Highly stable selenium nanoparticles: Assembly and stabilization via flagellin FliC and porin OmpF in Rahnella aquatilis HX2

Li, Kui; Xu, Qiaolin; Gao, Shanshan; Zhang, Sasa; Ma, Yuhui; Zhao, Guishen; Guo, Yanbin

doi:10.1016/j.jhazmat.2021.125545

Cited by 27 publications

(21 citation statements)

References 66 publications

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“…Many researchers have carried out relevant studies, and the results show that Saccharomyces boulardii , Bacillus subtilis , Escherichia coli , green algae and other organisms can reduce Se cations to Se 0 under aerobic or anaerobic conditions without additional chemical reagents ( Fesharaki et al, 2010 ; Song et al, 2017b ; Asghari-Paskiabi et al, 2020 ; Li et al, 2021 ). In addition, BioSeNPs are able to combine with SeCys, SeMet, selenoprotein and other compounds and participate in many biological processes of organisms ( Loeschner et al, 2014 ; Hadrup et al, 2016 ; Hadrup et al, 2019 ).…”

Section: The Green Synthesis Of Biosenpsmentioning

confidence: 99%

Bioconversion of inorganic selenium to less toxic selenium forms by microbes: A review

Nie

Yang²,

He³

et al. 2023

Front. Bioeng. Biotechnol.

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In recent years, microbial conversion of inorganic selenium into an efficient and low-toxic form of selenium has attracted much attention. With the improvement of scientific awareness and the continuous progress of nanotechnology, selenium nanoparticles can not only play the unique functions of organic selenium and inorganic selenium but also have higher safety, absorption and biological activity than other selenium forms. Therefore, the focus of attention has gradually shifted beyond the level of selenium enrichment in yeast to the combination of biosynthetic selenium nanoparticles (BioSeNPs). This paper primarily reviews inorganic selenium and its conversion to less toxic organic selenium and BioSeNPs by microbes. The synthesis method and potential mechanism of organic selenium and BioSeNPs are also introduced, which provide a basis for the production of specific forms of selenium. The methods to characterize selenium in different forms are discussed to understand the morphology, size and other characteristics of selenium. In general, to obtain safer and higher selenium content products, it is necessary to develop yeast resources with higher selenium conversion and accumulation.

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Section: The Green Synthesis Of Biosenpsmentioning

confidence: 99%

Bioconversion of inorganic selenium to less toxic selenium forms by microbes: A review

Nie

Yang²,

He³

et al. 2023

Front. Bioeng. Biotechnol.

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“…8,9 To defend against excessive toxic chemicals and eliminate high surface energy, defensive proteins, such as bacterial elongation flagellin (FliC), can assemble SeNPs by coating the surface of the nanoparticles and promoting stability. 7 These coating proteins, named protein coronas, are key components that direct biological activity; can change the size, morphology, stability, surface charge, and resistance to aggregation of nanoparticles; reduce the toxicity of nanoparticles; and provide a practicable drug delivery system for biomedical applications. 10,11 In addition, the composition of the protein corona depends on the type of biological source and nanoparticle and is believed to be biologically related to the nanoparticles, which affects circulation time, stability, and cellular interaction/uptake, thus having a significant impact on their function.…”

Section: Introductionmentioning

confidence: 99%

Superoxide Dismutase Catalyzed Size-Adjustable Selenium Nanoparticles in Saccharomyces boulardii

Yang,

Song,

Gao

et al. 2024

J. Agric. Food Chem.

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Selenium nanoparticles (SeNPs) are important and safe food and feed additives that can be used for dietary supplementation. In this study, a mutagenic strain of Saccharomyces boulardii was employed to obtain biologically synthesized SeNPs (BioSeNPs) with the desired particle size by controlling the dosage and duration of sodium selenite addition, and the average particle size achieved was 55.8 nm with protease A encapsulation. Transcriptomic analysis revealed that increased expression of superoxide dismutase 1 (SOD1) in the mutant strain effectively promoted the synthesis of BioSeNPs and the formation of smaller nanoparticles. Under sodium selenite stress, the mutant strain exhibited significantly increased expression of glutathione peroxidase 2 (GPx2), which was significantly greater in the mutant strain than in the wild type, facilitating the synthesis of glutathione selenol and providing abundant substrates for the production of BioSeNPs. Furthermore, based on the experimental results and transcriptomic analysis of relevant genes such as sod1, gpx2, the thioredoxin reductase 1 gene (trr1) and the thioredoxin reductase 2 gene (trr2), a yeast model for the size-controlled synthesis of BioSeNPs was constructed. This study provides an important theoretical and practical foundation for the green synthesis of controllable-sized BioSeNPs or other metal nanoparticles with potential applications in the fields of food, feed, and biomedicine.

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“…El-Saadony et al 15 biosynthesized SeNPs with an average particle size of 56.91 ± 1.8 nm from lactic acid bacteria (LAB) isolated from human milk. Li et al 16 used the plant inter-rhizosphere-promoting bacterium Rahnella aquatilis HX2 to reduce selenite into BioSeNPs, and found that both FliC and OmpF proteins could control the assembly and stability of BioSeNPs. Sun et al 17 synthesized spherical SeNPs with a diameter of approximately 48 nm from selenium-rich strain 2322 Rhodobacter erythropolis through fermentation.…”

Section: Introductionmentioning

confidence: 99%

Development and application of a new biological nano-selenium fermentation broth based on Bacillus subtilis SE201412

Huang

Wen

et al. 2023

Sci Rep

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In order to improve the functionality and additional value of agricultural products, this study developing nano-selenium fermentation broth and established a new application strategy of bio-nano-selenium by screening and identifying selenium-rich microorganisms. We isolated a new strain from tobacco waste and named it Bacillus subtilis SE201412 (GenBank accession no. OP854680), which could aerobically grow under the condition of 66,000 mg L−1 selenite concentration, and could convert 99.19% of selenite into biological nano-selenium (BioSeNPs) within 18 h. Using strain SE201412, we industrially produced the different concentrations of fermentation broth containing 5000–3000 mg L−1 pure selenium for commercial use. The synthesized selenium nanoparticles (SeNPs) were characterized by scanning electron microscopy (SEM), transmission electron microscopy (TEM), and nanoparticle tracking analysis (NTA). TEM and SEM results showed that SeNPs were distributed outside cells. NTA assay of fermentation broth indicated that the nanoparticles were spherical with an average particle size of 126 ± 0.5 nm. Toxicity test revealed that the median lethal dose (LD50) of the fermentation broth to mice was 2710 mg kg−1, indicating its low toxicity and high safety. In addition, we applied BioSeNP fermentation broth to rice and wheat through field experiments. The results showed that the application of fermentation broth significantly increased the total selenium content and organic selenium percentage in rice and wheat grains. Our findings provide valuable reference for the development of BioSeNPs with extensive application prospects.

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Highly stable selenium nanoparticles: Assembly and stabilization via flagellin FliC and porin OmpF in Rahnella aquatilis HX2

Cited by 27 publications

References 66 publications

Bioconversion of inorganic selenium to less toxic selenium forms by microbes: A review

Bioconversion of inorganic selenium to less toxic selenium forms by microbes: A review

Superoxide Dismutase Catalyzed Size-Adjustable Selenium Nanoparticles in Saccharomyces boulardii

Development and application of a new biological nano-selenium fermentation broth based on Bacillus subtilis SE201412

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