Deflection and vibration play an important role in the serviceability of bridges. Their limits are specified in design codes either for consideration or as requirements. To promote understanding of the role of deflection and vibration limits, a comprehensive literature review is presented and comparison is made to propose the rational method to control bridge vibration for human comfort. To investigate the vibration and deflection criteria and applicability for bridges, actual bridges to represent typical bridge types are selected for experimental tests and theoretical study. Comparisons of the experimental results of deflection, acceleration, and frequency with the theoretical results are performed to verify the validity of the theoretical models. To ensure the applicability of the design code, bridges are redesigned so that they have minimum capacities as required by the design code. Finally, the vibration parameters of actual bridges and bridges using minimum sections are compared with current design limits and proposed criteria.