Turbulent opposed jet (TOJ) burners are an interesting test case for fundamental combustion research and a good benchmark for the available modelling approaches. However, these opposed jet flames strongly depend on the turbulence generation inside the nozzle, which is usually achieved through a perforated plate upstream of the nozzle exit. The present work investigates the flow from these perforated plates and the subsequent turbulence generation in great detail. We present results from highly-resolved large eddy simulations (LES) of the in-nozzle flow in turbulent opposed jets alongside state-of-the-art particle image velocimetry (PIV) at standard and high repetition rates taken inside a glass nozzle. The in-nozzle PIV data provides the LES inflow conditions with unprecedented detail, which are used to follow the initial jet development behaviour known from PIV, before jet coalescence, turbulence production and decay further downstream in the nozzles are successfully predicted. In regions where the PIV experiment suffers from inherent limitations like reflections and the velocity bias, the LES data is available to still obtain a detailed picture of the flow. The sensitivity of the simulations to various physical and numerical parameters is discussed in detail. Results from LES and PIV are compared Flow Turbulence Combust (2011) 87:425-447 qualitatively and quantitatively in terms of first and second moments of velocity, temporal autocorrelations, and energy density spectra. Significant deviations are found in the frequency (20%) and strength of vortex shedding from the inlet plane only, whereas the qualitative and quantitative agreement between simulation and experiment is otherwise excellent throughout, implying that a successful large eddy simulation of a turbulent opposed jet can be performed in a domain that includes the perforated plates.