“…The NOBIAS Chelate PA-1 M chelating resin (NOBIAS resin,150 mg, was used for the pre-concentration of trace metals. The NOBIAS resin has an excellent affinity for metals, out competing natural ligands, as well as the ability to separate alkaline and alkali-earth metals from solution (Minami et al, 2015;Sohrin and Bruland, 2011;Ueda et al, 2010). The resin was initially cleaned with 25 ml of the following solutions passing through in succession at 5 ml/min via a hand syringe: acetone (Wako Pure), methanol (Wako Pure), Milli-Q water, 1 M HCl-0.01 M hydrogen peroxide (ultrapure, Tamapure-AA-10, H2O2, Wako Pure, respectively), Milli-Q water, 0.5 M ammonia (NH4, Tamapure-AA-10), and Milli-Q water following methods outlined in Minami et al (2015).…”