Highly Sensitive Electrochemiluminescent Nanobiosensor for the Detection of Palytoxin

Zamolo, Valeria Anna; Valenti, G.; Venturelli, Enrica; Chaloin, Olivier; Marcaccio, Massimo; Boscolo, Sabrina; Castagnola, Valentina; Sosa, Silvio; Berti, Federico; Fontanive, Giampaolo; Poli, Mark; Tubaro, Aurelia; Bianco, Alberto; Paolucci, Francesco; Prato, Maurizio

doi:10.1021/nn302573c

Cited by 103 publications

(52 citation statements)

References 31 publications

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“…Outside the nanoparticle, TPrA, TPrA * + and TPrA * concentrations were determined according to the ECEC mechanism in Scheme 1 for a fully buffered pH of 7.4 (i. e. Eq. (3) being irreversible with k dep ¼ 3:5 Â 10 3 s À1 ) while taking into account diffusion in the solution and the diffusional perturbation created by the nanoparticle without any approximation made, i. e. none of the equations (12)(13)(14)(15)(16)(17)(18)(19) were used. The bulk TPrA concentration was 30 mM and its protonation equilibrium was set at 10 3 .…”

Section: Rolesmentioning

confidence: 99%

“…[10] This is particularly important to avoid undesired background emissions from biological analytes. [11][12][13] Secondly, the excitation system (electrode potential) is decoupled from the detection signal (light) and any potentially transferred electrical noise is filtered by diffusion of the organic reactant intermediate. This leads to very low levels of electrical perturbation of the ECL light intensity that is used as the analytical signal.…”

Section: Introductionmentioning

confidence: 99%

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Theory and Simulation for Optimising Electrogenerated Chemiluminescence from Tris(2,2′‐bipyridine)‐ruthenium(II)‐Doped Silica Nanoparticles and Tripropylamine

et al. 2017

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Electrochemiluminescence (ECL), that is, light emission from an electronically excited species generated by electrochemical means, sustains powerful (bio)analytical methods for the ultrasensitive detection of biological targets. Co-reactant systems involving an inexpensive organic molecule, most generally a tertiary amine, and a metal complex luminophore are commonly used for such purposes. Owing to the high cost of the luminophore moiety, several groups have considered minimising its quantity by sequestrating it at high concentration inside nanoparticles. However, to be efficient and to optimise ECL responses, this strategy requires that the nanoparticle carrier is suitably placed inside the diffusion layer of the oxidised organic co-reactant. In this work, we firstly investigated this optimisation problem by introducing a rather simple analytical model to delineate qualitatively the main mechanistic features controlling the ECL intensity. This was then analysed in more detail by using 2D simulations. Analysis of these 2D-heavy simulations in terms of memory occupation and CPU time, evidenced that similar results (i. e. with a relative precision best than a few percent) could be achieved with much faster 1D simulations. These 1D simulations allowed specifying quantitatively the main features of the analytical model qualitative predictions and to propose simple rules for the optimisation of the luminophoredoped nanoparticles placement inside the diffusion layer of the organic co-reactant.

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Section: Rolesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Theory and Simulation for Optimising Electrogenerated Chemiluminescence from Tris(2,2′‐bipyridine)‐ruthenium(II)‐Doped Silica Nanoparticles and Tripropylamine

et al. 2017

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“…A novel sandwich elISA for detection of Pltx was introduced and also evaluated, providing a lOQ of 11 ng Pltx/ml mussel extract, 9.6 ng Pltx/ml algae sample and 2.4 ng Pltx/ml for seawater samples (Boscolo et al, 2013). Via introduction of an electrochemiluminescence-based sensor a lOQ of 2.2 µg Pltx kg of shellfish tissue was achieved, which is in the area -if not lower -than the lOQ of some of the lC-MS/MS approaches (Zamolo et al, 2012). Recently also a novel surface plasmon resonance immunoassay was introduced with an instrumental limit of detection for Pltx in sub-ng/ml sample range (Yakes et al, 2011).…”

Section: )mentioning

confidence: 99%

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Daneshian

2013

ALTEX

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Summary Aquatic food accounts for over 40% of global animal food products, and the potential contamination with toxins of algal origin -marine biotoxins -poses a health threat for consumers. The gold standards to assess toxins in aquatic food have traditionally been in vivo methodssingle marine biotoxins or combinations of marine biotoxins and intoxication symptomatology as well as monitoring the "success" of controls implemented during fishery and aquaculture production, processing, and retailing. For this reason, a workshop was organized in ermatingen, Switzerland by the Center for Alternatives to Animal testing -europe (CAAteurope) within the framework of the transatlantic think tank for toxicology (t 4 ).In contrast to other food products where hygiene and potential contamination with microbes or mold toxins is the prime issue, the emphasis in aquatic products (shellfish and finfish) is, beyond viral and bacterial contaminations, primarily placed on potential contamination with marine biotoxins owing to the numerous and recurring human intoxications.The gold standards to assess toxins in aquatic food have traditionally been in vivo methods, i.e., the mouse and the rat bioassays. Besides the ethical issues of in vivo bioassays there are specific difficulties, e.g., different exposure route (i.p. versus oral), low inter-species comparability, high intra-species variability, and questionable extrapolation of quantitative risk to humans, thus highlighting the need for the development of more reliable detection and quantification methods. Based on this reasoning, the european Food Safety Authority (eFSA) advocates the use of an analytical method, i.e., LC-MS (Liquid Chromatography coupled Mass Spectrometry), as a substitute for in vivo bioassays for almost all classes of marine toxins. However, although lC-MS is a very sensitive method that has the advantages of being able to detect multiple toxins in a single analysis and being good for confirming the identity of toxins, the quality of quantitative analysis is dependent on the availability of calibration standards. While many new toxin structural analogues have been detected and identified using LC-MS, this technique -as with most other methods -is not good at detecting new toxin types. When new toxin analogues are detected but accurate standards are not yet available, only an approximate quantitation is possible using estimated response factors.For the purpose of risk assessment of food, however, it is imperative to focus on the possible adverse effects, independent of whether they stem from one toxin or a combination of toxins. As toxicity is defined by functional and structural changes of biological systems, the development of a human-relevant in vitro system for appropriate risk assessment of marine biotoxins in seafood stands to reason. Moreover, poor correlation of human intoxications, of acute symptomatology and long-term adverse effects, and of predictive in vitro, in vivo, and analytical detection methodologies of marine biotoxins stems not least from a...

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“…The presented biosensor is robust, cost-effective, and simple to use, avoiding the pitfalls of traditional screening methods by directly specifying the identity of the toxic substance. Zamolo et al (2012) developed an ultrasensitive electrochemiluminescencebased sensor for the detection of palitoxin (PlTX), one of the most potent marine toxins, frequently detected in seafood, taking advantage of the specificity provided by anti-PlTX antibodies, the good conductive properties of carbon nanotubes, and the excellent sensitivity achieved by a luminescence-based transducer. The sensor was able to produce a concentration-dependent light signal, allowing PlTX quantification in mussels, with a limit of detection of 2.2 μg/kg of mussel meat), more than two orders of magnitude more sensitive than that of the commonly used detection techniques, such as LC-MS/MS.…”

Section: Biosensors For Bacteria and Bacteria Toxins Detectionmentioning

confidence: 99%

Recent Biosensors for Food Analysis in Brazil and Italy

Pedrosa

Fleuri

Lima

et al. 2013

Food Quality, Safety and Technology

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The importance of safe and high-quality food products is doubtless and consumer demand for increased food quality and safety assurances moves down the chain with retailers and service providers asking suppliers and producers to provide verifiable proof that robust food quality and safety control systems have been effectively implemented. Food analysis needs rapid and reliable methods to ensure the quality of products and process control. Food quality control is essential both for consumer protection and also for food industry. Nowadays, the convergence of new technologies, including biotechnology, nanotechnology, and electronic technology, has opened new horizons in development of biosensors. These devices offer advantages as alternatives to conventional methods because they enable real-time detection, portability, and fast laboratory or in-field analysis. This contribution presents a review about the development and application of biosensor technology in foods, and future trends in Brazil and Italy.

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Highly Sensitive Electrochemiluminescent Nanobiosensor for the Detection of Palytoxin

Cited by 103 publications

References 31 publications

Theory and Simulation for Optimising Electrogenerated Chemiluminescence from Tris(2,2′‐bipyridine)‐ruthenium(II)‐Doped Silica Nanoparticles and Tripropylamine

Theory and Simulation for Optimising Electrogenerated Chemiluminescence from Tris(2,2′‐bipyridine)‐ruthenium(II)‐Doped Silica Nanoparticles and Tripropylamine

A roadmap for hazard monitoring and risk assessment of marine biotoxins on the basis of chemical and biological test systems

Recent Biosensors for Food Analysis in Brazil and Italy

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