Highly specific and sensitive anti-Strongyloides venezuelensis IgY antibodies applied to the human strongyloidiasis immunodiagnosis

Faria, Lucas Silva de; Souza, Dayane Lorena Naves de; Ribeiro, Raphaella Paula; Sousa, José Eduardo Neto de; Borges, Isabela Pacheco; Ávila, Veridiana Melo Rodrigues; Ferreira-Júnior, A.; Goulart, Luíz Ricardo; Costa-Cruz, Júlia Maria

doi:10.1016/j.parint.2019.101933

Cited by 14 publications

(18 citation statements)

References 49 publications

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“…Given the feasibility of using congeneric species to diagnose patients with parasites that share epitopes, such as Taenia solium and Taenia saginata (Oliveira et al ., 2007) and Strongyloides stercoralis and S. venezuelensis (Feliciano et al, 2010; Faria et al ., 2019), we evaluated the use of anti- A. ceylanicum IgY to detect hookworm infections caused by A. duodenale or N. americanus in Brazil, as A. ceylanicum is closely related to these species and therefore shares various antigenic determinants. In addition, unlike the major hookworm species, A. ceylanicum can infect dogs and hamsters.…”

Section: Discussionmentioning

confidence: 99%

“…IgY has been successfully used in different immunoassay formats, mainly ELISA and lateral flow immunoassays, for the detection of veterinary drugs, herbicides, mycotoxins, drugs of abuse, proteins and bacteria (Zhang et al ., 2015) for both diagnostic (Ferreira Júnior et al ., 2012; Faria et al ., 2019) and immunotherapeutic (Thirumalai et al ., 2019) purposes.…”

Section: Discussionmentioning

confidence: 99%

“…Data were expressed as the ELISA index (EI) as follows: EI = OD/cut-off of the negative controls (control group − group immunized with PBS) + three standard deviations. To establish the cut-off values for the control group, the OD values from the first 6 weeks were used (Faria et al ., 2019; Lopes et al ., 2019).…”

Section: Methodsmentioning

confidence: 99%

“…Due to the advantages mentioned above, polyclonal IgY antibodies have been widely used in applications related to parasitic diseases such as toxoplasmosis (Ferreira Júnior et al ., 2012), schistosomiasis (Lei et al ., 2012), trichinellosis (Liu et al ., 2013), strongyloidiasis (Faria et al ., 2019) and ascariasis (Lopes et al ., 2019). In view of this, we developed the first sensitive, specific and noninvasive IgY sandwich-based ELISA to detect immune complexes (ICs) in serum, indicating active hookworm infection.…”

Section: Introductionmentioning

confidence: 99%

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Use of polyclonal IgY antibodies to detect serum immune complexes in patients with active hookworm infection

Souza¹,

Faria²,

Sousa³

et al. 2020

Parasitology

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Definitive diagnosis of hookworm infection is usually based on the microscopic detection of eggs in a stool sample; however, several cases display a low or irregular egg output. Serodiagnosis can be a useful tool to identify these cases, but conventional tests do not differentiate past from active infections. The aim of this study was to obtain and apply egg yolk polyclonal immunoglobulin (IgY) antibodies to detect immune complexes (ICs) in serum samples from patients infected with hookworm. Hens were immunized with Ancylostoma ceylanicum saline extract, their eggs were collected and then IgY antibodies were extracted and purified. Antibody purity was tested by 12% sodium dodecyl sulphate polyacrylamide gel electrophoresis and specificity was assessed by immunoblotting and immunofluorescence. IgY production was evaluated by kinetics enzyme-linked immunosorbent assay (ELISA). Sandwich ELISA tested the ability of IgY to detect ICs in serum samples, from which diagnostic parameters were calculated. Antibody responses increased steadily from day 7 to 42. In the immunoblotting assay, IgY recognized two protein complexes. The immunofluorescence assay showed no staining in control samples. The sandwich ELISA presented a very high diagnostic value, with a sensitivity of 90% and a specificity of 86.7%. Our pioneer strategy highlights the potential use of egg yolk IgY as a diagnostic test to detect active hookworm infection.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Use of polyclonal IgY antibodies to detect serum immune complexes in patients with active hookworm infection

Souza¹,

Faria²,

Sousa³

et al. 2020

Parasitology

View full text Add to dashboard Cite

show abstract

“…Nevertheless, detection of circulating immune complexes (CIC) can be used for NCC diagnosis, since it has been successfully applied in helminth diagnosis approaches (Faria et al ., 2019; Lopes et al ., 2019). This method provides clarity to understand changes in the organism before and after disease treatment, since CIC can be present in plasma, and also be found attached to lymphocytes.…”

Section: Introductionmentioning

confidence: 99%

IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis usingTaenia crassicepshydrophobic antigens

Carrara¹,

Silva²,

Faria³

et al. 2019

Parasitology

Self Cite

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Human neurocysticercosis (NCC) is a worldwide neglected disease caused by Taenia solium metacestode and responsible for various complications and neurological disorders. This study aimed to evaluate the use of specific immunoglobulin Y (IgY) produced by laying hens immunized with a hydrophobic fraction of Taenia crassiceps metacestodes (hFTc) in NCC diagnosis. Egg yolk IgY antibodies were fractionated, purified and characterized. Enzyme-linked immunosorbent assay (ELISA) was carried out to evaluate the production kinetics and avidity maturation of anti-hFTc IgY antibodies throughout the IgY obtention process. Antigen recognition tests were carried out by Western blotting and immunofluorescence antibody test using purified and specific anti-hFTc IgY antibodies for detection of parasitic antigens of T. crassiceps and T. solium metacestodes. Sandwich ELISA was performed to detect circulating immune complexes formed by IgG and parasitic antigens in human sera. The results showed high diagnostic values (93.2% sensitivity and 94.3% specificity) for immune complexes detection in human sera with confirmed NCC. In conclusion, specific IgY antibodies produced from immunized hens with hFTc antigens were efficient to detect T. solium immune complexes in human sera, being an innovative and potential tool for NCC immunodiagnosis.

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Immunization of Hens

Júnior

et al. 2021

IgY-Technology: Production and Application of Egg Yolk Antibodies

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Highly specific and sensitive anti-Strongyloides venezuelensis IgY antibodies applied to the human strongyloidiasis immunodiagnosis

Cited by 14 publications

References 49 publications

Use of polyclonal IgY antibodies to detect serum immune complexes in patients with active hookworm infection

Use of polyclonal IgY antibodies to detect serum immune complexes in patients with active hookworm infection

IgY antibody and human neurocysticercosis: a novel approach on immunodiagnosis usingTaenia crassicepshydrophobic antigens

Immunization of Hens

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