Highly sensitive nested polymerase chain reaction to improve the detection of Leishmania species in clinical specimens

Tawfeeq, Hassan Mohammad; Ali, Shahnaz AbdulKader

doi:10.1007/s12639-022-01491-5

Cited by 2 publications

(2 citation statements)

References 48 publications

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“…Since its creation by Mullis in 1983, PCR has developed into a crucial diagnostic tool. Several molecular assays have been described for the detection and differentiation of parasites, including Ta e n i a species, using genomic or mitochondrial DNA: multiplex-PCR, nested PCR, quantitative real-time PCR, PCR-Restriction Fragment Length Polymorphism, a base excision sequence scanning thymine-base method (Yamasaki et al, 2002) and random amplified polymorphic DNA (RAPD) [34][35][36][37][38].…”

Section: Molecular Techniquesmentioning

confidence: 99%

Advances in the Diagnosis of Cysticercosis

Mohammad Tawfeeq

2024

Infectious Diseases

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Human cysticercosis is one of the most pathogenic and lethal diseases. It is caused by the accidental ingestion of Taenia solium eggs. All Taenia species lead to cysticercosis in animals; T. solium and Torenia asiatica are responsible for cysticercosis in pigs, while T. saginata causes bovine cysticercosis. Cysticercosis in humans is considered a neglected tropical disease. Diagnosing taeniasis—an infection with the adult parasite—poses challenges. The clinical manifestations of the disease are nonspecific, and no easy method is available to confirm the diagnosis. The diagnosis of cysticercosis is mainly based on imaging techniques, including computed tomography and magnetic resonance. These techniques are valuable and accurate but sometimes limited due to atypical images that are difficult to distinguish from neoplasms. Therefore, sensitive and specific methods, such as immunological tests and molecular methods, are essential to confirm clinical findings and differentiate cysticercosis from other diseases.

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Section: Molecular Techniquesmentioning

confidence: 99%

Advances in the Diagnosis of Cysticercosis

Mohammad Tawfeeq

2024

Infectious Diseases

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“…Interestingly, the polymerase chain reaction (PCR) test was greatly specific (100%) and sensitive (92.5%) for CL diagnosis. Tawfeeq and Ali (8) reported that microscopy, conventional PCR, and nested PCR had a sensitivity of 80.7%, 86.6%, and 100%, respectively, and all three tools had 100% specificity. We believe that in addition to confirming CL diagnosis, PCR is useful in defining the correlation between the clinical pictures of the disease and the type of Leishmania species.…”

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confidence: 99%

Is the Clinical Diagnosis of Cutaneous Leishmaniasis Justified in Endemic Regions?

Al-Mendalawi

2023

Al-Kindy Col. Med. J

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Leishmaniasis is a transmissible infection brought about by an obligatory intracellular protozoan from the genus Leishmania. It occurs worldwide in tropical and subtropical regions and can be burdensome in resource-constrained countries. The infection ranges in severity from mild cutaneous lesions to more severe and sometimes life-threatening visceral and distorting mucocutaneous sicknesses. Importantly, cutaneous leishmaniasis (CL) is prevalent in the Middle East with a pooled prevalence of 12%. It imposes a significant health and socioeconomic burden

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Highly sensitive nested polymerase chain reaction to improve the detection of Leishmania species in clinical specimens

Cited by 2 publications

References 48 publications

Advances in the Diagnosis of Cysticercosis

Advances in the Diagnosis of Cysticercosis

Is the Clinical Diagnosis of Cutaneous Leishmaniasis Justified in Endemic Regions?

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