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Symbiotic microorganisms living in the digestive tracts of invertebrates can be crucial in host-symbiont interactions, as they play fundamental roles in important biological processes. Urbanization-related habitat alteration and disturbance, however, considerably affect the environment of host insects, from which their gut microbiota is derived. Still, relatively few studies, all on flying insects, have assessed the impact of urbanization on the gut microbiota of insects. Here, we compared the gut bacterial microbiota in rural and urban individuals of a flightless ground beetle, Carabus convexus , using next generation sequencing. Across the 48 gut samples we identified 1163 different bacterial operational taxonomic units (OTUs), forming significantly different gut bacterial communities in rural versus urban beetles. The taxonomic diversity of the gut bacterial microbiota expressed by the Hill numbers was significantly higher in rural than urban individuals, as well as in rural males vs. females. Smaller differences were found in functional diversity, assessed by the Rao’s quadratic entropy which was marginally significantly higher in urban than rural beetles. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-75864-6.
Symbiotic microorganisms living in the digestive tracts of invertebrates can be crucial in host-symbiont interactions, as they play fundamental roles in important biological processes. Urbanization-related habitat alteration and disturbance, however, considerably affect the environment of host insects, from which their gut microbiota is derived. Still, relatively few studies, all on flying insects, have assessed the impact of urbanization on the gut microbiota of insects. Here, we compared the gut bacterial microbiota in rural and urban individuals of a flightless ground beetle, Carabus convexus , using next generation sequencing. Across the 48 gut samples we identified 1163 different bacterial operational taxonomic units (OTUs), forming significantly different gut bacterial communities in rural versus urban beetles. The taxonomic diversity of the gut bacterial microbiota expressed by the Hill numbers was significantly higher in rural than urban individuals, as well as in rural males vs. females. Smaller differences were found in functional diversity, assessed by the Rao’s quadratic entropy which was marginally significantly higher in urban than rural beetles. Supplementary Information The online version contains supplementary material available at 10.1038/s41598-024-75864-6.
Measuring complexity in multidimensional systems with high degrees of freedom and a variety of types of information, remains an important challenge. The complexity of a system is related to the number and variety of components, the number and type of interactions among them, the degree of redundancy, and the degrees of freedom of the system. Examples show that different disciplines of science converge in complexity measures for low and high dimensional problems. For low dimensional systems, such as coded strings of symbols (text, computer code, DNA, RNA, proteins, music), Shannon’s Information Entropy (expected amount of information in an event drawn from a given distribution) and Kolmogorov‘s Algorithmic Complexity (the length of the shortest algorithm that produces the object as output), are used for quantitative measurements of complexity. For systems with more dimensions (ecosystems, brains, social groupings), network science provides better tools for that purpose. For highly complex multidimensional systems, none of the former methods are useful. Here, information related to complexity can be used in systems, ranging from the subatomic to the ecological, social, mental and to AI. Useful Information Φ (Information that produces thermodynamic free energy) can be quantified by measuring the thermodynamic Free Energy and/or useful Work it produces. Complexity can be measured as Total Information I of the system, that includes Φ, useless information or Noise N, and Redundant Information R. Measuring one or more of these variables allows quantifying and classifying complexity. Complexity and Information are two windows overlooking the same fundamental phenomenon, broadening out tools to explore the deep structural dynamics of nature at all levels of complexity, including natural and artificial intelligence.
Measuring complexity in multidimensional systems with high degrees of freedom and a variety of types of information, remains an important challenge. The complexity of a system is related to the number and variety of components, the number and type of interactions among them, the degree of redundancy, and the degrees of freedom of the system. Examples show that different disciplines of science converge in complexity measures for low and high dimensional problems. For low dimensional systems, such as coded strings of symbols (text, computer code, DNA, RNA, proteins, music), Shannon’s Information Entropy (expected amount of information in an event drawn from a given distribution) and Kolmogorov‘s Algorithmic Complexity (the length of the shortest algorithm that produces the object as output), are used for quantitative measurements of complexity. For systems with more dimensions (ecosystems, brains, social groupings), network science provides better tools for that purpose. For highly complex multidimensional systems, none of the former methods are useful. Here, information related to complexity can be used in systems, ranging from the subatomic to the ecological, social, mental and to AI. Useful Information Φ (Information that produces thermodynamic free energy) can be quantified by measuring the thermodynamic Free Energy and/or useful Work it produces. Complexity can be measured as Total Information I of the system, that includes Φ, useless information or Noise N, and Redundant Information R. Measuring one or more of these variables allows quantifying and classifying complexity. Complexity and Information are two windows overlooking the same fundamental phenomenon, broadening out tools to explore the deep structural dynamics of nature at all levels of complexity, including natural and artificial intelligence.
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