1993
DOI: 10.1097/00002030-199303000-00005
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Highly specific V3 peptide enzyme immunoassay for seretyping HIV-1 specimens from Thailand

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Cited by 121 publications
(52 citation statements)
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“…Lymphocyte immunophenotyping was done on fresh EDTAanticoagulated venous samples following the first seropositive visit with the FACScan flow cytometer (Becton Dickinson Immunocytometry Systems, San Jose, Calif.) using a standard six-tube, two-color monoclonal antibody panel (Becton Dickinson). A V3 peptide-based EIA was used to measure serum antibody reactivities to HIV-1 CRF01_AE and subtype B-specific V3 peptides (26,38).…”
Section: Methodsmentioning
confidence: 99%
“…Lymphocyte immunophenotyping was done on fresh EDTAanticoagulated venous samples following the first seropositive visit with the FACScan flow cytometer (Becton Dickinson Immunocytometry Systems, San Jose, Calif.) using a standard six-tube, two-color monoclonal antibody panel (Becton Dickinson). A V3 peptide-based EIA was used to measure serum antibody reactivities to HIV-1 CRF01_AE and subtype B-specific V3 peptides (26,38).…”
Section: Methodsmentioning
confidence: 99%
“…Plasma HIV RNA level determination was done by the NU-CLISENS method with a subset of four cohorts (n ϭ 124). Viral subtyping was performed by the Department of Microbiology, Faculty of Medicine, Siriraj Hospital, Bangkok, Thailand, by a peptide ELISA (30) and heteroduplex mobility assay (10). The 14 amino acids specific for subtype E (env subtype E; T S I T I G P G Q V F Y R T) and subtype B (env subtype B; K S I H L G P G Q A W Y T T) were used with the ELISA for this study.…”
Section: Methodsmentioning
confidence: 99%
“…Endpoint titers were determined using twofold dilutions. Antigen preparation, plate coating, and ELISA methods were as described previously (22). The assay cutoff was the mean corrected optical density at 450 nm (OD 450 ) of five pools of negative control sera plus 0.150 OD units.…”
Section: Methodsmentioning
confidence: 99%