Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging

Tang, Liangfeng; Yu, Guiping; Tan, Lishan; Li, Min; Deng, Xiulong; Liu, Jianyu; Li, Aiqing; Lai, Xuandi; Hu, Jianqiang

doi:10.1038/s41598-017-08903-0

Cited by 10 publications

(8 citation statements)

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“…Among various noble metal nanomaterials, gold nanomaterials are most intensely investigated as imaging contrast agents and nanocarriers . As a result, the integration of aptamer with gold nanomaterials, such as Au nanorods (AuNRs), Au nanoparticles, Au nanocages, Au nanostars (AuNSs), hollow gold nanospheres (HAuNS), and Au nanoclusters (Au NCs), has been widely employed in in vivo applications in diverse areas, such as intracellular molecular imaging, cancer cell and tumor imaging, targeted delivery of therapeutic cargos (e.g., anticancer drug, protein, and gene), and cancer therapy . In one example, Huang et al demonstrated the use of AuNRs as an efficient and robust multivalent platform for molecular assembly of aptamers for targeted cell imaging .…”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

“…These nanoconjugates showed promising application for T1 MRI of transplanted human brain neural stem cells in vivo. Afterward, Tang et al constructed highly stabilized core satellite Au nanoassemblies (CSAuNAs) by a hierarchical DNA‐directed self‐assembly strategy, which can be selectively accumulated in the kidneys with satisfactory renal retention capability. Similarly, Si et al developed versatile drug‐loaded nanoprobes based on MUC1 aptamer‐integrated AuNPs, and further engineered telomerase‐recognition sequence to the nanoprobes, enabling controllable drug release by intracellular telomerase …”

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

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Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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equally exciting and perhaps more challenging field of application is toward their in vivo applications, including living animals and human bodies, in diverse areas, [2] such as sensing, drug delivery, medical imaging, and cancer therapy. However, most of these nanomaterials lack selectivity toward targets of interests. Therefore, to employ these nanomaterials for a wider range of in vivo applications, various molecular engineering approaches have been employed to obtained nanomaterials functionalized with biomolecules to enable selective targeting. [3][4][5] Among these biomolecules, functional nucleic acids, or FNAs, have shown the most promise.FNAs are DNA or RNA molecules that can interact with or bind to a specific analyte, resulting in a conformational change and/or a catalytic reaction. [6] As their names suggested, ribozymes and deoxyribozymes (called DNAzyme hereafter) can act as enzymes in the presence of cofactors to catalyze various reactions, including cleavage and ligation of RNA/DNA, and phosphorylation and peroxidation of DNA. On the other hand, riboswitches and DNA aptamers are antibody-like receptors that can bind target molecules with high affinity and selectivity. Furthermore, the binding abilities of riboswitches and aptamers can be combined with the enzymatic function of ribozymes or DNAzymes to form aptazymes so that the binding of targets can inhibit or enhance the catalytic activities. These nucleic acids, including DNAzymes, aptamers, and aptazymes, are collectively known as FNAs to emphasize their functions beyond the traditional genetic storage and transformations roles for DNA and RNA. Unlike DNA and RNA inside biological systems, FNAs are isolated via a combinatorial technique known as in vitro selection, or a process also known as systematic evolution of ligands by exponential enrichment (SELEX). The discovery of new functions of nucleic acids has not only resulted in major advances in the fields of molecular biology and biochemistry, but also revolutionized sensors designs for both in vitro and in vivo applications. [7,8] Over the past decade, numerous FNA-based nanomaterials have been developed. [9][10][11][12] Among these FNA-based nanosystems, the functions of FNAs can be categorized into two types: diagnostic function and therapeutic function. For the diagnostic function, the FNAs serve either as the biorecognition ligands for direct sensing and imaging of the Recent advances in nanotechnology and engineering have generated many nanomaterials with unique physical and chemical properties. Over the past decade, numerous nanomaterials are introduced into many research areas, such as sensors for environmental monitoring, food safety, point-ofcare diagnostics, and as transducers for solar energy transfer. Meanwhile, functional nucleic acids (FNAs), including nucleic acid enzymes, aptamers, and aptazymes, have attracted major attention from the biomedical community due to their unique target recognition and catalytic properties. Benefiting from the recent progress of molecular engine...

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Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Section: Molecular Engineering Of Aptamer‐based Nanomaterials Toward mentioning

confidence: 99%

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Zhang

Lan

2019

Adv Healthcare Materials

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“…While smaller inorganic nanoparticles such as gold, silver, or semiconductor NPs as satellites are explored. The nano spacer linkers that are commonly employed include (1) protein based assembly 41 , (2) polymeric based assembly [42][43][44] , (3) DNA -assisted assembly 36, [45][46][47][48][49] , and (4) molecular linkers that directly bridge the plasmonic units using either covalent or electrostatic interactions of the functional groups 37,50-52 . For example, Chen et al demonstrated the synthesis of streptavidin-coated polymeric bead core -Au satellite assembly via DNA linkers 53 .…”

Section: Introductionmentioning

confidence: 99%

“…However, the use of DNA is often laborious and expensive. Typically, DNA mediated core-satellite assemblies require at least ~17 hours to finish the reaction with many subsequent centrifugation steps 36, [45][46][47][48][49] . Moderate enhancing capabilities are observed within the DNA mediated assembly due to the intrinsic DNA length required to ensure stable hybridization precluding tunability of the interparticle gap distances between the core-satellite nanostructures 53,54 .…”

Section: Introductionmentioning

confidence: 99%

Synthesis of SERS-active core–satellite nanoparticles using heterobifunctional PEG linkers

Juan

Chavva

et al. 2022

Nanoscale Adv.

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“…DNA-Au nanoparticle (DNA-AuNP) conjugates, one of the most versatile hybrid bionanomaterials, provide excellent platforms for chemical and biological applications, such as biosensing, cellular imaging, drug delivery, and therapeutics. − In general, the nanoconjugates typically turn to physical or chemical interaction to self-assemble oligonucleotides on the AuNPs surfaces. For the first type, DNA is adsorbed onto the surfaces of AuNPs with a random coil structure via noncovalent interaction. , The preparation process of such DNA-AuNPs is simple and time-saving; however, their applicability is greatly confined since the physically based nanoconjugates merely tolerate the concentration of 20 mM NaCl in the solution according to the previous report, and thereby they are incapable of stably existing in the complicated biological matrix.…”

mentioning

confidence: 99%

Robust and Universal SERS Sensing Platform for Multiplexed Detection of Alzheimer’s Disease Core Biomarkers Using PAapt-AuNPs Conjugates

et al. 2019

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Multiplexed detection of Alzheimer's disease (AD) core biomarkers is of great significance to early diagnosis and personalized treatment of AD patients. Herein, we construct a robust and convenient surface-enhanced Raman scattering (SERS) biosensing platform for simultaneous detection of Aβ(1−42) oligomers and Tau protein using different Raman dye-coded polyA aptamer-AuNPs (PAapt-AuNPs) conjugates. This strategy relies on the specific protein−aptamer binding-mediated aggregation of AuNPs and the concomitant plasmonic coupling effect that allow us to "turn on" SERS detection of protein biomarkers. To the best of our knowledge, this is the first work in which PAapt-AuNPs conjugates are used for probing protein biomarkers, which may be enlightening for the exploitation of more extensive biological applications of aptamer-AuNPs conjugates. The results reveal that the present strategy displays excellent analytical performance. Moreover, the applicability of this strategy is demonstrated in the artificial cerebrospinal fluid (CSF) samples with satisfactory results. Except for the prominent sensitivity and practicality, our strategy offers additional advantages. The preparation of nanoconjugates is handy and easily repeated, and the synthesis cost is greatly reduced because it dispenses with the complicated labeling process. Moreover, the assay can be accomplished in 15 min, allowing rapid detection of protein biomarkers. Furthermore, simultaneous detection of Tau protein and Aβ(1−42) oligomers is realized by employing different Raman dye-coded nanoconjugates, which is valuable for accurately predicting and diagnosing AD disease. Thus, our PAapt-AuNPs conjugate-based multiplexed SERS strategy indeed creates a useful and universal platform for detecting multiple protein biomarkers and related clinical diagnosis.

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Highly Stabilized Core-Satellite Gold Nanoassemblies in Vivo: DNA-Directed Self-Assembly, PEG Modification and Cell Imaging

Cited by 10 publications

References 48 publications

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Molecular Engineering of Functional Nucleic Acid Nanomaterials toward In Vivo Applications

Synthesis of SERS-active core–satellite nanoparticles using heterobifunctional PEG linkers

Robust and Universal SERS Sensing Platform for Multiplexed Detection of Alzheimer’s Disease Core Biomarkers Using PAapt-AuNPs Conjugates

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