Highly sensitive signal detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip: confocal surface-enhanced Raman spectroscopic study

Park, Taehan; Lee, Sangyeop; Seong, Gi Hun; Choo, Jaebum; Lee, Eun Kyu; Kim, Yang S.; Ji, Won Ho; Hwang, Seung Yong; Gweon, Dae−Gab; Lee, Sanghoon

doi:10.1039/b414457k

Cited by 135 publications

(104 citation statements)

References 46 publications

(47 reference statements)

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“…35,37 The other is called heterogeneous SERS, for which the target in solution interacts with patterned metallic structure on the substrate. 20,23,[37][38][39][40] When detecting low concentration of analytes, the heterogeneous SERS method is usually adopted owing to its better sensitivity. However, diffusion of analytes to the SERS active site is time-consuming and fabrication of substrate with uniform SERS effect is relatively complicated.…”

Section: Introductionmentioning

confidence: 99%

“…21 In recent years, surface-enhanced Raman spectroscopy (SERS) has been utilized to detect biomolecules such as cells, bacteria, viruses, and DNAs. [22][23][24][25] SERS effect was first observed on the roughened silver electrode with pyridine monolayer 26 and has been investigated for decades. [27][28][29][30][31] It originates from the surface plasmon resonance (SPR) which is the result of interaction between nano-scaled metallic structure and the excitation laser.…”

Section: Introductionmentioning

confidence: 99%

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Electrokinetic trapping and surface enhanced Raman scattering detection of biomolecules using optofluidic device integrated with a microneedles array

Deng

Juang

2013

Biomicrofluidics

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In this study, microneedles which possess sharp tips were utilized to trap and detect the biomolecules. Owing to the large curvature, the tips of the microneedles created a substantially high gradient of electric field under the non-uniform electric field which served as not only the trapping sites but also the substrate for surface enhanced Raman scattering (SERS). Separation of polystyrene microparticles with different sizes and two kinds of biomolecules (Staphylococcus aureus (S. aureus) and the red blood cells (RBCs)) were demonstrated. Moreover, in situ detection of S. aureus was performed immediately after separation was completed. The results showed that, after 15 s of sample collection, the Raman signals of S. aureus were detected and greatly enhanced through SERS effect. V C 2013 American Institute of Physics. [http://dx

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Electrokinetic trapping and surface enhanced Raman scattering detection of biomolecules using optofluidic device integrated with a microneedles array

Deng

Juang

2013

Biomicrofluidics

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“…The observed spectra were rather complex but it was speculated that deconvolution by available software (although not shown) should yield better peak shapes. Park et al [21] utilized confocal SERS to maximize the sensitivity of the detection on a PDMS microchip. They emphasized the mixing efficiency aspects for the oligonucleotides and silver nanoparticles in their alligator-tooth-like design of the chip.…”

Section: Raman Detectionmentioning

confidence: 99%

Unconventional detection methods for microfluidic devices

2006

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The direction of modern analytical techniques is to push for lower detection limits, improved selectivity and sensitivity, faster analysis time, higher throughput, and more inexpensive analysis systems with ever-decreasing sample volumes. These very ambitious goals are exacerbated by the need to reduce the overall size of the device and the instrumentation -the quest for functional micrototal analysis systems epitomizes this. Microfluidic devices fabricated in glass, and more recently, in a variety of polymers, brings us a step closer to being able to achieve these stringent goals and to realize the economical fabrication of sophisticated instrumentation. However, this places a significant burden on the detection systems associated with microchip-based analysis systems. There is a need for a universal detector that can efficiently detect sample analytes in real time and with minimal sample manipulation steps, such as lengthy labeling protocols. This review highlights the advances in uncommon or less frequently used detection methods associated with microfluidic devices. As a result, the three most common methods -LIF, electrochemical, and mass spectrometric techniques -are omitted in order to focus on the more esoteric detection methods reported in the literature over the last 2 years.

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“…The detailed fabrication process for this PDMS channel has been reported elsewhere. [12][13][14][15] …”

mentioning

confidence: 99%

“…The detailed fabrication process for this PDMS channel has been reported elsewhere. [12][13][14][15] ERβ is one of the most important breast cancer markers that are closely related with cell proliferations. 16 The anti-ERβ monoclonal antibody (McAb, mouse ascites, IgM isotype; Sigma, St. Louis, MO, USA) was labeled on QD 565 using an antibody conjugation kit (Invitrogen, Eugene, OR, USA).…”

mentioning

confidence: 99%

Open Sandwich FRET Immunoassay of Estrogen Receptor β in a PDMS Microfluidic Channel

Park

Lee

Seong

et al. 2008

Bulletin of the Korean Chemical Society

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Immunoassay is a fast and cost-effective protein detection method that can be applied to clinical diagnostics and biological research. Enzyme-linked immunosorbent assay (ELISA), surface plasmon resonance (SPR), and protein array chips are the most widely used immunoassay techniques for the detection of a target protein.1,2 In all of these methods, however, antibodies or antigens should be immobilized on a solid substrate. As a result, they require a long assay time for complete immunoreaction between antigens and antibodies, being limited by the diffusion kinetics. Moreover, they also require several cycles of consecutive binding and washing steps to separate free reagents from binding reagents. To overcome the problem of slow immunoreactions in conventional immunoassay techniques, we recently reported a fast and sensitive one-step immunoassay of estrogen receptor β (ERβ) using quantum dot (QD)-based fluorescence resonance energy transfer (FRET).3 To induce a strong FRET effect, QD was used as a fluorescence donor. FRET occurs when the electronic excitation energy of a donor chromophore is transferred to a nearby acceptor molecule via a dipole-dipole interaction between the donoracceptor pair. 4,5 Conventional fluorescence dyes, which cause an appreciable overlap between the emission band of the donor and the absorption band of the acceptor, have generally been used as a donor-acceptor set. However, QD provides significant advantages over conventional fluorescence dyes, including brighter fluorescence, resistance to photobleaching, and narrow emission bands, which makes it suitable as a sensitive fluorescence donor.6-8 As a result, QD reaches a longer distance to the acceptor than conventional fluorescence dyes because of their superior optical properties. Furthermore, the QD-based FRET immunoassay of ERβ was faster (30-40 min of immunoassay time including incubation) than conventional immunoassay techniques because it does not need any solid-phase carriers and multiple washing steps for free reagent separations.3 In our previous work, the advantages of a QD-based FRET immunoassay in an open space were fully explored. Nonetheless, a faster reaction time and a smaller sample volume are still needed to apply this QD-based FRET immunoanalysis technique to clinical diagnostics.In the present work, a lab-on-a-chip technique, combined with the QD-based FRET immunoassay method, was used to develop a more sensitive and faster immunoanalysis technique. The lab-on-a-chip technique has several advantages over conventional bench-top techniques, such as minimal sample requirement, reduced reaction time, ease of use, improved product conversion, and reduced waste generation. [9][10][11] In particular, the reaction time can be greatly reduced to less than a few minutes if a properly designed channel to obtain highly efficient mixing is adopted. Furthermore, only a small reaction volume is required in the lab-ona-chip analysis. An alligator-teeth-shaped poly(dimethylsiloxane) (PDMS) microfluidic channel was fabricated to obtain...

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Highly sensitive signal detection of duplex dye-labelled DNA oligonucleotides in a PDMS microfluidic chip: confocal surface-enhanced Raman spectroscopic study

Cited by 135 publications

References 46 publications

Electrokinetic trapping and surface enhanced Raman scattering detection of biomolecules using optofluidic device integrated with a microneedles array

Electrokinetic trapping and surface enhanced Raman scattering detection of biomolecules using optofluidic device integrated with a microneedles array

Unconventional detection methods for microfluidic devices

Open Sandwich FRET Immunoassay of Estrogen Receptor β in a PDMS Microfluidic Channel

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