Highly sensitive electrochemical biosensor for bisphenol A detection based on a diazonium-functionalized boron-doped diamond electrode modified with a multi-walled carbon nanotube-tyrosinase hybrid film

Efficient Immobilization of Tyrosinase Enzyme on Layered Double Hydroxide Hybrid Nanomaterials for Electrochemical Detection of Polyphenols

“…However, it is possible to determine a mean molecular mass of polyphenols at 415 g.mol −1 , by taking into account the molar mass of each compound present in the mixture and its relative percentage determined from previous analyses [44]. Based on this estimation, we obtain a linear range of 0-2.4 μM, leading to the conclusion that it is particularly well-adapted to the detection of low concentrations (typical limits of detection and linear ranges usually range from nM to hundreds of μM in the case of tyrosinase-based biosensors [16]- [18], [20]- [25], [27], [28], [63], [65]. In the context of tea polyphenol detection, our maximal concentrations for a linear biosensor response are in agreement with those obtained in tea decoctions, which are around 2-5 μM [17].…”

“…Different approaches have been developed for the immobilization of tyrosinase: silica sol-gel composite films [18], tyrosinase-modified boron-doped diamond electrodes [19], [20], tyrosinase entrapped in an agarose-guar gum [21], glassy carbon electrode modified by gold nanoparticles [22], tyrosinase-FeO 3 nanoparticles-chitosan [23], use of polyaniline-carbon nanofibers or nanotubes [24]- [27], diazonium salt [17] or titania sol-gel matrix [28] … Among host matrixes already used for the immobilization of enzymes recognizing polyphenols [6]- [11], [18]- [30], Layered Double Hydroxide (LDH) nanomaterials are attractive candidates for electrochemical analysis in general and for enzyme immobilization [31], [32], since they exhibit many advantageous features in the development of hybrid biomaterials. LDHs are an unusual family of layered materials consisting of positively charged layers with charge balancing anions inserted between these layers.…”

Practical Application of Aptamer-Based Biosensors in Detection of Low Molecular Weight Pollutants in Water Sources

“…An electrochemical biosensor based on a diazonium-functionalized boron-doped diamond electrode modified with a multi-walled CN-tyrosinase hybrid film was developed for BPA detection. The biosensor displayed a large linear range from 0.01 to 100 nM, with a LOD of 10 pM [ 156 ]. In addition, a reusable evanescent wave immunosensor has been employed for highly sensitive detection of BPA in water samples.…”

High-Performance Voltammetric Aptasensing Platform for Ultrasensitive Detection of Bisphenol A as an Environmental Pollutant

“…As a weak estrogenic compound, BPA interferes in multiple cellular pathways of apoptosis, proliferation, and migration ( Ma et al, 2019 ; Nomiri et al, 2019 ; Rahmani et al, 2020 ). BPA has been shown to cause numerous damaging health effects, including cardiovascular disorders, pregnancy complications, obesity, glucose metabolism disorders and reproductive toxicity ( Kabir et al, 2015 ; Ejaredar et al, 2017 ; Salek-Maghsoudi et al, 2018 ; Rajabnejad et al, 2020 ) even at a concentration as low as tolerable daily intake (TDI) (4 μg/kg/day) ( Zehani et al, 2015 ; Erjavec et al, 2016 ). Although various techniques have already been developed, more cost-effective, robust, and sensitive detection methods have yet to be designed to monitor the level of hazardous chemicals in food and environmental samples ( Hassani et al, 2017 ; Baghayeri et al, 2018 ).…”