Highly Sensitive SERS Detection of As³⁺ Ions in Aqueous Media using Glutathione Functionalized Silver Nanoparticles

Abstract

A highly sensitive surface-enhanced Raman scattering (SERS) platform for the selective trace analysis of As3+ ions was reported based on glutathione (GSH)/4-mercaptopyridine (4-MPY)-modified silver nanoparticles (AgNPs). Here, GSH conjugated on the surface of AgNPs for specifical binding with As3+ ions in aqueous solution through As–O linkage and 4-MPY was used as a Raman reporter. When As3+ ions were added to the system, the binding of As3+ with GSH resulted in the aggregation of AgNPs, and excellent Raman signal of 4-MPY reporters was obtained which can reflect the concentration of As3+ indirectly. Under optimal assay conditions, the limit of detection (LOD) was estimated to be as low as 0.76 ppb, which is lower than the WHO defined limit (10 ppb), and an excellent linear range of 4–300 ppb was obtained. The practical application had been carried out for determination of As3+ in real water samples.

DNA adsorption by magnetic iron oxide nanoparticles and its application for arsenate detection

“…6 The detection task is mainly carried out using analytical instruments such as atomic emission 7 or absorption spectroscopy, 8 ICP-MS, 9 or surface enhanced Raman scattering. 10,11 Developing cost-effective biosensors might provide a complementary solution. 12 For example, genetically engineered bacterial cells, 13 and enzyme inhibition assays were developed to detect arsenic.…”

Surface-enhanced Raman scattering microfluidic sensor

“…For this purpose, the nanoparticles such as core-shell-type nanoparticles including gold nanoshells, [99][100][101][102] nanocrescents, 103 and hollow gold nanospheres [104][105][106][107] have been synthesized, and their applicability as effective SERS probes has been tested. 77,85 The different shape nanoparticles have potential to meet the requirements of the SERS measurement. More importantly, gold nanorods (GNRs) have been introduced as powerful imaging probes for biomedical and biological applications.…”

Ultrasensitive surface-enhanced Raman scattering detection of trypsin based on anti-aggregation of 4-mercaptopyridine-functionalized silver nanoparticles: an optical sensing platform toward proteases

“…21 Up to now, various AgNPs-based SERS applications have been reported. 16,[21][22][23][24] For example, Ren et al have reported the determination of mercury ions by a direct SERS technique based on the interaction between AgNPs and mercury ions. 21 Jiang et al have developed a AgNPs aggregates-based SERS substrate for sensitive detection of polycyclic aromatic hydrocarbons.…”

“…22 Our group has developed a highly sensitive SERS strategy for detection of arsenic ions by using glutathione functionalized AgNPs. 23 In general, SERS-based assay is conducted by two modes, i.e., via controlling distance between reporter and substrate, and based on aggregation. 24 However, to the best of our knowledge, there have been no examples based on anti-aggregation of AgNPs for SERS detection of proteases.…”