Highly sensitive single photon detection system for multi-lane DNA sequencer

Abstract

A multichannel fluorescence detection system for multi-lane DNA sequencer based on single photon counting is presented. The problem of elimination of the lane cross-talk caused by both optical and electronic cross-talk between the detection lanes is discussed. A novel method for elimination of the channel cross-talk is proposed. The calibration procedure for a DNA sequencer with lane cross-talk cancellation is described. Improvement in sensitivity of a DNA sequencer setup by introducing wide collection angle optical system is discussed.

Ultra sensitive sensor with enhanced dynamic range for high speed detection of multi-color fluorescence radiation

“…During last several years our research group has developed a number of single photon sensors with increased dynamic range and has demonstrated first DNA sequencing instruments possessing an ultra high sensitivity and a large detection dynamic range (Alaverdian et al, 2002;Gavrilov et al, 2003Gavrilov et al, , 2006Dhulla et al, 2005Dhulla et al, , 2007.…”

Enhancing the Linear Dynamic Range in Multi-Channel Single Photon Detector Beyond 7OD

“…A number of strategies exist for extending the LDR of single photon detectors: utilization of neutral filters, combining several photo-sensors into one detection unit and applying fiber-optic beam splitters to sample different fractions of the incident light beam, expansion of the LDR of the photon detector by post-processing of the detected signal [2], and combination of photon counting at low-light levels with analog to digital conversion at higher illumination levels [3], [4]. …”