Highly sensitive enzyme immunoassay for human creatine kinase BB isozyme

Kato, Kanefusa; Shimizu, Atsuko; Ishiguro, Yukio; Mokuno, Kenji; Ariyoshi, Y; Nakajima, Takashi

doi:10.1016/0009-8981(85)90308-0

Cited by 33 publications

(22 citation statements)

References 23 publications

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“…[A.51 not only is tailor-made for this particular assay but also is applicable to most sandwich assays based on the same principle, e.g., ELISA and IRMA. We tested this for some recently published assays in which the dose-response curves (21)(22)(23)(24)(25)(26) were shown. Five out of six different dose-response curves resulted in a better fit using Eq.…”

Section: Discussiopjmentioning

confidence: 99%

A three-parameter langmuir-type model for fitting standard curves of sandwich enzyme immunoassays with special attention to the α-fetoprotein assay

Kortlandt

Endeman

Hoeke

1987

Analytical Biochemistry

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In a simplified approach to the reaction kinetics of enzyme-linked immunoassays, a Langmuir-type equation y = [ux/(b + x)] + c was derived. This model proved to be superior to logit-log and semilog models in the curve-fitting of standard curves. An assay for a-fetoprotein developed in our laboratory with a sensitivity of 2 g/liter and a between-day coefficient of variation of about 15% was used for comparison of the different models. The proposed model has the advantage that it is based on the law of mass action and that blank determinations can be included in the calculation of the curves without giving a disproportionate bias. o 1987 Academic Press, Inc.

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Section: Discussiopjmentioning

confidence: 99%

A three-parameter langmuir-type model for fitting standard curves of sandwich enzyme immunoassays with special attention to the α-fetoprotein assay

Kortlandt

Endeman

Hoeke

1987

Analytical Biochemistry

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“…The specific antibody immunoglobulin G (IgG) was purified from the antisera by use of antigen-coupled Sepharose column as decribed for the pUI'lf1CE1tlO1'1'Of antibodies to human CK-BB (8). The antibodies used for the preparation of immunoassay were digested with pepsin from porcine stomach mucosa (Sigma) to obtain the F(ab')2 fragment of the antibodies (3,5).…”

Section: Antibodiesmentioning

confidence: 99%

“…The assay system, composed of polystyrene balls with immobilized antibody F(ab')2 fragments (6) and the antibody Fab' fragments labeled with ,6-D-galactosidase from Escherichia coii (3,5), was prepared as described previously for the assay system of human CK-BB (8). The polystyrene balls with immobilized antibodies and the galactosidase-labeled antibodies were stored at 4°C in 0.01 M sodium phosphate buffer, pH 7.0 containing 0.1 M NaCl, l mM MgCl2, 0.1% bovine serum albu-K. KATO and A. SHIMIZU min (fraction V, Armour Pharmaceutical, IL), and 0.l% NaN3 (buffer A).…”

Section: Immunoassay Systemmentioning

confidence: 99%

“…Recently, we developed a highly sensitive immunoassay system for separate measurement of three forms of human CK and estimated the contents in various tissues (7,8). However, because of difficulty in obtaining fresh human tissue samples, the values determined were supposed to be lower than the i actual contents.…”

mentioning

confidence: 99%

“…Since the cross-reactivities of the human CK assay system with rat or dog CK isozymes were low, the assay method was not applicable to the study with experimental animals. In order to obtain more precise data on the pathological significance of serum CK isozymes, including those on the tissue-distribution of CK isozymes, we now developed an immunoassay system for rat CK isozymes, which is similar to that for human CK reported previously (7,8). A preliminary part of the present work has been published (9).…”

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confidence: 99%

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<b>ENZYME IMMUNOASSAY SYSTEM FOR MEASUREMENT OF THREE FORMS (MM, MB AND BB) OF RAT CREATINE </b><b>KINASE </b>

Kato¹,

Shimizu²

1987

Biomed. Res.

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A highly sensitive sandwich-type enzyme immunoassay system for the assay of rat creatine kinase (CK) MM, MB and BB isozymes was developed by use of [5'-D-galactosidase from Escherichia coir' as label and the purified antibodies raised in rabbits by injecting CK-MM or CK-BB. The antibodies specific to each subunit were purified from the immunoglobulin G (lgG) fractions of antisera by immunoaffinity chromatography with a column of respective antigen (CK-MM or CK-BB)-coupled Sepharose. The CK-MM and CK-BB assays were performed with the respective antibody F(ab')2 fragments-immobilized polystyrene balls and the same antibody Fab' fragments labeled with ,6'-D-galactosidase from Escherichia c0h'. The CK-MB was assayed with the polystyrene balls with either antibody (anti-CK-M or anti-CK-B) and another antibody (anti-CK-B and anti-CK-M, respectively) labeled with galactosidase. The minimum detection limit of each assay was 1 to 10 pg or (l0 to 100 amol) per tube. The assays for CK-MM and CK-BB were cross-reacted about l2°/0 with CK-MB, but the assay for CK-MB was specific showing no cross-reactivity with and no interference by CK-MM and CK-BB under the limited conditions. Therefore, the concentrations of CK-MM and CK-BB were able to estimate by subtracting the cross-reacting CK-MB in the samples. The concentrations of three forms of CK in various rat tissues were determined by the present assay system. High concentrations of CK-MM were observed in the heart and tissues composed of striated muscle or chondrocytes. CK-MB was also present at relatively high levels in the above-mentioned tissues, while CK-BB was mostly located in the central nervous system and tissues composed of smooth muscle. These results are in line with the previous reports on the distribution of CK isozymes in human tissues.

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The diagnostic and prognostic value of pretreatment serum creatine kinase bb levels in patients with neuroblastoma

Ishiguro

Kato

Akatsuka

et al. 1990

Cancer

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The serum levels of the BB isozyme of creatine kinase (CK-BB) and neuron-specific enolase (NSE) were measured before therapy in 35 patients with neuroblastoma. Sixty percent (21 of 35) of neuroblastoma patients had CK-BB levels higher than 11 ng/ml. The extent of disease was associated with an increased incidence of elevated serum CK-BB levels. The highest pretreatment serum CK-BB titers were found in patients with Stage IV disease. A strong correlation between the pretreatment CK-BB level and the outcome in patients with neuroblastoma was observed. Eleven (79%) of 12 patients who had a serum CK-BB level greater than 15 ng/ml died, and eight of ten (80%) who had a serum level less than 11 ng/ml were alive and tumor free after 2 years. A positive linear correlation between the pretreatment CK-BB and NSE (n = 35, r = 0.695) levels was found.

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Highly sensitive enzyme immunoassay for human creatine kinase BB isozyme

Cited by 33 publications

References 23 publications

A three-parameter langmuir-type model for fitting standard curves of sandwich enzyme immunoassays with special attention to the α-fetoprotein assay

A three-parameter langmuir-type model for fitting standard curves of sandwich enzyme immunoassays with special attention to the α-fetoprotein assay

<b>ENZYME IMMUNOASSAY SYSTEM FOR MEASUREMENT OF THREE FORMS (MM, MB AND BB) OF RAT CREATINE </b><b>KINASE </b>

The diagnostic and prognostic value of pretreatment serum creatine kinase bb levels in patients with neuroblastoma

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