Highly Sensitive HPLC Method for Estimation of Total or Individual Curcuminoids in &lt;i&gt;Curcuma&lt;/i&gt; Cultivars and Commercial Turmeric Powders

Kulyal, Poonam; Kuchibhatla, Lalitha N.; Maheshwari, K. Uma; Babu, K. Nirmal; Tetali, Sarada D.; Raghavendra, Agepati S.

doi:10.18520/cs/v111/i11/1816-1824

Cited by 11 publications

(8 citation statements)

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“…In one of our previous studies, we reported that the HPLC method could be a valuable tool to differentiate the cultivars of Curcuma spp. based on their curcuminoids content ratios ( Kulyal et al, 2016 ). Curcuminoids play a significant role in food, cosmetics, and medicinal compounds.…”

Section: Discussionmentioning

confidence: 99%

“…are dependent on their chemical profiles, studies on the chemical constituents of turmeric/wild turmeric and their essential oils gained significance. Thin-layer chromatography (TLC) is one of the methods employed to quantify curcumin ( Setyaningsih et al, 2016 ) and other curcuminoids ( Phattanawasin et al, 2009 ) in Curcuma longa L. A few different techniques used were HPTLC ( Pathania et al, 2006 ; Paramasivam et al, 2009 ), nuclear magnetic resonance (NMR) spectroscopy ( Li W. et al, 2009 ), and the HPLC method ( Kulyal et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

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Variable Secondary Metabolite Profiles Across Cultivars of Curcuma longa L. and C. aromatica Salisb.

et al. 2021

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Background:Curcuma spp. (Zingiberaceae) are used as a spice and coloring agent. Their rhizomes and essential oils are known for medicinal properties, besides their use in the flavoring and cosmetic industry. Most of these biological activities were attributed to volatile and nonvolatile secondary metabolites present in the rhizomes of Curcuma spp. The metabolite variations among the species and even cultivars need to be established for optimized use of Curcuma spp.Objectives: We compared the phytochemical profiles of rhizomes and their essential oils to establish the variability among seven cultivars: five of Curcuma longa L. (Alleppey Supreme, Duggirala Red, Prathibha, Salem, Suguna) and two of C. aromatica Salisb. (Kasturi Araku, Kasturi Avidi). The GC-MS and LC-MS-based analyses were employed to profile secondary metabolites of these selected cultivars.Methods: Rhizomes of Curcuma spp. were subjected to hydro-distillation to collect essential oil and analyzed by GC-MS. The methanol extracts of fresh rhizomes were subjected to LC-MS analyses. The compounds were identified by using the relevant MS library databases as many compounds as possible.Results: The essential oil content of the cultivars was in the range of 0.74–1.62%. Several compounds were detected from the essential oils and rhizome extracts by GC-MS and LC-MS, respectively. Of these, 28 compounds (13 from GCMS and 15 from LCMS) were common in all seven cultivars, e.g., α-thujene, and diarylheptanoids like curcumin. Furthermore, a total of 39 new compounds were identified from C. longa L. and/or C. aromatica Salisb., most of them being cultivar-specific. Of these compounds, 35 were detected by GC-MS analyses of essential oils, 1,2-cyclohexanediol, 1-methyl-4-(1-methylethyl)-, and santolina alcohol, to name a few. The other four compounds were detected by LC-MS of the methanolic extracts of the rhizomes, e.g., kaempferol-3,7-O-dimethyl ether and 5,7,8-trihydroxy-2′,5′-dimethoxy-3′,4′-methylene dioxyisoflavanone.Conclusions: We identified and recorded the variability in the metabolite profiles of essential oils and whole rhizome extracts from the seven cultivars of Curcuma longa L. and C. aromatica Salisb. As many as 39 new metabolites were detected in these seven Indian cultivars of Curcuma spp. Many of these compounds have health benefits.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Variable Secondary Metabolite Profiles Across Cultivars of Curcuma longa L. and C. aromatica Salisb.

et al. 2021

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“…For instance, Kulyal and coworkers applied principal component analysis (PCA) to the characterization of turmeric cultivars. Authors concluded that curcuminoid levels could be used to assess the turmeric quality and to discriminate among the plant varieties [28]. In another study, gas chromatography-MS (GC-MS) and LC-MS platforms were used to obtain the compositional profiles of curcuminoids and terpenoids as the data for PCA and cluster analysis.…”

Section: Introductionmentioning

confidence: 99%

Determination of Curcuminoids by Liquid Chromatography with Diode Array Detection: Application to the Characterization of Turmeric and Curry Samples

Vidal

Castilla

Aliaga‐Alcalde

et al. 2020

CAC

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Background: A simple, rapid and efficient method for the determination of curcumin and other polyphenols in turmeric and curry samples was here developed. The method relied on sample extraction with methanol and extract analysis by liquid chromatography with diode array detection (HPLC-DAD). Methods: The separation of components was carried out in reversed-phase mode using an elution gradient based on 0.1% (v/v) formic acid aqueous solution and acetonitrile as the components of the mobile phase. Chromatograms were recorded at 420 nm for specific monitoring of curcumin and related compounds. Results: Extraction and separation conditions were optimized by experimental design and multicriteria response functions. Figures of merit were established under the selected experimental conditions. In general, repeatability of peak areas were better than 0.4%, detection limits were below 0.006 mg L-1 and quantitative recoveries expressed as a percentage were about 100 ± 2. The method was applied to quantify curcuminoids in commercial samples. It was found that apart from curcumin, demethoxycurcumin and bisdemethoxycurcumin, other related molecules also occurred in the samples. In this regard, a tentative elucidation of possible unknown curcuminoids was attempted by liquid chromatography coupled to mass spectrometry. Conclusion: Differences in the compositional profiles among samples were encountered to be relevant, so that the resulting HPLC-DAD data was exploited for chemometric characterization of turmeric and curry samples. Samples were successfully discriminated according to matrix types, species varieties and origins.

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“…Gas chromatography coupled to mass spectrometry (GC–MS) has also been used for the characterization of powdered turmeric [ 32 ] and turmeric oil [ 33 ] samples. Nevertheless, liquid chromatography (LC) with UV detection (LC-UV) [ 34 , 35 , 36 , 37 , 38 , 39 , 40 ] and coupled to mass spectrometry (LC–MS) [ 32 , 41 ] are currently the techniques of choice. Besides, the great chemical diversity among bioactive substances, including polyphenolic and curcuminoid compounds, and the wide range of concentrations in which these phytochemicals can be found in turmeric and related products make liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) the most appropriate strategy for characterization, identification and authentication purposes [ 42 ].…”

Section: Introductionmentioning

confidence: 99%

Characterization, Classification and Authentication of Turmeric and Curry Samples by Targeted LC-HRMS Polyphenolic and Curcuminoid Profiling and Chemometrics

et al. 2020

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The importance of monitoring bioactive substances as food features to address sample classification and authentication is increasing. In this work, targeted liquid chromatography coupled to high-resolution mass spectrometry (LC-HRMS) polyphenolic and curcuminoid profiles were evaluated as chemical descriptors to deal with the characterization and classification of turmeric and curry samples. The profiles corresponding to bioactive substances were obtained by TraceFinderTM software using accurate mass databases with 53 and 24 polyphenolic and curcuminoid related compounds, respectively. For that purpose, 21 turmeric and 9 curry samples commercially available were analyzed in triplicate by a simple liquid–solid extraction procedure using dimethyl sulfoxide as extracting solvent. The obtained results demonstrate that the proposed profiles were excellent chemical descriptors for sample characterization and classification by principal component analysis (PCA) and partial least squares-discriminant analysis (PLS-DA), achieving 100% classification rates. Curcuminoids and some specific phenolic acids such as trans-cinnamic, ferulic and sinapic acids, helped on the discrimination of turmeric samples; polyphenols, in general, were responsible for the curry sample distinction. Besides, the combination of both polyphenolic and curcuminoid profiles was necessary for the simultaneous characterization and classification of turmeric and curry samples. Discrimination among turmeric species such as Curcuma longa vs. Curcuma zedoaria, as well as among different Curcuma longa varieties (Alleppey, Madras and Erode) was also accomplished.

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Highly Sensitive HPLC Method for Estimation of Total or Individual Curcuminoids in <i>Curcuma</i> Cultivars and Commercial Turmeric Powders

Cited by 11 publications

References 19 publications

Variable Secondary Metabolite Profiles Across Cultivars of Curcuma longa L. and C. aromatica Salisb.

Variable Secondary Metabolite Profiles Across Cultivars of Curcuma longa L. and C. aromatica Salisb.

Determination of Curcuminoids by Liquid Chromatography with Diode Array Detection: Application to the Characterization of Turmeric and Curry Samples

Characterization, Classification and Authentication of Turmeric and Curry Samples by Targeted LC-HRMS Polyphenolic and Curcuminoid Profiling and Chemometrics

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