detectors enable the application of generic sample preparation procedures, allowing for the simultaneous extraction of a wide range of residues with different properties.…”

Section: Analysis Of Drug Residues In Foodmentioning

confidence: 99%

Recent Advances in the Determination of Veterinary Drug Residues in Food

Pratiwi,

Ramadhanti,

Amatulloh

et al. 2023

Foods

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The presence of drug residues in food products has become a growing concern because of the adverse health risks and regulatory implications. Drug residues in food refer to the presence of pharmaceutical compounds or their metabolites in products such as meat, fish, eggs, poultry and ready-to-eat foods, which are intended for human consumption. These residues can come from the use of drugs in the field of veterinary medicine, such as antibiotics, antiparasitic agents, growth promoters and other veterinary drugs given to livestock and aquaculture with the aim of providing them as prophylaxis, therapy and for promoting growth. Various analytical techniques are used for this purpose to control the maximum residue limit. Compliance with the maximum residue limit is very important for food manufacturers according to the Food and Drug Administration (FDA) or European Union (EU) regulations. Effective monitoring and control of drug residues in food requires continuous advances in analytical techniques. Few studies have been reviewed on sample extraction and preparation techniques as well as challenges and future directions for the determination of veterinary drug residues in food. This current review focuses on the overview of regulations, classifications and types of food, as well as the latest analytical methods that have been used in recent years (2020–2023) for the determination of drug residues in food so that appropriate methods and accurate results can be used. The results show that chromatography is still a widely used technique for the determination of drug residue in food. Other approaches have been developed including immunoassay, biosensors, electrophoresis and molecular-based methods. This review provides a new development method that has been used to control veterinary drug residue limit in food.

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“…At present, thin-layer chromatography [ 26 , 27 ], electrochemical methods [ 28 ], fluorescence microscopy [ 29 ], microbial titer analysis [ 30 ], liquid chromatography [ 31 , 32 , 33 , 34 ], gas chromatography-mass spectrometry (GC-MS) [ 35 , 36 ] and liquid chromatography–tandem mass spectrometry (LC-MS) [ 37 , 38 , 39 , 40 ] are the main quantitative methods of analyzing MALs in biological samples. LC-MS, which combines the ultra-performance of liquid chromatography with the high sensitivity of mass spectrometry, has become the preferred method for determining the concentration of drug residues in meat products [ 41 ].…”

Section: Introductionmentioning

confidence: 99%

Dispersive Solid-Phase Extraction and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry—A Rapid and Accurate Method for Detecting 10 Macrolide Residues in Aquatic Products

Chen,

Mei,

Zhang

et al. 2024

Foods

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The amount of macrolide (MAL) residues in aquatic products, including oleandomycin (OLD), erythromycin (ERM), clarithromycin (CLA), azithromycin (AZI), kitasamycin (KIT), josamycin (JOS), spiramycin (SPI), tilmicosin (TIL), tylosin (TYL), and roxithromycin (ROX), was determined using solid-phase extraction and ultra-performance liquid chromatography–tandem mass spectrometry (UPLC-MS/MS). The residues were extracted with 1% ammonia acetonitrile solution and purified by neutral alumina adsorption. Chromatographic separation was completed on an ACQUITY UPLC BEH C18 column with acetonitrile–0.1% formic acid aqueous solution as the mobile phase, and mass spectrometry detection was performed by multiple reaction monitoring scanning with the positive mode in an electrospray ion source (ESI+). Five isotopically labeled compounds were used as internal standards for quality control purposes. The findings indicated that across the mass concentration span of 1.0–100 μg/L, there was a strong linear correlation (R2 > 0.99) between the concentration and instrumental response for the 10 MALs. The limit of detection of UPLC-MS/MS was 0.25–0.50 μg/kg, and the limit of quantitation was 0.5–1.0 μg/kg. The added recovery of blank matrix samples at standard gradient levels (1.0, 5.0, and 50.0 μg/kg) was 83.1–116.6%, and the intra-day precision and inter-day precisions were 3.7 and 13.8%, respectively. The method is simple and fast, with high accuracy and good repeatability, in line with the requirements for accurate qualitative and quantitative analysis of the residues for 10 MALs in aquatic products.

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Highly Sensitive Determination of Antibiotic Residues in Aquatic Products by High-Performance Liquid Chromatography–Tandem Mass Spectrometry

Cited by 14 publications

References 45 publications

Rapid testing of antibiotic residues to increase food safety awareness of animal origin

Rapid testing of antibiotic residues to increase food safety awareness of animal origin

Recent Advances in the Determination of Veterinary Drug Residues in Food

Dispersive Solid-Phase Extraction and Ultra-Performance Liquid Chromatography–Tandem Mass Spectrometry—A Rapid and Accurate Method for Detecting 10 Macrolide Residues in Aquatic Products

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