Highly Synergistic Effects of Melittin with Conventional Antibiotics Against Multidrug-Resistant Isolates of <i>Acinetobacter baumannii</i> and <i>Pseudomonas aeruginosa</i>

Akbari, Reza; Hakemi-Vala, Mojdeh; Pashaie, Fatemeh; Bevalian, Parvaneh; Hashemi, Ali; Bagheri, Kamran Pooshang

doi:10.1089/mdr.2018.0016

Cited by 60 publications

(57 citation statements)

References 33 publications

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“…The above advantages and rich resource content of insect AMPs make them excellent templates for the development of new antimicrobial drugs. Indeed, some synergistic effects of AMPs with conventional antibiotics are observed against bacteria [ 141 ]. However, quantitative methods are rarely used to test this synergistic profile.…”

Section: Discussionmentioning

confidence: 99%

“…Very recently, Akbari et al [ 141 ] showed that there are highly synergistic effects of melittin with conventional antibiotics against MDR isolates of Acinetobacter baumannii and P. aeruginosa . In their study, the geometric means of MIC for melittin and doripenem after combination were reduced to 61.5- and 51.5-fold, respectively, against A. baumannii isolates.…”

Section: Insect Antimicrobial Peptidesmentioning

confidence: 99%

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Insect Antimicrobial Peptides, a Mini Review

Patočka

Kuča

2018

Toxins

384

271

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Antimicrobial peptides (AMPs) are crucial effectors of the innate immune system. They provide the first line of defense against a variety of pathogens. AMPs display synergistic effects with conventional antibiotics, and thus present the potential for combined therapies. Insects are extremely resistant to bacterial infections. Insect AMPs are cationic and comprise less than 100 amino acids. These insect peptides exhibit an antimicrobial effect by disrupting the microbial membrane and do not easily allow microbes to develop drug resistance. Currently, membrane mechanisms underlying the antimicrobial effects of AMPs are proposed by different modes: the barrel-stave mode, toroidal-pore, carpet, and disordered toroidal-pore are the typical modes. Positive charge quantity, hydrophobic property and the secondary structure of the peptide are important for the antibacterial activity of AMPs. At present, several structural families of AMPs from insects are known (defensins, cecropins, drosocins, attacins, diptericins, ponericins, metchnikowins, and melittin), but new AMPs are frequently discovered. We reviewed the biological effects of the major insect AMPs. This review will provide further information that facilitates the study of insect AMPs and shed some light on novel microbicides.

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Section: Discussionmentioning

confidence: 99%

Section: Insect Antimicrobial Peptidesmentioning

confidence: 99%

Insect Antimicrobial Peptides, a Mini Review

Patočka

Kuča

2018

Toxins

384

271

View full text Add to dashboard Cite

show abstract

“…Although the mechanism of action of the different AMPs has not yet been fully elucidated, it appears that AMPs, unlike antibiotics, have more difficulty in causing a microbial resistance, and most of them do not destroy normal cells of higher animals 46 . Recently, it has been demonstrated that the clavaspirin peptide from tunicate Styela clava exhibits the ability to kill drug-resistant pathogens, such as S. aureus, without a detectable resistance 47 . Moreover, it was demonstrated that two proline rich peptides (Lser-PRP2 and Lser-PRP3) do not interfere with protein synthesis but both were able to bind the bacterial chaperone DnaK and are therefore able to inhibit protein folding 48 .…”

Section: Discussionmentioning

confidence: 99%

A bioinformatic study of antimicrobial peptides identified in the Black Soldier Fly (BSF) Hermetia illucens (Diptera: Stratiomyidae)

Moretta

Salvia

Scieuzo

et al. 2020

Sci Rep

119

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Antimicrobial peptides (AMPs) play a key role in the innate immunity, the first line of defense against bacteria, fungi, and viruses. AMPs are small molecules, ranging from 10 to 100 amino acid residues produced by all living organisms. Because of their wide biodiversity, insects are among the richest and most innovative sources for AMPs. In particular, the insect Hermetia illucens (Diptera: Stratiomyidae) shows an extraordinary ability to live in hostile environments, as it feeds on decaying substrates, which are rich in microbial colonies, and is one of the most promising sources for AMPs. The larvae and the combined adult male and female H. illucens transcriptomes were examined, and all the sequences, putatively encoding AMPs, were analysed with different machine learning-algorithms, such as the Support Vector Machine, the Discriminant Analysis, the Artificial Neural Network, and the Random Forest available on the CAMP database, in order to predict their antimicrobial activity. Moreover, the iACP tool, the AVPpred, and the Antifp servers were used to predict the anticancer, the antiviral, and the antifungal activities, respectively. The related physicochemical properties were evaluated with the Antimicrobial Peptide Database Calculator and Predictor. These analyses allowed to identify 57 putatively active peptides suitable for subsequent experimental validation studies.

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“…Recent studies have demonstrated the synergistic activity of antibiotics combined with AMPs. Akbari et al (2019) reported the synergism and other drug interactions between melittin, a cationic amphipathic peptide, and antibiotics such as doripenem, doxycycline, colistin, and ceftazidime, against MDR isolates of A. baumannii and P. aeruginosa. Likewise, combinatorial treatments of conventional antibiotics with new synthetic peptides inspired by human cationic peptides LL-37 and thrombocidin-1 (TC-1) have shown synergistic activity against S. aureus (antibacterial and anti-biofilm activity; Koppen et al, 2019).…”

Section: Antimicrobial Peptide-based Combinatorial Treatmentsmentioning

confidence: 99%