Highly sensitive immunoassay based on Raman reporter-labeled immuno-Au aggregates and SERS-active immune substrate

Song, Chunyuan; Wang, Zhuyuan; Zhang, Ruohu; Yang, Jing; Tan, Xuebin; Cui, Yiping

doi:10.1016/j.bios.2009.08.035

Cited by 137 publications

(81 citation statements)

References 35 publications

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“…Finally, hot junctions emerge from the edges between nanoparticles and the plane well substrate. Recently, Song et al reported that the immunoassay carried out on a gold substrate is more sensitive than that based on a glass substrate (Braun et al, 2007;Grubisha et al, 2003;Kim and Yoon, 2005;Song et al, 2009;Yoon et al, 2009). Gold patterned array, used in this work, has a "smooth" film structure and it produces only very low levels of SERS enhancement.…”

Section: Resultsmentioning

confidence: 94%

“…Recently, a surface-enhanced Raman scattering (SERS)-based detection technique has been considered as a promising alternative for highly sensitive detection in ELISA microarrays (Lim et al, 2010;Wang et al, 2010;Song et al, 2009;Bao et al, 2009;Stevenson et al, 2009;Li et al, 2008Li et al, , 2009Abell et al, 2009;Kim et al, 2009;Das et al, 2009;Lin et al, 2008;Fang et al, 2008). Typically, this technique also utilizes the protocol of sandwich immunocomplex assay.…”

Section: Introductionmentioning

confidence: 97%

See 1 more Smart Citation

Highly reproducible immunoassay of cancer markers on a gold-patterned microarray chip using surface-enhanced Raman scattering imaging

Lee

et al. 2011

Biosensors and Bioelectronics

136

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Section: Resultsmentioning

confidence: 94%

Section: Introductionmentioning

confidence: 97%

Highly reproducible immunoassay of cancer markers on a gold-patterned microarray chip using surface-enhanced Raman scattering imaging

Lee

et al. 2011

Biosensors and Bioelectronics

136

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“…After addition of a certain amount of 4-MBA, the intensity of the Au/Ag core-shell nanoparticle absorption band reduced markedly and the absorption peak red-shifted to 404 nm (red curve), suggesting that the monodispersed core-shell nanoparticles aggregated slightly. 24 However, no new plasma resonance absorption peak appeared in the longer red/near infrared wavelength region, indicating that 4 µL of 4-MBA did not induce heavy colloid aggregation and severe sedimentation. SERS spectra were measured to validate the SERS activity of different substrates.…”

Section: Characterization Of Lmp1-sers Nanoparticlesmentioning

confidence: 91%

“…18,28 The degree of aggregation depends greatly on the amount of Raman label added. 24 Because of the strong adsorbability of the thiol group on SERS probe molecules such as 4-MBA, the double electronic layer around the colloid particle surface coated by citrate would be destroyed and lead to instability and aggregation of colloid particles. Consequently, agglomeration would reduce the accuracy of SERS measurement.…”

Section: Optimum Quantity Of 4-mbamentioning

confidence: 99%

Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering

Chen

Zheng²,

Chen³

et al. 2011

IJN

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Background:Previous studies have shown that Epstein-Barr virus (EBV)-encoded latent membrane protein 1 (LMP1) is closely associated with the occurrence and development of nasopharyngeal carcinoma, and can be used as a tumor marker in screening for the disease. Here we report a new methodology based on highly specific and sensitive surface-enhanced Raman scattering (SERS) technology to detect LMP1 in nasopharyngeal tissue sections directly with no need of tedious procedures as with conventional immunohistochemistry methods. Methods: LMP1-functionalized 4-mercaptobenzoic acid (4-MBA)-labeled Au/Ag core-shell bimetallic nanoparticles were prepared first and then applied for analyzing LMP1 in formalinfixed paraffin-embedded nasopharyngeal tissue sections obtained from 34 cancer patients and 20 healthy controls. SERS spectra were acquired from a 25 × 25 spot square area on each tissue section and used to generate SERS images. Results: Data from SERS spectra and images show that this new SERS-based immunoassay detected LMP1 in formalin-fixed paraffin-embedded nasopharyngeal tissue sections with high sensitivity and specificity. The results from the new LMP1-SERS probe method are superior to those of conventional immunohistochemistry staining for LMP1, and in excellent agreement with those of in situ hybridization for EBV-encoded small RNA (EBER). Conclusion:This new SERS technique has the potential to be developed into a new clinical tool for detection and differential diagnosis of nasopharyngeal carcinoma as well as for predicting metastasis and immune-targeted treatment of nasopharyngeal carcinoma.

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“…They are considered to have many advantages over traditional enzyme linked immunosorbent assays (ELISAS) which are considered tedious, time consuming, and rely on the use of toxic and unstable fluorescent dyes and quantum dots (QDs) [282,[315][316][317]. It is known that SERS has equal or in some cases a greater enhancement of Raman signal when compared to fluorescent markers and that SERS metal substrates produce spectral line widths which are 10-100 times narrower compared to fluorescence [64,318,319].…”

Section: Sers Immunoassaysmentioning

confidence: 99%

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

2016

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By electrodeposition and galvanic replacement reaction, we developed a facile, time-saving, cost-effective, and environmentally friendly, two-step synthesis route to obtain a controllable cobalt oxide/Au hierarchically nanostructured electrode for glucose sensing. The nanomaterials were characterized by transmission electron microscopy, scanning electron microscopy, Raman spectroscopy, energy-dispersive spectrometry, and X-ray photoelectron spectroscopy, meanwhile, the sensing performance was investigated by cyclic voltammetry and amperometric response. The results revealed that this novel electrode exhibited excellent electrocatalytic performance toward glucose oxidation, with a wide double-linear range from 0.2 μM to 20 mM and a low detection limit of 0.1 μM based on a signal-to-noise ratio of 3, which was mainly attributed to the ability of loading a small amount of Au with good electron conductivity on the surface of cobalt oxide nanosheets with large active surface area and synergistic electrocatalytic activity of Au and cobalt oxide toward glucose electrooxidation. This facile, sensitive, and selective glucose sensor is also proven to be suitable for the detection of glucose in human serum.

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Highly sensitive immunoassay based on Raman reporter-labeled immuno-Au aggregates and SERS-active immune substrate

Cited by 137 publications

References 35 publications

Highly reproducible immunoassay of cancer markers on a gold-patterned microarray chip using surface-enhanced Raman scattering imaging

Highly reproducible immunoassay of cancer markers on a gold-patterned microarray chip using surface-enhanced Raman scattering imaging

Immunoassay for LMP1 in nasopharyngeal tissue based on surface-enhanced Raman scattering

Controllable Cobalt Oxide/Au Hierarchically Nanostructured Electrode for Nonenzymatic Glucose Sensing

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