Hinokitiol Selectively Enhances the Antibacterial Activity of Tetracyclines against Staphylococcus aureus

Le, Chun-Yan; Ye, Yu-Jian; Xu, Jian; Li, Lei; Feng, Xi-Qing; Chen, Ni-Pi; Zhu, Bingqi; Ding, Zhongxiang

doi:10.1128/spectrum.03205-22

Cited by 4 publications

(3 citation statements)

References 49 publications

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“…Several studies have shown that Hi reduces tumor growth by activating apoptosis and autophagy [25,56,58]. Hi has been shown to possess significant antibacterial activity against a range of pathogenic bacteria [23,59]. In addition to its antibacterial activities, Hi has also been assessed against a variety of fungal species, including Aspergillus and Candida species [60,61].…”

Section: Discussionmentioning

confidence: 99%

“…Among the various extracts derived from cypress wood, hinokitiol (β-thujaplicin), a natural extract from the Japan cypress, is a monoterpene compound. Hinokitiol has been shown to exert a wide range of biological and pharmacological effects, including antimicrobial and ant-inflammatory properties [21][22][23], neuroprotective activities [24], and anticancer effects [25].…”

Section: Introductionmentioning

confidence: 99%

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Natural Compounds with Antifungal Properties against Candida albicans and Identification of Hinokitiol as a Promising Antifungal Drug

Camaioni,

Ustyanowski,

Buisine

et al. 2023

Antibiotics

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Candida albicans is an opportunistic yeast that causes most fungal infections. C. albicans has become increasingly resistant to antifungal drugs over the past decade. Our study focused on the identification of pure natural compounds for the development of antifungal medicines. A total of 15 natural compounds from different chemical families (cinnamic derivatives, aromatic phenols, mono- and sesquiterpenols, and unclassified compounds) were screened in this study. Among these groups, hinokitiol (Hi), a natural monoterpenoid extracted from the wood of the cypress family, showed excellent anti-C. albicans activity, with a MIC value of 8.21 µg/mL. Hi was selected from this panel for further investigation to assess its antifungal and anti-inflammatory properties. Hi exhibited significant antifungal activity against clinically isolated fluconazole- or caspofungin-resistant C. albicans strains. It also reduced biofilm formation and hyphal growth. Treatment with Hi protected Caenorhabditis elegans against infection with C. albicans and enhanced the expression of antimicrobial genes in worms infected with C. albicans. Aside from its antifungal activities against C. albicans, Hi challenge attenuated the LPS-induced expression of pro-inflammatory cytokines (IL-6, IL-1β, and CCL-2) in macrophages. Overall, Hi is a natural compound with antifungal and anti-inflammatory properties, making Hi a promising platform with which to fight against fungal infections.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Natural Compounds with Antifungal Properties against Candida albicans and Identification of Hinokitiol as a Promising Antifungal Drug

Camaioni,

Ustyanowski,

Buisine

et al. 2023

Antibiotics

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show abstract

“…Antimicrobial activity assays were performed by the minimum inhibitory concentration (MIC) method and time-kill experiments, as described previously [44]. The MIC was defined as the lowest concentration of drug with invisible bacterial growth after incubation at 37 • C for 18-20 h. DMSO (3.2%) was used as a negative control, and vancomycin was used as a positive control of bactericidal antibiotic.…”

Section: Antimicrobial Activity Assaysmentioning

confidence: 99%

Myricetin Acts as an Inhibitor of Type II NADH Dehydrogenase from Staphylococcus aureus

Zhou,

Chen,

et al. 2024

Molecules

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Background: Staphylococcus aureus is a common pathogenic microorganism in humans and animals. Type II NADH oxidoreductase (NDH-2) is the only NADH:quinone oxidoreductase present in this organism and represents a promising target for the development of anti-staphylococcal drugs. Recently, myricetin, a natural flavonoid from vegetables and fruits, was found to be a potential inhibitor of NDH-2 of S. aureus. The objective of this study was to evaluate the inhibitory properties of myricetin against NDH-2 and its impact on the growth and expression of virulence factors in S. aureus. Results: A screening method was established to identify effective inhibitors of NDH-2, based on heterologously expressed S. aureus NDH-2. Myricetin was found to be an effective inhibitor of NDH-2 with a half maximal inhibitory concentration (IC50) of 2 μM. In silico predictions and enzyme inhibition kinetics further characterized myricetin as a competitive inhibitor of NDH-2 with respect to the substrate menadione (MK). The minimum inhibitory concentrations (MICs) of myricetin against S. aureus strains ranged from 64 to 128 μg/mL. Time–kill assays showed that myricetin was a bactericidal agent against S. aureus. In line with being a competitive inhibitor of the NDH-2 substrate MK, the anti-staphylococcal activity of myricetin was antagonized by MK-4. In addition, myricetin was found to inhibit the gene expression of enterotoxin SeA and reduce the hemolytic activity induced by S. aureus culture on rabbit erythrocytes in a dose-dependent manner. Conclusions: Myricetin was newly discovered to be a competitive inhibitor of S. aureus NDH-2 in relation to the substrate MK. This discovery offers a fresh perspective on the anti-staphylococcal activity of myricetin.

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Mechanistic insights and therapeutic applications of Hinokitiol in Inflammation, Antimicrobial therapy, and Cancer

Karunakar,

Cheriyan,

Velmurugan

et al. 2024

Pharmacological Research - Modern Chinese Medicine

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Hinokitiol Selectively Enhances the Antibacterial Activity of Tetracyclines against Staphylococcus aureus

Cited by 4 publications

References 49 publications

Natural Compounds with Antifungal Properties against Candida albicans and Identification of Hinokitiol as a Promising Antifungal Drug

Natural Compounds with Antifungal Properties against Candida albicans and Identification of Hinokitiol as a Promising Antifungal Drug

Myricetin Acts as an Inhibitor of Type II NADH Dehydrogenase from Staphylococcus aureus

Mechanistic insights and therapeutic applications of Hinokitiol in Inflammation, Antimicrobial therapy, and Cancer

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