Highly sensitive gas chromatographic—mass spectrometric screening method for the determination of picogram levels of fentanyl, sufentanil and alfentanil and their major metabolites in urine of opioid exposed workers

Nimmen, Nadine F.J Van; Poels, Katrien; Veulemans, Hendrik

doi:10.1016/j.jchromb.2004.01.044

Cited by 58 publications

(39 citation statements)

References 33 publications

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“…Although medically beneficial to patients, because of their high potency, synthetic opioid narcotics pose a problem for serious healthrelated injuries in an occupational setting if the exposure to these agents is not controlled. Therefore, Van Nimmen et al (2004) have developed a highly sensitive assay that would determine trace levels of several opioid narcotics such as fentanyl, sufentanil and alfentanil and their corresponding metabolites in urine matrix. The objective of developing this assay was to obtain a fast, high-throughput procedure with high sensitiivty that would be easily adoptable as a screening assay.…”

Section: Drug Monitoring For Therapeutic Benefits or Occupational Hazardmentioning

confidence: 99%

Applicability of bioanalysis of multiple analytes in drug discovery and development: review of select case studies including assay development considerations

Srinivas

2006

Biomed. Chromatogr.

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The development of sound bioanalytical method(s) is of paramount importance during the process of drug discovery and development culminating in a marketing approval. Although the bioanalytical procedure(s) originally developed during the discovery stage may not necessarily be fit to support the drug development scenario, they may be suitably modified and validated, as deemed necessary. Several reviews have appeared over the years describing analytical approaches including various techniques, detection systems, automation tools that are available for an effective separation, enhanced selectivity and sensitivity for quantitation of many analytes. The intention of this review is to cover various key areas where analytical method development becomes necessary during different stages of drug discovery research and development process. The key areas covered in this article with relevant case studies include: (a) simultaneous assay for parent compound and metabolites that are purported to display pharmacological activity; (b) bioanalytical procedures for determination of multiple drugs in combating a disease; (c) analytical measurement of chirality aspects in the pharmacokinetics, metabolism and biotransformation investigations; (d) drug monitoring for therapeutic benefits and/or occupational hazard; (e) analysis of drugs from complex and/or less frequently used matrices; (f) analytical determination during in vitro experiments (metabolism and permeability related) and in situ intestinal perfusion experiments; (g) determination of a major metabolite as a surrogate for the parent molecule; (h) analytical approaches for universal determination of CYP450 probe substrates and metabolites; (i) analytical applicability to prodrug evaluations-simultaneous determination of prodrug, parent and metabolites; (j) quantitative determination of parent compound and/or phase II metabolite(s) via direct or indirect approaches; (k) applicability in analysis of multiple compounds in select disease areas and/or in clinically important drug-drug interaction studies. A tabular representation of select examples of analysis is provided covering areas of separation conditions, validation aspects and applicable conclusion. A limited discussion is provided on relevant aspects of the need for developing bioanalytical procedures for speedy drug discovery and development. Additionally, some key elements such as internal standard selection, likely issues of mass detection, matrix effect, chiral aspects etc. are provided for consideration during method development.

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Section: Drug Monitoring For Therapeutic Benefits or Occupational Hazardmentioning

confidence: 99%

Applicability of bioanalysis of multiple analytes in drug discovery and development: review of select case studies including assay development considerations

Srinivas

2006

Biomed. Chromatogr.

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“…The use of high purity solvents and disposable extraction vials and a dedicated cleaning procedure for other materials aided in avoiding contamination. Other precautions are discussed in length elsewhere [11]. Briefly, they included the addition of a small percentage of polyethylene glycol (0.01% (v/v)) to the extraction solvent and the use of freshly silanized GC inlet Table 2 Linear regression models (Y = ax + b) for the opioid narcotics for concentrations ranging from 0.1 to 2 times their individual occupational exposure limit (OEL-TWA for a 960 l air sample) and surface contamination limits (SCL) Slope (95% C.I.…”

Section: Limits Of Detection (Lods) and Limits Of Quantification (Loqs)mentioning

confidence: 99%

“…This research is presented in detail elsewhere [11]. MN 200), toluene (spectrophotometric grade) and acetone (HPLC grade) were obtained from Sigma-Aldrich (Steinheim, Germany).…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a highly sensitive gas chromatographic–mass spectrometric screening method for the simultaneous determination of nanogram levels of fentanyl, sufentanil and alfentanil in air and surface contamination wipes

Nimmen

Veulemans

2004

Journal of Chromatography A

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“…Highly sensitive methods of analysis for fentanyl have been established with gas chromatography (GC) and high-performance liquid chromatography (HPLC), especially in human plasma, hair or urine [5][6][7][8][9][10][11][12][13][14][15][16][17][18][19] . Nevertheless, very few methods of the degradation of fentanyl are reported in literatures.…”

Section: Introductionmentioning

confidence: 99%

Oxidative Degradation of Fentanyl in Aqueous Solutions of Peroxides and Hypochlorites

Qi¹,

Cheng²,

Zuo³

et al. 2011

DSJ

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Fentanyl widely used in clinics as practices is potentially utilised as an incapacitant in countering terrorism and its analogues would be used as an addictive drug, so the degradation of these compounds need to be investigated for protecting environment and human health. In this work, the degradation of fentanyl was examined in a series of oxidant aqueous solutions. Meanwhile, the degradation pathways of fentanyl in the oxidant solution were discussed according to all products identified by GC/MS and LC/MS.

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Highly sensitive gas chromatographic—mass spectrometric screening method for the determination of picogram levels of fentanyl, sufentanil and alfentanil and their major metabolites in urine of opioid exposed workers

Cited by 58 publications

References 33 publications

Applicability of bioanalysis of multiple analytes in drug discovery and development: review of select case studies including assay development considerations

Applicability of bioanalysis of multiple analytes in drug discovery and development: review of select case studies including assay development considerations

Development and validation of a highly sensitive gas chromatographic–mass spectrometric screening method for the simultaneous determination of nanogram levels of fentanyl, sufentanil and alfentanil in air and surface contamination wipes

Oxidative Degradation of Fentanyl in Aqueous Solutions of Peroxides and Hypochlorites

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