Highly-sensitive label-free electrochemical carcinoembryonic antigen immunosensor based on a novel Au nanoparticles–graphene–chitosan nanocomposite cryogel electrode

Samanman, Saluma; Numnuam, Apon; Limbut, Warakorn; Kanatharana, Proespichaya; Thavarungkul, Panote

doi:10.1016/j.aca.2014.10.006

Cited by 81 publications

(24 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1.0×10 -6 to 1.0 ng mL -1 (2.0×10 -7 ng mL -1 ) (Samanman et al, 2015) The results obtained from serum samples are in agreement with enzyme linked fluorescent assay.…”

Section: References and Other Remarkssupporting

confidence: 71%

Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Luong

Vashist

2017

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…1.0×10 -6 to 1.0 ng mL -1 (2.0×10 -7 ng mL -1 ) (Samanman et al, 2015) The results obtained from serum samples are in agreement with enzyme linked fluorescent assay.…”

Section: References and Other Remarkssupporting

confidence: 71%

Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Luong

Vashist

2017

Biosensors and Bioelectronics

View full text Add to dashboard Cite

“…This immunosensor, which exhibited a low LOD of 0.3 pg mL À 1 , provided results in serum samples in agreement with the ELISA method. Samanman et al (2015) developed a highly-sensitive label-free electrochemical CEA immunosensor by preparing a cryogel from an AuNPs-graphene-chitosan nanocomposite (AuNPs-Gr-CS) modified on a silver deposited Au electrode. This nanocomposite combined the advantages of graphene, AuNPs (excellent biocompatibility and large surface area) and chitosan (good adhesion, non-toxicity and abundant reactive amino groups) together with the ease to prepare a cryogel (better electron transfer and larger surface area) coupled to a silver deposition, to act as a redox mediator, on an Au electrode.…”

Section: Graphene-aunps Nanohybridsmentioning

confidence: 99%

Hybrid 2D-nanomaterials-based electrochemical immunosensing strategies for clinical biomarkers determination

Campuzano

Pedrero

Nikoleli

et al. 2017

Biosensors and Bioelectronics

View full text Add to dashboard Cite

Owing to the outstanding conductivity and biocompatibility as well as numerous other fascinating properties of two-dimensional (2D)-nanomaterials, 2D-based nanohybrids have shown unparalleled superiorities in the field of electrochemical biosensors. This review highlights latest advances in electrochemical immunosensors for clinical biomarkers based on different hybrid 2D-nanomaterials. Particular attention will be given to hybrid nanostructures involving graphene and other graphene-like 2D-layered nanomaterials (GLNs). Several recent strategies for using such 2D-nanomaterial heterostructures in the development of modern immunosensors, both for tagging or modifying electrode transducers, are summarized and discussed. These hybrid nanocomposites, quite superior than their rival materials, will undoubtedly have an important impact within the near future and not only in clinical areas. Current challenges and future perspectives in this rapidly growing field are also outlined.

show abstract

“…[3][4][5][6][7] Many different electrochemical methods were used in order to fabrication of immunosensor and detection of biomarkers. [3][4][5][6][7] Many different electrochemical methods were used in order to fabrication of immunosensor and detection of biomarkers.…”

Section: Introductionmentioning

confidence: 99%

Highly-sensitive label-free immunosensor for tumor necrosis factor α based on Ag@Pt core–shell nanoparticles supported on MWCNTs as an efficient electrocatalyst nanocomposite

Mazloum-Ardakani

Hosseinzadeh

2015

RSC Adv.

View full text Add to dashboard Cite

We have developed a novel and label-free electrochemical immunosensor for detection of tumor necrosis factor-alpha (TNF-a) based on bimetallic silver@platinum (Ag@Pt) core-shell nanoparticles supported on MWCNTs and chitosan (Ag@Pt-CNTs-CS) as a desirable sensor platform. The redox reaction of catechol on the graphite screen-printed electrodes modified with Ag@Pt-CNTs-CS applied as a probe for studying the immobilization and determination processes in immunosensor, due to enhanced electrocatalytic activity of Ag@Pt-CNTs for catechol. The results showed that this nanocomposite exhibited attractive electrocatalytic activity and also yielded a large capacity for antibody loading, which improved the amount of immobilized antibody. A decrease in differential pulse voltammetry (DPV) responses was observed with increasing concentrations of TNF-a in standard and real samples due to its obstruction to the electrocatalytic oxidation of catechol by Ag@Pt-CNTs after binding to the surface of the electrode through interaction with the antibody. The detection limit for TNF-a was found to be 1.6 pg mL À1 and the detection concentrations were in the range between 6.0 and 60 pg mL À1 . The proposed electrochemical immunosensor showed high sensitivity, specificity, good reproducibility and stability as well as acceptable accuracy for TNF-a detection in human serum samples and this strategy might also be applied to detect other antigens.

show abstract

Highly-sensitive label-free electrochemical carcinoembryonic antigen immunosensor based on a novel Au nanoparticles–graphene–chitosan nanocomposite cryogel electrode

Cited by 81 publications

References 38 publications

Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Immunosensing procedures for carcinoembryonic antigen using graphene and nanocomposites

Hybrid 2D-nanomaterials-based electrochemical immunosensing strategies for clinical biomarkers determination

Highly-sensitive label-free immunosensor for tumor necrosis factor α based on Ag@Pt core–shell nanoparticles supported on MWCNTs as an efficient electrocatalyst nanocomposite

Contact Info

Product

Resources

About