Highly Stable Near-Infrared Fluorescent Organic Nanoparticles with a Large Stokes Shift for Noninvasive Long-Term Cellular Imaging

Zhang, Jinfeng; Chen, Rui; Zhu, Ze‐Lin; Adachi, Chihaya; Zhang, Xiaohong; Lee, Chun‐Sing

doi:10.1021/acsami.5b08539

Cited by 131 publications

(103 citation statements)

References 64 publications

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“…Fluorescence 3D construction has been performed using vector analysis with respect to the tumor and detector coordinates, which is utilized for the calculation of tumor voxel intensities [83]. A recent study by Michele et al has shown the 3D modelling of tumor spheroids for in vitro analysis [84].…”

Section: 3d Tomographymentioning

confidence: 99%

Near Infrared Fluorescence Imaging in Nano-Therapeutics and Photo-Thermal Evaluation

Vats

Mishra

Baghini

et al. 2017

IJMS

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The unresolved and paramount challenge in bio-imaging and targeted therapy is to clearly define and demarcate the physical margins of tumor tissue. The ability to outline the healthy vital tissues to be carefully navigated with transection while an intraoperative surgery procedure is performed sets up a necessary and under-researched goal. To achieve the aforementioned objectives, there is a need to optimize design considerations in order to not only obtain an effective imaging agent but to also achieve attributes like favorable water solubility, biocompatibility, high molecular brightness, and a tissue specific targeting approach. The emergence of near infra-red fluorescence (NIRF) light for tissue scale imaging owes to the provision of highly specific images of the target organ. The special characteristics of near infra-red window such as minimal auto-fluorescence, low light scattering, and absorption of biomolecules in tissue converge to form an attractive modality for cancer imaging. Imparting molecular fluorescence as an exogenous contrast agent is the most beneficial attribute of NIRF light as a clinical imaging technology. Additionally, many such agents also display therapeutic potentials as photo-thermal agents, thus meeting the dual purpose of imaging and therapy. Here, we primarily discuss molecular imaging and therapeutic potentials of two such classes of materials, i.e., inorganic NIR dyes and metallic gold nanoparticle based materials.

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Section: 3d Tomographymentioning

confidence: 99%

Near Infrared Fluorescence Imaging in Nano-Therapeutics and Photo-Thermal Evaluation

Vats

Mishra

Baghini

et al. 2017

IJMS

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“…Small organic molecules can be highly fluorescent, although they may suffer poor solubility in aqueous media (particularly so for red and infrared dyes), limiting their use for bioapplications . Loading an organic dye into a nonfluorescent nanocarrier, arranged as fluorescent organic nanoparticle (FON), offers an interesting strategy to bring organic dyes in aqueous media, since the obtained FONs have in general good brightness, photostability, and biocompatibility . These nanostructures also have well‐known physicochemical properties and can be loaded or grafted with bioactive compounds and/or targeting agents, to produce multifunctional nanoparticles for theranostic applications …”

mentioning

confidence: 99%

Nanostructuring Lipophilic Dyes in Water Using Stable Vesicles, Quatsomes, as Scaffolds and Their Use as Probes for Bioimaging

Ardizzone

Illa-Tuset

Faraudo

et al. 2018

Small

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A new kind of fluorescent organic nanoparticles (FONs) is obtained using quatsomes (QSs), a family of nanovesicles proposed as scaffolds for the nanostructuration of commercial lipophilic carbocyanines (1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indocarbocyanine perchlorate (DiI), 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indodicarbocyanine perchlorate (DiD), and 1,1'-dioctadecyl-3,3,3',3'-tetramethyl-indotricarbocyanine iodide (DiR)) in aqueous media. The obtained FONs, prepared by a CO -based technology, show excellent colloidal- and photostability, outperforming other nanoformulations of the dyes, and improve the optical properties of the fluorophores in water. Molecular dynamics simulations provide an atomistic picture of the disposition of the dyes within the membrane. The potential of QSs for biological imaging is demonstrated by performing superresolution microscopy of the DiI-loaded vesicles in vitro and in cells. Therefore, fluorescent QSs constitute an appealing nanomaterial for bioimaging applications.

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“…This system is a good candidate for performing fluorescence imaging of cancer over an extended period of time. 168 …”

Section: Polymer-core and Fluorophore Nanoparticlesmentioning

confidence: 99%

Mini-review: fluorescence imaging in cancer cells using dye-doped nanoparticles

2016

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Fluorescence imaging has gained increased attention over the past two decades as a viable means to detect a variety of cancers. Fluorescence imaging has the potential to provide physicians with high resolution images with enhanced contrast, which will allow them to be able to better diagnose and treat patients with cancer. Early detection and treatment are key to erradicating cancer in a patient, and fluorescence imaging has the ability to identify non-advanced, even pre-cancerous, tumors where imaging based on white light or radiation overlooked them. Several fluorescent dyes have been identified as possible fluorophores for enhanced fluorescence imaging, such as cyanine, squaraine, porphyrin, phthalocyanine, and borondipyrromethane dyes. These dyes have high fluorescence quantum yields, which provides a high target to background ratio; however, these dyes are often plagued by low water solubility. This low solubility can be ameliorated by conjugating or covalently attaching these dyes to polymeric crosslinked micelles, polymersomes, or polymer-core nanoparticles. These particle & dye systems then can become platforms on which secondary components can be attached to enhance the systems functionality. For example, dyes attached to these nanocarriers can target tumors through passive targeting; however, active targeting can be achieved by further modifying these nanocarriers with ligands that have a binding affinity for receptors overexpressed in tumor cells, cell surface receptors located on the tumor cell membrane, or endothelium. Fluorescence activation of the probes is another promising technology for the early detection of cancer. Activation requires that there be a change in fluorescence, whether it be an emission wavelength change or a fluorescence "on/off" signal when in the presence of some external stimuli. Activation increase the target to background ratio and enhances the contrast of the obtained image. This review serves to highlight the recent developments of (1) improved fluorescent dyes for the detection of cancer, with a specific focus on dyes that are being coupled to nanocarriers; (2) dye & nanocarrier systems that target, both actively and passively, tumors, and (3) fluorescence activation of these fluorophore systems for better image quality.

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Highly Stable Near-Infrared Fluorescent Organic Nanoparticles with a Large Stokes Shift for Noninvasive Long-Term Cellular Imaging

Cited by 131 publications

References 64 publications

Near Infrared Fluorescence Imaging in Nano-Therapeutics and Photo-Thermal Evaluation

Near Infrared Fluorescence Imaging in Nano-Therapeutics and Photo-Thermal Evaluation

Nanostructuring Lipophilic Dyes in Water Using Stable Vesicles, Quatsomes, as Scaffolds and Their Use as Probes for Bioimaging

Mini-review: fluorescence imaging in cancer cells using dye-doped nanoparticles

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