Hikui disease in nine koi carp (<i>Cyprinus carpio</i>): first description of a cutaneous perivascular wall tumour

Sirri, Rubina; Pretto, Tobia; Montesi, Francesco; Berton, Valeria; Mandrioli, Luciana; Barbè, Tim

doi:10.1111/vde.12338

Cited by 6 publications

(10 citation statements)

References 13 publications

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“…Immunohistochemistry represented a useful diagnostic tool also in perivascular wall tumors, which were recently found and described in fish (Sirri et al. ).…”

Section: Discussionmentioning

confidence: 98%

Immunohistochemical Study of Four Fish Tumors

et al. 2019

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The present study supports the usefulness of ancillary techniques, such as immunohistochemistry, as a valid diagnostic tool in the field of fish oncology. The immunohistochemical patterns observed in four neoplasms on four individual teleosts belonging to different species are described. Cytokeratin, vimentin, actin, S100, calretinin, and Melan-A antibodies were used. Diagnoses of papilloma in a Bream Abramis brama, fibroma in a Sand Steenbras Lithognathus mormyrus, schwannoma in a Crucian Carp Carassius carassius, and melanoma in a spontaneously inbred Xiphophorus hybrid were made. Diagnosis of tumors in fish is not always easy to carry out, and the tool provided by antibodies used on mammalian tissue is essential for obtaining definitive, unambiguous, and inexpensive identification.

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“…Immunohistochemistry represented a useful diagnostic tool also in perivascular wall tumors, which were recently found and described in fish (Sirri et al. ).…”

Section: Discussionmentioning

confidence: 98%

Immunohistochemical Study of Four Fish Tumors

et al. 2019

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“…Taking the embryological origin into account, the histological features of the presented cases were compatible in most cases with a diagnosis of SCSTs and were observed mainly in female fish, in line with what reported for koi populations in Switzerland (Ott Knüsel et al., 2016). The antibodies employed in this study were selected on the basis of their known cross‐reactivity in fish tissues (Sirri et al., 2008; Sirri et al., 2010; Sirri et al, 2016; Ott Knüsel et al., 2016; Budai et al., 2020) and, with the purpose to deepen the spectrum of gonadic neoplasm, some other antibodies routinely used in mammalian ovary and testis tumours diagnosis available in our laboratories were added to the panel that works in fish. In normal mammalian gonadic tissues, the coelomic epithelium is positive for both cytokeratins and vimentin, while the granulosa cells of primordial follicles express only cytokeratins in the foetal ovary, while co‐expressing cytokeratins and vimentin in the adult ovary.…”

Section: Discussionmentioning

confidence: 99%

“…Immunohistochemistry was performed with a panel of antibodies (vimentin, CD117, placental alkaline phosphatase—PLAP, AE1/AE3 cytokeratin, E‐cadherin, proliferating cell nuclear antigen—PCNA, müllerian‐inhibiting substance—MIS, GATA4 and Inhibin‐α), used in diagnostic oncology of mammalian gonadic tumours. Some of them have been already tested in normal fish tissues and especially in cyprinids in previous studies (Ott Knüsel et al., 2016; Sirri et al., 2008, 2010, 2016).…”

Section: Methodsmentioning

confidence: 99%

“…They have a long lifespan, high economic value and high emotional value as pets due to their tame characters (Ott Knüsel et al., 2016). To date, only a few studies have reported the occurrence of tumours in koi carp, describing neoplasms of the skin, gills, liver, gonads and kidney (Ishikawa & Takayama, 1977; Knüsel et al., 2007; Ott Knüsel et al., 2015, 2016; Sirri et al., 2010, 2016, 2017). Among the abdominal neoplasms, hepatic carcinoma, renal adenocarcinoma, intestinal carcinoma, retroperitoneal hemangiosarcoma and gonadal tumours are those reported in literature for koi and common carp (Down & Leatherland, 1989; Fregeneda‐Grandes et al., 2010; Harshbarger, 2001; Hyatt et al., 2013; Ito et al., 1984; Leatherland & Down, 2001; Lewbart et al., 1988; Lewisch et al., 2014; Ott Knüsel et al., 2016; Raidal et al., 2006; Romano et al., 2014; Yasumoto et al., 2015).…”

Section: Introductionmentioning

confidence: 99%

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Histological and immunohistochemical characterization of 17 gonadal tumours in koi carp (Cyprinus carpio koi)

Sirri

Tura

Budai

et al. 2020

Journal of Fish Diseases

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Reports on abdominal tumours in koi carp are scarce and most are from the gonads. Their histological diagnosis is challenging due to the occurrence of mixed populations of neoplastic cells and the few availability of cross‐reactive antibodies in fish tissues. The present study aims to provide a histopathological characterization of seventeen gonadal tumours, enriched by a wide antibody panel (vimentin, CD117, placental alkaline phosphatase—PLAP, AE1/AE3 cytokeratin, E‐cadherin, proliferating cell nuclear antigen—PCNA, müllerian‐inhibiting substance—MIS, GATA4 and Inhibin‐α) applied on whole and tissue microarray (TMA) sections. Abdominal enlargement was associated with tumours filling 30%–80% of the abdominal cavity; frequently, the gonads had been completely replaced by neoplastic tissue. Twelve cases were characterized as sex cord‐stromal tumours (SCSTs), three as germ cell tumours (GCTs), one as mixed germ cell sex cord‐stromal tumour (MGCSCST) and one as carcinoma. By immunohistochemistry, PLAP enabled confirmation of GCTs, ovarian carcinoma and the objective identification of a further cell component in 8 out of the 12 SCSTs that were reclassified as mixed tumours. The use of an immunohistochemical panel can help in refining the histological diagnosis, but the morphological diagnosis still represents the main tool for the characterization of these tumours in koi carp.

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“…A majority of the chromatophoromas contained cells with few or no cytoplasmic pigment granules, making these regions difficult to distinguish from other dermal spindle cell tumors. Chromatophoromas sometimes produce no pigment, and histologic distinction from other spindle cell tumors, such as the perivascular wall tumors formed in Hikui disease, 11 can be challenging. IHC could be used in these poorly pigmented and non-pigmented tumors; however, IHC analysis of neoplasms in fish, and specifically neoplasms of chromatophore origin, are not well-documented.…”

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confidence: 99%

Immunohistochemical analysis of pigment cell tumors in two cyprinid species

et al. 2019

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Pigment cell tumors, also known as chromatophoromas, are cutaneous spindle cell neoplasms originating from pigment cells (chromatophores) in the dermis of teleosts, amphibians, and reptiles. Chromatophoromas share similar histologic morphology to other spindle cell tumors and are not always pigmented. Therefore, immunohistochemical analysis may be useful in distinguishing these neoplasms from tumors of other cellular origin when poorly pigmented. We performed 3 immunohistochemistry assays (PNL-2, melan A, and SOX10) on 8 cutaneous neoplasms from 8 teleosts diagnosed as chromatophoromas based on histologic morphology. Semiquantitative analysis of immunoreactivity was evaluated on each immunohistochemical assay using a 0–3 scale. PNL-2 exhibited mild-to-moderate (1 or 2) immunoreactivity in 7 of the cases, and resident chromatophores (internal control) were also immunoreactive in these cases. Melan A exhibited mild-to-moderate (1 or 2) immunoreactivity in 4 cases (and with resident chromatophores in these cases); SOX10 was not immunoreactive in any cases. Our results indicate that PNL-2 may be a useful marker in teleosts to distinguish tumors of chromatophore origin. Melan A could also be useful, but appears to be less sensitive, and SOX10 is likely not a useful marker for these neoplasms in teleosts.

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Hikui disease in nine koi carp (Cyprinus carpio): first description of a cutaneous perivascular wall tumour

Cited by 6 publications

References 13 publications

Immunohistochemical Study of Four Fish Tumors

Immunohistochemical Study of Four Fish Tumors

Histological and immunohistochemical characterization of 17 gonadal tumours in koi carp (Cyprinus carpio koi)

Immunohistochemical analysis of pigment cell tumors in two cyprinid species

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