Highly Transferable pAQU-Related Plasmids Encoding Multidrug Resistance Are Widespread in the Human and Fish Pathogen Photobacterium damselae subsp. damselae in Aquaculture Areas in the Black Sea

Vences, Ana; Abushattal, Saqr; Matanza, Xosé M.; Dubert, Javier; Uzun, Ecren; Öğüt, Hamdi; Osorio, Carlos R.

doi:10.1007/s00248-020-01519-4

Cited by 4 publications

(4 citation statements)

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“…Besides that, the horizontal transfer of antibiotic‐resistant pathogens from wild‐caught fish to cage‐cultured fish and the development of AMR bacteria in cage‐cultured fish and their pathogenic potential affects the seafood safety and economy of the mariculture industry. Various studies have reported the occurrence of antibiotic‐resistant bacteria in the marine ecosystem (Chiu et al, 2012; Jun et al, 2010; Shao et al, 2019; Sharma et al, 2016; Vences et al, 2020). However, there exists a paucity of information on the presence of antibiotic‐resistant P. damselae subsp.…”

Section: Discussionmentioning

confidence: 99%

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Pathogenicity potential of antimicrobial‐resistant Photobacterium damselae subsp. damselae isolated from maricultured and wild‐caught fish of the southeast coast of India

Petchimuthu

George

John

et al. 2022

Aquaculture Research

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Antimicrobial resistance and zoonotic pathogen transmission from wild fish to high‐density cage‐cultured fish are two of the emerging concerns in mariculture. Photobacterium damselae is one of the most destructive bacterial pathogens which is responsible for huge economic loss to the cage culture systems. To investigate the above, both wild‐caught and cage‐cultured fish were collected from the southeast coast of India. P. damselae subsp. damselae was isolated and their genotypic profiling was done using a specific multiplex polymerase chain reaction. Out of 280 isolates, 61 isolates were confirmed as P. damselae subsp. damselae. The overall prevalence of P. damselae subsp. damselae in wild fish was significantly higher compared with cage‐cultured fish. Among the wild fish, parrot fish showed more prevalence when compared with other fish. Around 90% of the identified bacterial isolates were multiple antibiotic‐resistant and carried antibiotic resistance genes such as beta‐lactam and streptomycin genes. To know the virulence of P. damselae bacteria isolated from wild‐caught fish to commercially important cage‐cultured fish (cobia and silver pompano) experimentally, a challenging study was carried out. The results revealed that cobia and silver pompano showed 100% and 85% mortality at a bacterial concentration of 3.5 × 104 CFU Fish−1 and 2.75 × 106 CFU Fish−1 respectively. Severe tissue damage and ulceration were also noticed in both fish. In conclusion, the present study found a high rate of AMR in P. damselae subsp. damselae isolated from the fish of the southeast coast of India that exhibit the strong pathogenic potential to cage‐cultured fish.

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Section: Discussionmentioning

confidence: 99%

“…Vences et al, 2020). However, there exists a paucity of information on the presence of antibiotic-resistant P. damselae subsp.…”

mentioning

confidence: 99%

Pathogenicity potential of antimicrobial‐resistant Photobacterium damselae subsp. damselae isolated from maricultured and wild‐caught fish of the southeast coast of India

Petchimuthu

George

John

et al. 2022

Aquaculture Research

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“…The general features of the 7 Pdd genomes sequenced in the present study are described in Table 5. In addition, we retrieved from GenBank database the genomes of 3 Scr + strains (64bp-OG9, 70dps-OG12, and 89dp-OG16) that have been sequenced in our laboratory in a recent study focused on the presence of large multidrug resistance plasmids in this subspecies [26] (Table 1), as well as 13 genomes of Scr − Pdd strains obtained in previous studies in our laboratory ( Table 1). Pdd genomes were compared using RAST tool [49], searching for genes present in Scr + strains and absent from Scr − strains.…”

Section: Identification Of Two Different Genetic Variants Of a Four Gmentioning

confidence: 99%

“…Moreover, it can cause opportunistic infections in humans that may evolve into a fatal outcome [23]. Pdd is considered a genetically diverse subspecies, and horizontal gene transfer largely contributes to the generation of such diversity [22,[24][25][26]. Pdd typically forms green colonies on TCBS medium, but early studies pointed out that 5% of the isolates produced yellow colonies on TCBS [27].…”

Section: Introductionmentioning

confidence: 99%

Diverse Horizontally-Acquired Gene Clusters Confer Sucrose Utilization to Different Lineages of the Marine Pathogen Photobacterium damselae subsp. damselae

Abushattal

Vences

Barca

et al. 2020

Genes

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The ability to metabolize sucrose is a variable trait within the family Vibrionaceae. The marine bacterium Photobacterium damselae subsp. damselae (Pdd), pathogenic for marine animals and humans, is generally described as negative for sucrose utilization (Scr−). Previous studies have reported sucrose-utilizing isolates (Scr+), but the genetic basis of this variable phenotype remains uncharacterized. Here, we carried out the genome sequencing of five Scr+ and two Scr− Pdd isolates and conducted a comparative genomics analysis with sixteen additional Pdd genomes sequenced in previous studies. We identified two different versions of a four-gene cluster (scr cluster) exclusive of Scr+ isolates encoding a PTS system sucrose-specific IIBC component (scrA), a fructokinase (scrK), a sucrose-6-phosphate hydrolase (scrB), and a sucrose operon repressor (scrR). A scrA deletion mutant did not ferment sucrose and was impaired for growth with sucrose as carbon source. Comparative genomics analyses suggested that scr clusters were acquired by horizontal transfer by different lineages of Pdd and were inserted into a recombination hot-spot in the Pdd genome. The incongruence of phylogenies based on housekeeping genes and on scr genes revealed that phylogenetically diverse gene clusters for sucrose utilization have undergone extensive horizontal transfer among species of Vibrio and Photobacterium.

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Photobacterium

Labella

Borrego

2023

Bergey's Manual of Systematics of Archaea and Bacteria

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Pho.to.bac.te'ri.um. Gr. neut. n. phôs , light; Gr. neut. dim. n. bakterion , a small rod; N.L. neut. n. Photobacterium , light (‐producing) bacterium. Proteobacteria / Gammaproteobacteria / Vibrionales / Vibrionaceae / Photobacterium The genus Photobacterium contains 37 species with validated names and 3 species whose names have not been validated. Cells are short bacilli Gram‐stain negative, and nonsporulated. Facultatively anaerobic. Require NaCl for growth (optimal concentration 1–3%). Several species present bioluminescence. Some of them are piezophilic and others are psychrophilic. Isolated from marine environment (seawater and sediment), associated with aquatic animals (mutualism relationships), but some are pathogens for poikilothermic and homeothermic animals. DNA G + C content (mol%) : 38.5–53.6. Type species : Photobacterium phosphoreum Beijerinck 1889 AL (basonym: Micrococcus phosphoreus Cohn 1878).

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Highly Transferable pAQU-Related Plasmids Encoding Multidrug Resistance Are Widespread in the Human and Fish Pathogen Photobacterium damselae subsp. damselae in Aquaculture Areas in the Black Sea

Cited by 4 publications

References 38 publications

Pathogenicity potential of antimicrobial‐resistant Photobacterium damselae subsp. damselae isolated from maricultured and wild‐caught fish of the southeast coast of India

Pathogenicity potential of antimicrobial‐resistant Photobacterium damselae subsp. damselae isolated from maricultured and wild‐caught fish of the southeast coast of India

Diverse Horizontally-Acquired Gene Clusters Confer Sucrose Utilization to Different Lineages of the Marine Pathogen Photobacterium damselae subsp. damselae

Photobacterium

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